Modulation of virulence in Acinetobacter baumannii cells surviving photodynamic treatment with toluidine blue

Pourhajibagher, Maryam; Boluki, Ebrahim; Chiniforush, Nasim; Pourakbari, Babak; Farshadzadeh, Zahra; Ghorbanzadeh, Roghayeh; Aziemzadeh, Masoud; Bahador, Abbas

doi:10.1016/j.pdpdt.2016.07.007

Cited by 53 publications

(24 citation statements)

References 54 publications

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“…A study on the effect of sub-lethal PDT (sPDT) with methylene-blue on virulence factors of A. baumanii indicates that sPDT increases the accumulation of efflux pumps and also decreases the expression of specific genes such as epsA (membrane protein responsible for polysaccharides extrusion), csuE (adhesion involved in biofilm formation), abaI (AHL-producing signal, essential for QS and biofilm development). 60 Alternatively, a recent study on V. harvey reported that low dose of laser not only is bactericidal but also has a positive effect on growth and increases QS. 61…”

Section: Antimicrobial Photodynamic Therapymentioning

confidence: 99%

The Effect of Quorum-Sensing and Efflux Pumps Interactions in Pseudomonas aeruginosa Against Photooxidative Stress

Rezaie¹,

Pourhajibagher²,

Chiniforush³

et al. 2018

J Lasers Med Sci

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Quorum SensingCommunication between similar or different species occurs via various chemical signals produced and secreted by the bacteria. These signals can be population dependent (QS) or can be secreted during several proliferation stages (e.g., indole, which is secreted in the stationary phase by Escherichia coli). AbstractResistant infections essentially cause mortality in a burn unit. Several bacteria contribute to burn infections; among these, Pseudomonas aeruginosa majorly contributes to these infections revealing significant drug resistance. Similar to other bacteria, P. aeruginosa reveals various mechanisms to attain highest pathogenicity and resistance; among these, efflux pumps and quorum sensing are crucial. Quorum sensing enables effective communication between bacteria and synchronizes their gene expression resulting in optimum effect of the secreted proteins; alternatively, efflux pumps increase the bacterial resistance by pumping out the antimicrobial factors as well as the QS signals and precursors. Of recent, increasing episodes of drug resistance led to new findings and approaches for killing pathogenic bacteria without inducing the drug-resistant species. Photodynamic therapy (PDT), considered as an adjuvant and innovative method for conventional antibiotic therapy, is a photochemical reaction that includes visible light, oxygen, and a photosensitizer (PS). In this therapy, after exposure to visible light, the PS generates reactive oxygen species (ROS) that are bacteriostatic or bactericidal. Furthermore, this oxidative stress can disrupt the coordination of gene expression and alter the bacterial behavior. Considering the fact that the adaption and several gene expression patterns of microorganisms within the biofilm make them notably resistant to the recent antimicrobial treatments, this study aimed to emphasize the relationship between the efflux pump and QS under oxidative stress and their role in P. aeruginosa's reaction to PDT.

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Section: Antimicrobial Photodynamic Therapymentioning

confidence: 99%

The Effect of Quorum-Sensing and Efflux Pumps Interactions in Pseudomonas aeruginosa Against Photooxidative Stress

Rezaie¹,

Pourhajibagher²,

Chiniforush³

et al. 2018

J Lasers Med Sci

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“…CR-XDR-AB cells surviving sAPDI showed a reduction of cell metabolic activity, increase in outer membrane permeability, and inhibition of efflux pump systems. sAPDI reduced the minimum inhibitory concentrations of the most tested antimicrobials by ≥2-fold in CR-XDR-AB strain [68]. …”

Section: Can Resistance Develop After Sub-lethal Apdi?mentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

246

173

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Infections have been a major cause of disease throughout the history of humans on earth. With the introduction of antibiotics, it was thought that infections had been conquered. However, bacteria have been able to develop resistance to antibiotics at an exponentially increasing rate. The growing threat from multi-drug resistant organisms calls for intensive action to prevent the emergence of totally resistant and untreatable infections. Novel, non-invasive, non-antibiotic strategies are needed that act more efficiently and faster than current antibiotics. One promising alternative is antimicrobial photodynamic inactivation (APDI), an approach that produces reactive oxygen species when dyes and light are combined. So far, it has been questionable if bacteria can develop resistance against APDI. This review paper gives an overview of recent studies concerning the susceptibility of bacteria towards oxidative stress, and suggests possible mechanisms of the development of APDI-resistance that should at least be addressed. Some ways to potentiate APDI and also to overcome future resistance are suggested.

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“…CR‐XDR‐AB cells surviving aPDI showed a reduction of cell metabolic activity, increase in outer membrane permeability, and inhibition of efflux pump systems. The aPDI reduced the minimum inhibitory concentrations of the most tested antimicrobials by greater than twofold in CR‐XDR‐AB strain (Pourhajibagher et al, ).…”

Section: Antimicrobial Photodynamic Inactivation (Apdi)mentioning

confidence: 99%

Can light‐based approaches overcome antimicrobial resistance?

Hamblin

Abrahamse

2018

Drug Development Research

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The relentless rise of antibiotic resistance is considered one of the most serious problems facing mankind. This mini-review will cover three cutting-edge approaches that use light-based techniques to kill antibiotic-resistant microbial species, and treat localized infections. First, we will discuss antimicrobial photodynamic inactivation using rationally designed photosensitizes combined with visible light, with the added possibility of strong potentiation by inorganic salts such as potassium iodide. Second, the use of blue and violet light alone that activates endogenous photoactive porphyrins within the microbial cells. Third, it is used for "safe UVC" at wavelengths between 200 nm and 230 nm that can kill microbial cells without damaging host mammalian cells. We have gained evidence that all these approaches can kill multidrug resistant bacteria in vitro, and they do not induce themselves any resistance, and moreover can treat animal models of localized infections caused by resistant species that can be monitored by noninvasive bioluminescence imaging. Light-based antimicrobial approaches are becoming a growing translational part of anti-infective treatments in the current age of resistance.

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Modulation of virulence in Acinetobacter baumannii cells surviving photodynamic treatment with toluidine blue

Cited by 53 publications

References 54 publications

The Effect of Quorum-Sensing and Efflux Pumps Interactions in Pseudomonas aeruginosa Against Photooxidative Stress

The Effect of Quorum-Sensing and Efflux Pumps Interactions in Pseudomonas aeruginosa Against Photooxidative Stress

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Can light‐based approaches overcome antimicrobial resistance?

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