Modulatory Effects of Alpha-Mangostin Mediated by SIRT1/3-FOXO3a Pathway in Oxidative Stress-Induced Neuronal Cells

Ruankham, Waralee; Suwanjang, Wilasinee; Phopin, Kamonrat; Songtawee, Napat; Prachayasittikul, Virapong; Prachayasittikul, Supaluk

doi:10.3389/fnut.2021.714463

Cited by 11 publications

(4 citation statements)

References 47 publications

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“…Although α-MG is reported to exert antioxidant and anti-inflammatory effects [ 36 , 37 ], our results demonstrate that it can also promote ROS accumulation ( Figure 6 A–C) as previously seen in other cell types [ 8 , 38 ]. Oxidative stress primes RBCs for eryptosis, especially given the vulnerability of these cells to oxygen damage.…”

Section: Discussionsupporting

confidence: 84%

Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood Cells

Alghareeb,

Alsughayyir,

Alfhili

2023

Molecules

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Background: Chemotherapy-related anemia is prevalent in up to 75% of patients, which may arise due to hemolysis and eryptosis. Alpha-mangostin (α-MG) is a polyphenolic xanthonoid found in the mangosteen tree (Garcinia mangostana) whose antitumor medicinal properties are well-established. Nevertheless, the potential toxic effects of α-MG on red blood cells (RBCs) have, as of yet, not been as well studied. Methods: RBCs were exposed to 1–40 μM of α-MG for 24 h at 37 °C. Hemolysis and related markers were measured using colorimetric assays, eryptotic cells were identified through Annexin-V-FITC, Ca2+ was detected with Fluo4/AM, and oxidative stress was assessed through H2DCFDA using flow cytometry. The toxicity of α-MG was also examined in the presence of specific signal transduction inhibitors and in whole blood. Results: α-MG at 10–40 μM caused dose-dependent hemolysis with concurrent significant elevation in K+, Mg2+, and LDH leakage, but at 2.5 μM it significantly increased the osmotic resistance of cells. A significant increase was also noted in Annexin-V-binding cells, along with intracellular Ca2+, oxidative stress, and cell shrinkage. Moreover, acetylcholinesterase activity was significantly inhibited by α-MG, whose hemolytic potential was significantly ameliorated by the presence of BAPTA-AM, vitamin C, NSC23766, and isosmotic sucrose but not urea. In whole blood, α-MG significantly depleted intracellular hemoglobin stores and was selectively toxic to platelets and monocytes. Conclusions: α-MG possesses hemolytic and eryptotic activities mediated through Ca2+ signaling, Rac1 GTPase activity, and oxidative injury. Also, α-MG leads to accelerated cellular aging and specifically targets platelet and monocyte populations in a whole blood milieu.

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Section: Discussionsupporting

confidence: 84%

Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood Cells

Alghareeb,

Alsughayyir,

Alfhili

2023

Molecules

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“…In general, the decrease of mitochondrial membrane potential is closely related to the imbalance of intracellular redox reactions [ 34 , 35 ]. However, the imbalance of redox is bound to lead to the accumulation of ROS [ 36 ], resulting in the damage of intracellular biological macromolecules, such as nucleic acid fragmentation and protein degeneration [ 37 ]. Therefore, TUNEL-DAPI staining was used to detect DNA damage in cells after virus infection [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of H1N1 Influenza Virus-induced Apoptosis by Ebselen Through ROS-mediated ATM/ATR Signaling Pathways

Chen

Ren

et al. 2022

Biol Trace Elem Res

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Influenza A viruses can cause global outbreaks and seasonal pandemics. However, the use of conventional anti-influenza drugs leads to an increase in drug-resistant mutations in influenza viruses worldwide. Therefore, numerous studies have focused on developing effective anti-influenza drugs. It is feasible to treat influenza by targeting influenza-mediated oxidative damage. Ebselen is a synthetic organoselenium compound which provides glutathione peroxidase-like activity. It has been shown to play a role in anti-influenza therapy, but the mechanism remains to be further explored. This experiment verified the anti-influenza effect of ebselen. CCK-8 and PCR showed that ebselen had a significant inhibitory effect on virus replication compared with the virus group. In addition, the mechanistic investigations revealed that ebselen could inhibit influenza-mediated apoptosis, mitochondrial damage, accumulation of reactive oxygen species, and DNA breakage. At the same time, ebselen significantly inhibited the phosphorylation of ATM and ATR and promoted the activation of PARP and Caspase-3. Ebselen, on the other hand, reduced the inflammatory response caused by influenza. These results suggest that ebselen is a promising inhibitor for H1N1.

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“…The oxidant scavenging effects of α-mangostin may be a result from its xanthone structure [ 31–33 ]. The structure has been proven to possess several antioxidant mechanisms, including reactive oxygen species quenching, cellular antioxidant defence systems stimulation or prevention of lipid peroxidation [ 32–34 ].…”

Section: Resultsmentioning

confidence: 99%

The utilization of mangosteen pericarp extract for anticoccidial drug replacement in broiler feed

Sriboonyong

Poommarin

Sittiya

et al. 2022

International Journal of Veterinary Science and Medicine

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The use of anticoccidial drugs in broilers has led to concerns, especially the drug residues in meat and the occurrence of drug resistance. This study aimed to extract, standardize, quantify and utilize mangosteen pericarp extract (MPE) containing α-mangostin as a replacement for anticoccidial drugs in broiler feed. The pericarp was acquired from different areas of Thailand and used for extraction and standardization. The antioxidant activity of the extract was evaluated. The extract was formulated into granules, and the flowability and stability of the granules were assessed. The MPE formulation was added to the broiler feed and then fed to the broilers that were infected with Eimeria tenella . The growth rate and intestinal lesion score (post-mortem) of the broilers were assessed. The pericarp obtained passed the identification test and phytochemical analyses. The active compound, α-mangostin, was best extracted using 95% ethanol. The MPE had superior antioxidant activity compared to standard antioxidants. Granules of the extract formulated with Avicel® PH102 provided desirable flowability and stability. The broilers fed with the feed containing 500 mg/kg α-mangostin showed a similar growth rate and post-mortem lesion score compared with the control group and those that received feed containing 60 mg/kg salinomycin. Our findings demonstrated that MPE with a high content of the active compound could be developed and used in place of anticoccidial drugs in the broiler feed.

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Modulatory Effects of Alpha-Mangostin Mediated by SIRT1/3-FOXO3a Pathway in Oxidative Stress-Induced Neuronal Cells

Cited by 11 publications

References 47 publications

Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood Cells

Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood Cells

Inhibition of H1N1 Influenza Virus-induced Apoptosis by Ebselen Through ROS-mediated ATM/ATR Signaling Pathways

The utilization of mangosteen pericarp extract for anticoccidial drug replacement in broiler feed

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