Modulatory effect of low-shear modeled microgravity on stress resistance, membrane lipid composition, virulence, and relevant gene expression in the food-borne pathogen Listeria monocytogenes

Sheet, Sunirmal; Sathishkumar, Yesupatham; Ghosh, Kuntal; Choi, Misook; Shim, Kwan Seob; Lee, Yang Soo

doi:10.1016/j.enzmictec.2019.109440

Cited by 16 publications

(12 citation statements)

References 58 publications

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“…The increased virulence and antimicrobial resistance are probably due to elevated mutation rates in space. Listeria monocytogenes showed reduced virulence and lower tolerance towards heat and acid conditions after HARV cultivation, while the cold tolerance was increased [ 75 ]. In addition, decreased expression levels of heat stress and virulence-related genes and an upregulation of cold stress genes were found [ 75 ].…”

Section: Resultsmentioning

confidence: 99%

Current Knowledge about the Impact of Microgravity on Gene Regulation

Corydon

Schulz

Richter

et al. 2023

Cells

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Microgravity (µg) has a massive impact on the health of space explorers. Microgravity changes the proliferation, differentiation, and growth of cells. As crewed spaceflights into deep space are being planned along with the commercialization of space travelling, researchers have focused on gene regulation in cells and organisms exposed to real (r-) and simulated (s-) µg. In particular, cancer and metastasis research benefits from the findings obtained under µg conditions. Gene regulation is a key factor in a cell or an organism’s ability to sustain life and respond to environmental changes. It is a universal process to control the amount, location, and timing in which genes are expressed. In this review, we provide an overview of µg-induced changes in the numerous mechanisms involved in gene regulation, including regulatory proteins, microRNAs, and the chemical modification of DNA. In particular, we discuss the current knowledge about the impact of microgravity on gene regulation in different types of bacteria, protists, fungi, animals, humans, and cells with a focus on the brain, eye, endothelium, immune system, cartilage, muscle, bone, and various cancers as well as recent findings in plants. Importantly, the obtained data clearly imply that µg experiments can support translational medicine on Earth.

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Section: Resultsmentioning

confidence: 99%

Current Knowledge about the Impact of Microgravity on Gene Regulation

Corydon

Schulz

Richter

et al. 2023

Cells

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“…Thereby, short-chained volatile acids can be analyzed by head space techniques [12], whereas the long-chained ones can be assessed by liquid injection after appropriate derivatization [13]. The latter approach, relying on detection of methyl esters, proved to be efficient in analysis of branched fatty acids in bacterial membranes [14]. However, implementation of derivatization procedures in experimental workflows dramatically reduces sample throughput and might trigger transesterification of lipids, as well as isomerization and oxidation of unsaturated FFAs [15,16].…”

Section: Figure 1 Structural Class Of Bacterial Fatty Acidsmentioning

confidence: 99%

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

Gladchuk¹,

Schumilina²,

Kusnetsova³

et al. 2020

Preprint

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Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative contents of free fatty acids (FFAs). Thereby, nitrogen-fixing bacteria (order Rhizobiales), often found in symbiosis with legumes, attract a special interest. Indeed, FAs impact on the structure of rhizobial nodulation factors, required for successful infection of plant root. Although the FA patterns can be addressed by GC- and LC-MS, these methods are time-consuming and suffer from compromised sensitivity, low stability of derivatives and artifacts. In contrast, MALDI-TOF-MS represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by the combination of Langmuir technology and MALDI-TOF-MS, which is featured with high sensitivity, accuracy and precision of quantification. Here we propose a step by step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. To demonstrate the analytical potential of the protocol we illustrate it by a case study – comparison the FFA metabolomes of two rhizobia species - Rhizobium leguminosarum and Sinorhizobium meliloti.

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“…Multiple Listeria genes associated with resisting heat ( dnaK , groES , clpC , clpP and clpE ), cold ( cspA , cspB and cspD ) and acid ( sigB ) contribute to its survival in harsh environmental conditions [ 7 ]. Once Lm enters the gastrointestinal tract, bacteria cells face several threats, such as trypsin, stomach acid, bile salts and inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Transmembrane Protein LMxysn_1693 of Serovar 4h Listeria monocytogenes Is Associated with Bile Salt Resistance and Intestinal Colonization

et al. 2022

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Listeria monocytogenes (Lm) is a ubiquitous foodborne pathogen comprising of 14 serotypes, of which serovar 4h isolates belonging to hybrid sub-lineage Ⅱ exhibit hypervirulent features. LMxysn_1693 of serovar 4h Lm XYSN, a member of genomic island-7 (GI-7), is predicted to a membrane protein with unknown function, which is conserved in serovar 4h Listeria monocytogenes. Under bile salts stress, Lm XYSN strain lacking LMxysn_1693 (XYSN∆1693) exhibited a stationary phase growth defect as well as a reduction in biofilm formation and strikingly down-regulated bile-salts-resistant genes and virulent genes. Particularly, LMxysn_1693 protein plays a crucial role in Lm XYSN adhesion and invasion to intestinal epithelial cells, as well as colonization in the ileum of mice. Taken together, these findings indicate that the LMxysn_1693 gene encodes a component of the putative ABC transporter system, synthetically interacts with genes involved in bile resistance, biofilm formation and virulence, and thus contributes to Listeria monocytogenes survival within and outside the host.

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Modulatory effect of low-shear modeled microgravity on stress resistance, membrane lipid composition, virulence, and relevant gene expression in the food-borne pathogen Listeria monocytogenes

Cited by 16 publications

References 58 publications

Current Knowledge about the Impact of Microgravity on Gene Regulation

Current Knowledge about the Impact of Microgravity on Gene Regulation

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

Transmembrane Protein LMxysn_1693 of Serovar 4h Listeria monocytogenes Is Associated with Bile Salt Resistance and Intestinal Colonization

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