Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Abstract

Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1-to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.

Comparative analysis of the relative potential of silver, Zinc-oxide and titanium-dioxide nanoparticles against UVB-induced DNA damage for the prevention of skin carcinogenesis

“…This assumption is supported by previous observations demonstrating drastically increased efficiency of enzymes after immobilization on solid surface as compared to their free forms [43]. Indeed, immobilization of amylase on the surface of Ag-NPs increased its activity by 60-90 % [44]. The enzymatic activity of glucose oxidase, cholinesterase, monoamine oxidase, etc.…”

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

“…Indeed, some studies underlined the ability of digestive enzymes to bind around NPs. [75][76][77][78][79][80] Besides, bile salts can also interact with the NPs and complete the corona. [41,81] This corona stabilizes the NPs as it was emphasized by the raise of negative zeta-potential.…”

Immobilization of Trichoderma harzianum α-amylase on PPyAgNp/Fe3O4-nanocomposite: chemical and physical properties

“…The immobilization of biomolecules on metal nanoparticles developed and improved several applications including diagnostics, biosensors and drug delivery [11][12][13][14][15][16]. Several studies reported the immobilization of a-amylase with metal nanoparticles such as magnetic Fe 3 O 4 nanoparticles [17], silica nanoparticles [18], amino-functionalized magnetite nanoparticles [19], gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) [20]. However, using high-intensity ultrasound, in situ generated a-amylase nanoparticles (NPs) were immobilized on polyethylene films [21].…”