Modulatory effects of laurel-leaf cistus (Cistus laurifolius) ethanolic extract on innate immune responses and disease resistance in common carp (Cyprinus carpio)

Bi̇len, Soner; Ali, Gamaia Ali Mohamed; Amhamed, Iman Daw; Almabrok, Ahmed Alhadi

doi:10.1016/j.fsi.2021.07.001

Cited by 19 publications

(8 citation statements)

References 57 publications

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“…Herbal additives potentiate to alter cytokine expression in fish intestine (Hoseinifar et al, 2020b;Hoseinifar et al, 2020d). It has been demonstrated that upregulation in the intestinal pro-inflammatory cytokines following herbal additive administration has been accompanied by better disease resistance after a pathogenic challenge (Noor-Ul et al, 2020;Bilen et al, 2021;Ibrahim et al, 2021). The present results suggest that 100 mg/kg HE induces several immune genes in the fish intestine, which may be interpreted as improved intestinal health.…”

Section: Discussionmentioning

confidence: 99%

“…are known for their health benefits in fish and herbal feed additives have been found to increase their proportion in fish gut (Adel et al, 2015;Adel et al, 2016). Besides, herbal additives have critical roles in intestinal immunity by stimulating transcriptions of various genes including cytokines and antimicrobial peptide (Gora et al, 2018;Sun et al, 2018;Hoseinifar et al, 2020c;Bilen et al, 2021); thereby these additives increase disease resistance (Adeshina et al, 2021;Lumsangkul et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary methanolic extract of hyssop, Hyssopus officinalis, on growth performance, hepatic antioxidant, humoral and intestinal immunity, and intestinal bacteria of rainbow trout, Oncorhynchus mykiss

et al. 2022

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The aims of the present study were to assess the effects of Hyssop, Hyssopus officinalis, methanolic extract (HE) on growth performance, hepatic oxidative status, humoral and intestinal immunity, and intestinal bacteria of rainbow trout, Oncorhynchus mykiss. Fish were allocated into twelve tanks for four treatments, receiving diets containing 0, 100, 250, and 500 mg/kg HE for eight weeks. The results showed that dietary HE supplementation induced no significant differences in the growth performance, feed efficiency, and hematological parameters (P > 0.05). HE supplementation significantly increased total leukocyte count and the highest count was observed in 250 mg/kg HE treatment (P < 0.001). Fish in 250 and 500 mg/kg HE treatments exhibited significantly lower lymphocyte (P = 0.001) and higher neutrophil (P = 0.002) percentages; the former exhibited a significantly higher monocyte percentage (P = 0.021). Hepatic superoxide dismutase (100 and 250 mg/kg HE; P < 0.001), glutathione peroxidase (100 and 250 mg/kg HE; P = 0.001), glutathione reducatse (all HE treatments; P < 0.001), and reduced glutathione (250 mg/kg HE; P = 0.046) significantly increased, whereas hepatic malondialdehyde levels (250 and 500 mg/kg HE; P = 0.007) significantly decreased in HE-treated fish. Plasma total protein, albumin, globulin, lysozyme, and alternative complement significantly increased in 250 and 500 mg/kg HE treatments and plasma total Ig significantly increased in 250 mg/kg HE treatment. Quantitative real time PCR found no Streptococcus iniae, Lactococcus garvieae, Aeromonas hydrophila, Yersinia ruckeri, and Vibrio anguillarum in the fish intestines in any treatments. Lactobacillus sp. was detected in the fish intestinal samples, but there were no significant differences among the treatments (P = 0.352). Intestinal defensin (P = 0.044) and interleukin-1 beta (P = 0.0.035) expressions were significantly up-regulated in 100 mg/kg HE; intestinal interleukin-10 (P < 0.001) and tumor necrosis factor-alpha (P < 0.001) expressions were significantly up-regulated in 100 and 500 mg/kg HE; whereas, intestinal interleukin-6 expression was significantly (P = 0.009) up-regulated in 250 mg/kg HE treatments. It is concluded that HE is able to stimulate humoral and intestinal immune responses and hepatic antioxidant capacity. HE effective concentration in rainbow trout may be in the range of 100-250 mg/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary methanolic extract of hyssop, Hyssopus officinalis, on growth performance, hepatic antioxidant, humoral and intestinal immunity, and intestinal bacteria of rainbow trout, Oncorhynchus mykiss

et al. 2022

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“…Some plants such as oak ( Quercus aegilops ), 66 ginger, 67 lemon ( Citrus limon ), 68 ferula ( F. asafoetida ), 26 and lamb's quarters ( Chenopodium album ) 69 improved carp's growth more than ≥100% relative to control depending on the % inclusion (Figures 6–8). On the other hand, plants such as pistachio ( Pistacia vera ), 70 common mallow, 32 laurel‐leaf cistus, 53 and pepper rosemary ( L. sidoides ) 31 can depress the growth of carp beyond −20% depending on the inclusion rate. It could be due to collateral introduction of anti‐nutritional factors (ANFs), which is widespread in plant‐origin feedstuff.…”

