Modulation of TNF-α, IL-1Ra and IFN-γ in equine whole blood culture by glucocorticoids

Schrödl, Wieland; Abraham, Getu

doi:10.1016/j.vetimm.2019.03.002

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…In equine whole blood cultures, dexamethasone, and to a lesser extent hydrocortisone, inhibits the stimulated release of TNF‐α, IL‐1Ra, and IFN‐γ. 85 Triamcinolone (0.04 mg/kg IV once) administered to healthy horse decreases the production of several eicosanoids (thromboxane B 2 , prostaglandin E 2 and prostaglandin F 2α ) in whole blood stimulated by LPS. 86 Finally, dexamethasone (10 −5 M) decreases the gene expression of CXCL‐8 induced by IL‐4, LPS, and TNF‐α from pulmonary artery endothelial cells of normal adult horses.…”

Section: Effects Of Gc On Inflammationmentioning

confidence: 99%

Glucocorticoid treatment in horses with asthma: A narrative review

Mainguy-Seers

Lavoie

2021

Veterinary Internal Medicne

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Despite substantial research efforts to improve the treatment and outcome of horses with asthma, glucocorticoids (GC) remain the cornerstone of drug treatment of this prevalent disease. The high efficacy of GC to relieve airway obstruction explains their extensive use despite potential deleterious effects. However, much is yet to be uncovered concerning GC use in horses with asthma, including the comparative efficacy of the different drugs, the determination of minimal effective doses and the mechanisms underlying their variable modulation of airway inflammation. The objectives of this structured review were to report and compare the plethora of effects of the various GC used in asthmatic horses with a focus on impact on lung function, airway inflammation, and bronchial remodeling. Adverse effects are also briefly described, with an emphasis on those that have been specifically reported in horses with asthma. Ultimately, we aimed to highlight gaps in the literature and to identify future research areas.

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Section: Effects Of Gc On Inflammationmentioning

confidence: 99%

Glucocorticoid treatment in horses with asthma: A narrative review

Mainguy-Seers

Lavoie

2021

Veterinary Internal Medicne

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“…Blood samples were 1:1 diluted in sterile phosphate buffered saline (PBS) containing 2 mM EDTA, and after layered over Ficoll ® Paque Plus (GE Healthcare), according to manufacturer protocols) PBMCs from the Ficoll layer were washed twice in PBS/EDTA. PBMCs were re-suspended in RPMI-1640 medium (Sigma-Aldrich, Milano, Italy) containing penicillin G (100 U/mL) (Sigma-Aldrich, Milano, Italy), streptomycin (100 µg/mL) (Sigma-Aldrich, Milano, Italy), heparin (10 U/mL) (Sigma-Aldrich, Milano, Italy) and 10% horse serum, according on what has seen previously with slight modification [17].…”

Section: Peripheral Blood Mononuclear Cell (Pbmc) Isolationmentioning

confidence: 99%

Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study

Gugliandolo

Crupi

Biondi

et al. 2020

Animals

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Although inflammation is an important physiological response, it plays a prominent role in several diseases across the mammalian species. In horses, in particular, inflammation secondary to bacterial infection or translocation is one of the most frequent causes of morbidity and mortality. Research in new molecules with anti-inflammatory and immunomodulatory proprieties and safe use profile is constantly an active field; natural compounds are an important source of molecules with peculiar properties such as antioxidants, anti-inflammatory and immune modulating. Silibinin, a natural polyphenolic flavonoid, extracted from plant milk thistle, Silybum marianum, has been reported to have actions such as antioxidant immunomodulatory and anti-inflammatory. The aim of this study was to test the effect of silibinin on lipopolysaccharide (LPS)-induced inflammatory response in equine peripheral blood mononuclear cells (PBMCs). Our results showed the protective effect of silibinin 10 μM and 50 μM in equine PBMCs stimulated with LPS. Silibilinin was able to prevent the LPS induced increased levels of TNF-α, IL-1β, IL-6 and IL-8. The results from this study on LPS-stimulated equine PBMCs showed that silibinin could be a useful pharmacological approach in treatment or prevention of several inflammatory conditions in horse.

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Promising Therapy for Heart Failure in Patients with Severe COVID-19: Calming the Cytokine Storm

Xiang

Wang

et al. 2021

Cardiovasc Drugs Ther

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The coronavirus disease 19 (COVID-19) pandemic poses a serious global threat to human health and the economy. Based on accumulating evidence, its continuous progression involves not only pulmonary injury but also damage to the cardiovascular system due to intertwined pathophysiological risks. As a point of convergence in the pathophysiologic process between COVID-19 and heart failure (HF), cytokine storm induces the progression of COVID-19 in patients presenting pre-existing or new onset myocardial damage and even HF. Cytokine storm, as a trigger of the progression of HF in patients with COVID-19, has become a novel focus to explore therapies for target populations. In this review, we briefly introduce the basis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and illuminate the mechanism and links among COVID-19, cytokine storm, and HF. Furthermore, we discuss drugs and therapeutic targets for patients with COVID-19 and HF.

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Modulation of TNF-α, IL-1Ra and IFN-γ in equine whole blood culture by glucocorticoids

Cited by 7 publications

References 40 publications

Glucocorticoid treatment in horses with asthma: A narrative review

Glucocorticoid treatment in horses with asthma: A narrative review

Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study

Promising Therapy for Heart Failure in Patients with Severe COVID-19: Calming the Cytokine Storm

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