Modulation of virulence factors of Staphylococcus aureus by nanostructured surfaces

San-Martin-Galindo, Paola; Rosqvist, Emil; Tolvanen, Stiina; Miettinen, Ilkka T.; Savijoki, Kirsi; Nyman, Tuula A.; Fallarero, Adyary; Peltonen, Jouko

doi:10.1016/j.matdes.2021.109879

Cited by 9 publications

(5 citation statements)

References 76 publications

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“…Here, surface proteomics revealed cytoplasmic proteins as the main component of the mycobacterial extracellular proteome. This is in line with a number of previous studies reporting cell surface proteomes of different bacterial species grown either as planktonic or biofilm states ( 11 , 42 - 57 ). Detection of an overwhelming number of cytoplasmic proteins, including the r-proteins, can be explained by the protein identification method used, which favors the identification of cell surface proteins that can be easily assessed by biotinylation and streptavidin capture–based technique.…”

Section: Discussionsupporting

confidence: 92%

“…Thus, instead of or in addition to structural proteins, bacteria may also use exported/released ribosomal proteins (r-proteins) as structural proteins to stabilize and strengthen the biofilm integrity, as demonstrated with Staphylococcus aureus biofilms ( 44 ). r-proteins typically form the most dominant protein group at the bacterial cell surfaces ( 45 - 58 - 51 - 57 - 58 ), which was also demonstrated in this study.…”

Section: Discussionsupporting

confidence: 79%

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In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies

et al. 2023

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The antibiotic-tolerant biofilms present in tuberculous granulomas add an additional layer of complexity when treating mycobacterial infections, including tuberculosis (TB). For a more efficient treatment of TB, the biofilm forms of mycobacteria warrant specific attention. Here, we used Mycobacterium marinum (Mmr) as a biofilm-forming model to identify the abundant proteins covering the biofilm surface. We used biotinylation/streptavidin-based proteomics on the proteins exposed at the Mmr biofilm matrices in vitro to identify 448 proteins and ex vivo proteomics to detect 91 Mmr proteins from the mycobacterial granulomas isolated from adult zebrafish. In vitro and ex vivo proteomics data are available via ProteomeXchange with identifier PXD033425 and PXD039416, respectively. Data comparisons pinpointed the molecular chaperone GroEL2 as the most abundant Mmr protein within the in vitro and ex vivo proteomes, while its paralog, GroEL1, with a known role in biofilm formation, was detected with slightly lower intensity values. To validate the surface exposure of these targets, we created in-house synthetic nanobodies (sybodies) against the two chaperones and identified sybodies that bind the mycobacterial biofilms in vitro and those present in ex vivo granulomas. Taken together, the present study reports a proof-of-concept showing that surface proteomics in vitro and ex vivo proteomics combined are a valuable strategy to identify surface-exposed proteins on the mycobacterial biofilm. Biofilm-surface-binding nanobodies could be eventually used as homing agents to deliver biofilm-targeting treatments to the sites of persistent biofilm infection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 79%

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies

et al. 2023

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“…The antibiofilm effects, bacterial adhesion, and biofilm formation, of the three different structured surfaces (micro-Cu, SHB nano-HfO 2 , and SHB nano-Cu) were tested against three clinically relevant bacterial species, involving Gram-positives S. aureus ATCC25923 (hereafter Sa_25923) and S. epidermidis (Se_RP62A), as well as Gram-negatives P. aeruginosa ATCC15442 (Pa_15442) and P. aeruginosa ATCC9027 (Pa_9027). PS having hydrophilic surface was used as the positive control of biofilm formation [30][31][32][33] and PDMS as a planar material control, since all three structured samples are composites containing PDMS. PDMS can be expected to be a nontoxic but a low surface energy, hydrophobic and a low bioadhesive surface.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Superhydrophobic Copper‐Composite Surfaces Exert Antibacterial Effects against Gram‐Negative and ‐Positive Bacteria

