Modulation of ultrasound-switchable fluorescence for improving signal-to-noise ratio

Kandukuri, Jayanth; Yu, Shaoming; Yao, Tingfeng; Yuan, Baohong

doi:10.1117/1.jbo.22.7.076021

Cited by 10 publications

(7 citation statements)

References 14 publications

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“…[13][14][15][16] Another technique is aimed at confining fluorescence emission only in a small volume through the interference of a FU and only switching on the fluorophores in the focal volume, so called ultrasound-switchable fluorescence (USF). [17][18][19][20][21][22][23][24][25][26][27][28] USF adopts a unique fluorescent contrast agent whose fluorescence can be switched on when temperature rises above a threshold (T th ). [19][20][21]23] When a FU beam is delivered into tissue, it induces a temperature rise in its focus.…”

Section: Introductionmentioning

confidence: 99%

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

Yu¹,

Yao²,

Liu³

et al. 2020

Biomed. Opt. Express

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Ultrasound-switchable fluorescence (USF) is a novel imaging technique that provides high spatial resolution fluorescence images in centimeter-deep biological tissue. Recently, we successfully demonstrated the feasibility of in vivo USF imaging using a frequency-domain photomultiplier tube-based system. In this work, for the first time we carried out in vivo USF imaging via a camera-based USF imaging system. The system acquires a USF signal on a two-dimensional (2D) plane, which facilitates the image acquisition because the USF scanning area can be planned based on the 2D image and provides high USF photon collection efficiency. We demonstrated in vivo USF imaging in the mouse's glioblastoma tumor with multiple targets via local injection. In addition, we designed the USF contrast agents with different particle sizes (70 nm and 330 nm) so that they could bio-distribute to various organs (spleen, liver, and kidney) via intravenous (IV) injections. The results showed that the contrast agents retained stable USF properties in tumors and some organs (spleen and liver). We successfully achieved in vivo USF imaging of the mouse's spleen and liver via IV injections. The USF imaging results were compared with the images acquired from a commercial X-ray micro computed tomography (micro-CT) system.

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Section: Introductionmentioning

confidence: 99%

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

Yu¹,

Yao²,

Liu³

et al. 2020

Biomed. Opt. Express

Self Cite

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“…During the past years, we have developed a new technology, ultrasound-switchable fluorescence (USF) imaging. What makes USF different from others is the unique contrast agent that can be switched on via ultrasound -induced thermal energy (or temperature rise) only in the ultrasound focal volume 15,[22][23][24][25] . By scanning the ultrasound focus, a 3D USF image can be acquired with fluorescence contrast and ultrasound resolution.…”

Section: Introductionmentioning

confidence: 99%

“…This feature is similar to PET imaging in which the detected  photons only come from the positrons emitted by the injected radioactive isotope 27 . In addition, USF signal strength and dynamic pattern can be externally manipulated by controlling the ultrasound exposure, which makes the USF signal uniquely differentiated from the noise to achieve high signal-to-noise ratio (SNR) or sensitivity [23][24] . Besides structural, functional and molecular imaging, USF can be seamlessly developed for high-intensity focused ultrasound (HIFU) treatment as a tool predicting pre-treatment, monitoring intro-treatment and evaluating post-treatment.…”

Section: Introductionmentioning

confidence: 99%

In vivo ultrasound-switchable fluorescence imaging

Yao

Liu

et al. 2019

Sci Rep

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The conventional fluorescence imaging has limited spatial resolution in centimeter-deep tissue because of the tissue’s high scattering property. Ultrasound-switchable fluorescence (USF) imaging, a new imaging technique, was recently proposed to realize high-resolution fluorescence imaging in centimeter-deep tissue. However, in vivo USF imaging has not been achieved so far because of the lack of stable near-infrared contrast agents in a biological environment and the lack of data about their biodistributions. In this study, for the first time, we achieved in vivo USF imaging successfully in mice with high resolution. USF imaging in porcine heart tissue and mouse breast tumor via local injections were studied and demonstrated. In vivo and ex vivo USF imaging of the mouse spleen via intravenous injections was also successfully achieved. The results showed that the USF contrast agent adopted in this study was very stable in a biological environment, and it was mainly accumulated into the spleen of the mice. By comparing the results of CT imaging and the results of USF imaging, the accuracy of USF imaging was proved.

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“…Second, USF has high detection sensitivity because we have developed several USF contrast agents that are highly sensitive to a small temperature change (only a few Celsius degrees) and could release strong fluorescence after being switched on 17,23–26 . Also, USF signal can be externally manipulated by controlling the FU signal and the data acquisition time 27 in a sensitive USF imaging system, which makes USF achieve a desired signal-to-noise ratio (SNR). Third, since USF only requires a temperature increase of a few Celsius degrees in tissue, it is safe for in vivo imaging.…”

Section: Introductionmentioning

confidence: 99%

An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system

Yao

Yuan

2019

Sci Rep

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Fluorescence imaging in centimeter-deep tissues with high resolution is highly desirable for many biomedical applications. Recently, we have developed a new imaging modality, ultrasound-switchable fluorescence (USF) imaging, for achieving this goal. In our previous work, we successfully achieved USF imaging with several types of USF contrast agents and imaging systems. In this study, we introduced a new USF imaging system: an intensified charge-coupled device (ICCD) camera-based, time-domain USF imaging system. We demonstrated the principle of time-domain USF imaging by using two USF contrast agents. With a series of USF imaging experiments, we demonstrated the tradeoffs among different experimental parameters (i.e., data acquisition time, including CCD camera recording time and intensifier gate delay; focused ultrasound (FU) power; and imaging depth) and the image qualities (i.e., signal-to-noise ratio, spatial resolution, and temporal resolution). In this study, we also discussed several imaging strategies for achieving a high-quality USF image via this time-domain system.

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Modulation of ultrasound-switchable fluorescence for improving signal-to-noise ratio

Cited by 10 publications

References 14 publications

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

In vivo ultrasound-switchable fluorescence imaging using a camera-based system

In vivo ultrasound-switchable fluorescence imaging

An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system

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