Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N‐1 perfusion seed culture

Karst, Daniel J.; Ramer, Kevin; Hughes, Erik H.; Jiang, Canping; Jacobs, Pieter J.; Mitchelson, Fernie G.

doi:10.1002/btpr.3040

Cited by 5 publications

(2 citation statements)

References 32 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tangential flow filtration provides a solution for smaller microalgae. It can somewhat alleviate the issue of filter fouling, as the medium flows tangentially across the membrane, maintaining the cells in suspension [ 60 ]. Filter membrane replacement and pumping are the main expenses associated with filtration.…”

Section: Microalgae Harvesting: Exploring Suitable Strategy For Bulk ...mentioning

confidence: 99%

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

Qin,

Wang,

Gao

et al. 2023

Biotechnol Biofuels

View full text Add to dashboard Cite

Microalgal biomass represents a sustainable bioresource for various applications, such as food, nutraceuticals, pharmaceuticals, feed, and other bio-based products. For decades, its mass production has attracted widespread attention and interest. The process of microalgal biomass production involves several techniques, mainly cultivation, harvesting, drying, and pollution control. These techniques are often designed and optimized to meet optimal growth conditions for microalgae and to produce high-quality biomass at acceptable cost. Importantly, mass production techniques are important for producing a commercial product in sufficient amounts. However, it should not be overlooked that microalgal biotechnology still faces challenges, in particular the high cost of production, the lack of knowledge about biological contaminants and the challenge of loss of active ingredients during biomass production. These issues involve the research and development of low-cost, standardized, industrial-scale production equipment and the optimization of production processes, as well as the urgent need to increase the research on biological contaminants and microalgal active ingredients. This review systematically examines the global development of microalgal biotechnology for biomass production, with emphasis on the techniques of cultivation, harvesting, drying and control of biological contaminants, and discusses the challenges and strategies to further improve quality and reduce costs. Moreover, the current status of biomass production of some biotechnologically important species has been summarized, and the importance of improving microalgae-related standards for their commercial applications is noted.

show abstract

Section: Microalgae Harvesting: Exploring Suitable Strategy For Bulk ...mentioning

confidence: 99%

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

Qin,

Wang,

Gao

et al. 2023

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…The effect of TMP is a complex and sensitive issue, and a ltration system could have a critical TMP condition in many cases. [30][31][32] However, this ltration system was operated at TMP in the range under 1 kPa because the inlet ow was modulated at low-level pressure (Fig. S3b and c †).…”

Section: Paper Nanoscale Advancesmentioning

confidence: 99%

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

et al. 2023

View full text Add to dashboard Cite

show abstract

Evaluation of various membranes at different fluxes to enable large‐volume single‐use perfusion bioreactors

Raza,

Tang,

Gao

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

The growing demand for biological therapeutics has increased interest in large‐volume perfusion bioreactors, but the operation and scalability of perfusion membranes remain a challenge. This study evaluates perfusion cell culture performance and monoclonal antibody (mAb) productivity at various membrane fluxes (1.5–5 LMH), utilizing polyvinylidene difluoride (PVDF), polyethersulfone (PES), or polysulfone (PS) membranes in tangential flow filtration mode. At low flux, culture with PVDF membrane maintained higher cell culture growth, permeate titer (1.06–1.34 g/L) and sieving coefficients (≥83%) but showed lower permeate volumetric throughput and higher transmembrane pressure (TMP) (>1.50 psi) in the later part of the run compared to cultures with PES and PS membrane. However, as permeate flux increased, the total mass of product decreased by around 30% for cultures with PVDF membrane, while it remained consistent with PES and PS membrane, and at the highest flux studied, PES membrane generated 12% more product than PVDF membrane. This highlights that membrane selection for large‐volume perfusion bioreactors depends on the productivity and permeate flux required. Since operating large‐volume perfusion bioreactors at low flux would require several cell retention devices and a complex setup, PVDF membranes are suitable for low‐volume operations at low fluxes whereas PES membranes can be a desirable alternative for large‐volume higher demand products at higher fluxes.

show abstract

Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N‐1 perfusion seed culture

Cited by 5 publications

References 32 publications

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Evaluation of various membranes at different fluxes to enable large‐volume single‐use perfusion bioreactors

Contact Info

Product

Resources

About