Mirmohammadi

Savijoki

Hoshian

et al. 2023

Adv Materials Inter

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Copper shows a high promise in developing biomedical materials with antibacterial effect. The antibacterial effect can be enhanced by nanostructured surfaces with superhydrophobic properties, which reduce the solid contact area available for bacterial adhesion and adherent growth. Here, three structured surfaces are fabricated to test the combined effect of copper and superhydrophobicity for antibacterial effects. One of the samples is superhydrophobic but does not contain copper, one contains copper but is not superhydrophobic, and the third is both superhydrophobic and contained copper. The antibiofilm and bactericidal effects of these samples are tested against medically important Gram‐positive and ‐negative bacteria including Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and Pseudomonas aeruginosa (P. aeruginosa). The findings indicate that copper alone without superhydrophobicity, while decreasing the cell viability in most of the tested species, supports remarkably more biomass compared to the reference sample. The superhydrophobic and copper bearing samples, while allowing adherent growth to take place, provide the greatest bactericidal effect against two P. aeruginosa strains, and both the antibiofilm and/or bactericidal effects against S. aureus and S. epidermidis. Thus, this study reports that nanostructured materials, combining superhydrophobicity with copper, can be the method of choice to neutralize pathogens with different cell‐wall structures and surface components mediating adherent growth.

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“…2−5 Surface topology or surface roughness often controls the adsorption/adhesion of a broad range of cell types including microbial cells and has been used widely to improve the adhesion of microbial biofilms in bioreactors. 6,7 Furthermore, surface properties such as surface energy, surface charge, and surface roughness all play critical roles in bacterial colonization of a surface. 8,9 In this study, we examine the interaction of E. coli bacteria with 21 different polymeric surfaces that have had their surface architecture modified using plasma etching.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Furthermore, the communal aspect of biofilm compounds the risk of antibiotic resistance by creating an environment that promotes the spread of antibiotic-resistant genes through processes like conjugation and transformation . Microbial biofilm formation is a multistep process that begins with the initial and perhaps most crucial step, the attachment of a planktonic bacterium to a surface. , This step is controlled by a variety of factors, and under favorable conditions, adsorbed microbes develop irreversible adhesive interactions which are followed by cellular proliferation, the secretion of extracellular materials, which leads to the formation of a mature biofilm matrix. − Surface topology or surface roughness often controls the adsorption/adhesion of a broad range of cell types including microbial cells and has been used widely to improve the adhesion of microbial biofilms in bioreactors. , Furthermore, surface properties such as surface energy, surface charge, and surface roughness all play critical roles in bacterial colonization of a surface. , …”

Section: Introductionmentioning

confidence: 99%

Escherichia coli Adhesion and Biofilm Formation on Polymeric Nanostructured Surfaces

Iyer,

Laws,

LaJeunesse

2023

ACS Omega

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Biofilm formation is a multistep process that requires initial contact between a bacterial cell and a surface substrate. Recent work has shown that nanoscale topologies impact bacterial cell viability; however, less is understood about how nanoscale surface properties impact other aspects of bacterial behavior. In this study, we examine the adhesive, viability, morphology, and colonization behavior of the bacterium Escherichia coli on 21 plasma-etched polymeric surfaces. Although we predicted that specific nanoscale surface structures of the surface would control specific aspects of bacterial behavior, we observed no correlation between any bacterial response or surface structures/properties. Instead, it appears that the surface composition of the polymer plays the most significant role in controlling and determining a bacterial response to a substrate, although changes to a polymeric surface via plasma etching alter initial bacteria colonization and morphology.

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Modulation of virulence factors of Staphylococcus aureus by nanostructured surfaces

Cited by 9 publications

References 76 publications

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies

Superhydrophobic Copper‐Composite Surfaces Exert Antibacterial Effects against Gram‐Negative and ‐Positive Bacteria

Escherichia coli Adhesion and Biofilm Formation on Polymeric Nanostructured Surfaces

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