Section: Resultsmentioning

confidence: 99%

“…Digestive enzyme activities are directly associated with growth performance in fish as they are responsible for the hydrolysis of protein, lipids and carbohydrates. Some PFAs contain coumarins, flavonoids, terpenoids and sugars which can trigger amylase activity in fish 53,75 . It was, however, revealed that not all PFAs can enhance the lipase and amylase activities of carp.…”

Section: Resultsmentioning

confidence: 99%

Plant‐based feed additives in Cyprinus carpio aquaculture

Kuebutornye

Roy

Folorunso

et al. 2023

Reviews in Aquaculture

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Species feeding lower at the food web like cyprinids (carp) are often advocated for sustainable aquaculture. However, their gastrointestinal biology (lack of stomach) has limited their acid‐peptic digestion, acid lysis of plants and invertebrate tissues, and phosphorus uptake. This review presents data from 118 articles on the use of plant‐based feed additives (PFAs) in Cyprinus carpio (common carp). Our review shows that most PFA research in carp is focused on improving feed and nutrient utilization efficiency along various levels (in vivo digestibility, metabolizability to in situ nutrient loading), while other half is focused on ensuring animal welfare standards (biomass, immunity, welfare). Several reviewed plant‐based additives however tend to do both. The PFAs include essential oils (EOs), plant‐part extracts (PPEs), medicinal plants (herbs) and spices. The effective inclusion rate of EOs is lower (0.5%–0.75%). Whereas PPEs, herbs and spices work at a generic inclusion rate of 2%–5% in carp feeds. Even at an optimum inclusion rate, there are factors controlling PFAs efficacy such as methods of preparation (reviewed). PFAs like lemon beebrush, geranium, dill, Rosemary pepper and common mallow with/without certain pre‐conditions are better to avoid in cyprinid diets. Although certain PFAs may potentially alter organoleptic qualities of carp flesh or might assist in training carp immunity, information on these aspects is limited. The review also provides a quick guide to researchers and feed companies interested in exploring the potential of PFAs in C. carpio aquaculture. Potential PFAs that could be valuable for cyprinids, but not yet explored are also listed.

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“…Plant‐based feed additives have a few side effects and are readily available, making them cheap and sustainable natural antioxidants and immunostimulants in aquaculture (Elumalai et al, 2021). Dietary supplementation with plant‐based feed additives such as oak acorn (Abdel‐Tawwab et al, 2021), olive leaves (Zemheri‐Navruz et al, 2019), cistus (Bilen et al, 2021), and common mallow (Bilen et al, 2020) have been shown to enhance health status of common carp. In recent years, studies are also focused on dietary supplementation with plant additives to improve fish stress responses caused by high stocking density (Bacchetta et al, 2020; Mahmoud et al, 2021; Shourbela et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Dietary Artemisia, Artemisia annua , supplementation improves common carp welfare under high stocking density

Hoseini

Aydın

Hoseinifar

et al. 2022

Aquaculture Research

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Intensive aquaculture supports higher production per unit of area, but it may be associated by stress and health problems in fish. Thus, researches are continuing to find applicable methods for suppressing such negative effects. Dietary supplementation with medicinal plant is known as a promising method in this field. Accordingly, the present study aimed at assessing the benefits of dietary Artemisia, Artemisia annua, supplementation (0, 2.5, 5 and 10 g/kg) on growth performance, stress, antioxidant and immunological responses of common carp, Cyprinus carpio, held at a high stocking density (10 g/L vs. 3 g/L) for 70 days. Increase in fish stocking density significantly (p < 0.05) reduced growth performance, haematological parameters (red blood cell, haematocrit, haemoglobin, leukocyte and lymphocyte) and immune responses (total protein, complement and total immunoglobulin), but increased neutrophil, lysozyme, stress responses (cortisol, glucose, aspartate aminotransferase and lactate dehydrogenase), antioxidant enzymes’ activities (superoxide dismutase, catalase and glutathione peroxidase) and lipid peroxidation. Artemisia supplementation significantly (p < 0.05) restored haematological parameters, leukocyte count and mitigated the changes in lymphocyte, neutrophil, stress and immunological parameters, antioxidant enzymes’ activities and lipid peroxidation. Based on the results, dietary Artemisia supplementation is not capable to restore growth retardation of common carp under high stocking density; however, it mitigates the adverse effects of high stocking density on haematological, antioxidant and immunological parameters. Supplementation at 5 g/kg is recommended for carp feed formulation.

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Modulatory effects of laurel-leaf cistus (Cistus laurifolius) ethanolic extract on innate immune responses and disease resistance in common carp (Cyprinus carpio)

Cited by 19 publications

References 57 publications

Effects of dietary methanolic extract of hyssop, Hyssopus officinalis, on growth performance, hepatic antioxidant, humoral and intestinal immunity, and intestinal bacteria of rainbow trout, Oncorhynchus mykiss

Effects of dietary methanolic extract of hyssop, Hyssopus officinalis, on growth performance, hepatic antioxidant, humoral and intestinal immunity, and intestinal bacteria of rainbow trout, Oncorhynchus mykiss

Plant‐based feed additives in Cyprinus carpio aquaculture

Dietary Artemisia, Artemisia annua , supplementation improves common carp welfare under high stocking density

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