Modulation of thermal stress response by prostaglandins in gills of the blue mussel Mytilus edulis

Duran-Encinas, Yazmin; Tremblay, Réjean; Genard, Bertrand; Rivera-Pérez, Crisalejandra; Lora-Vilchis, María Concepción; Kraffe, Edouard; Palacios, Elena

doi:10.1016/j.aquaculture.2023.740478

Cited by 2 publications

(1 citation statement)

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“…Therefore, we proposed that PEPCKs maintained a certain level of expression in scallops and had separate activity levels adjusted to meet physiological demands. Studies showed that the striated muscle was an important motor and feeding organ and the gill was a vital respiratory organ whose changes under stress had crucial effects on mollusks [68,69]. Therefore, the high expression levels of CfPEPCK1 in striated muscle and gill suggested its potential significance in the regulation of movement metabolism and stress response.…”

Section: Glucose-succinate Pathwaymentioning

confidence: 99%

The Glucose–Succinate Pathway: A Crucial Anaerobic Metabolic Pathway in the Scallop Chlamys farreri Experiencing Heat Stress

Bao,

Liu,

Sui

et al. 2024

IJMS

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Recently, the increase in marine temperatures has become an important global marine environmental issue. The ability of energy supply in marine animals plays a crucial role in avoiding the stress of elevated temperatures. The investigation into anaerobic metabolism, an essential mechanism for regulating energy provision under heat stress, is limited in mollusks. In this study, key enzymes of four anaerobic metabolic pathways were identified in the genome of scallop Chlamys farreri, respectively including five opine dehydrogenases (CfOpDHs), two aspartate aminotransferases (CfASTs) divided into cytoplasmic (CfAST1) and mitochondrial subtype (CfAST2), and two phosphoenolpyruvate carboxykinases (CfPEPCKs) divided into a primitive type (CfPEPCK2) and a cytoplasmic subtype (CfPEPCK1). It was surprising that lactate dehydrogenase (LDH), a key enzyme in the anaerobic metabolism of the glucose–lactate pathway in vertebrates, was absent in the genome of scallops. Phylogenetic analysis verified that CfOpDHs clustered according to the phylogenetic relationships of the organisms rather than substrate specificity. Furthermore, CfOpDHs, CfASTs, and CfPEPCKs displayed distinct expression patterns throughout the developmental process and showed a prominent expression in muscle, foot, kidney, male gonad, and ganglia tissues. Notably, CfASTs displayed the highest level of expression among these genes during the developmental process and in adult tissues. Under heat stress, the expression of CfASTs exhibited a general downregulation trend in the six tissues examined. The expression of CfOpDHs also displayed a downregulation trend in most tissues, except CfOpDH1/3 in striated muscle showing significant up-regulation at some time points. Remarkably, CfPEPCK1 was significantly upregulated in all six tested tissues at almost all time points. Therefore, we speculated that the glucose–succinate pathway, catalyzed by CfPEPCK1, serves as the primary anaerobic metabolic pathway in mollusks experiencing heat stress, with CfOpDH3 catalyzing the glucose–opine pathway in striated muscle as supplementary. Additionally, the high and stable expression level of CfASTs is crucial for the maintenance of the essential functions of aspartate aminotransferase (AST). This study provides a comprehensive and systematic analysis of the key enzymes involved in anaerobic metabolism pathways, which holds significant importance in understanding the mechanism of energy supply in mollusks.

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Section: Glucose-succinate Pathwaymentioning

confidence: 99%