Modulation of the mRNA of the Nlrp3 inflammasome by Morin and PUFAs in an obesity model induced by a high-fat diet

López-Tenorio, Itzel Ivonn; Domínguez‐López, Aarón; Miliar-García, Ángel; Escalona-Cardoso, Gerardo; Real-Sandoval, Samantha Alejandra; Gómez-Alcalá, Alejandro; Jaramillo-Flores, M. E.

doi:10.1016/j.foodres.2020.109706

Cited by 13 publications

(19 citation statements)

References 25 publications

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“…Moreover, the PPARα activation positively affects fatty acid catabolism improving lipid profile in liver. A similar effect, albeit to a lesser extent, has been observed in the same experimental model after treatment with a mixture of EPA and DHA (eicosapentaenoic and docosaexaenoic acid, respectively) [88] The efficacy of rosiglitazone in reducing inflammation via modulation of NLRP3 has been more recently confirmed in a model of radiation-induced acute intestinal injury and in intestinal macrophages isolated from the same animals [69].…”

Section: Ppars Inflammation and Oxidative Stresssupporting

confidence: 60%

Peroxisome Proliferator-Activated Receptors (PPARs) and Oxidative Stress in Physiological Conditions and in Cancer

2021

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Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear hormone receptor superfamily. Originally described as “orphan nuclear receptors”, they can bind both natural and synthetic ligands acting as agonists or antagonists. In humans three subtypes, PPARα, β/δ, γ, are encoded by different genes, show tissue-specific expression patterns, and contribute to the regulation of lipid and carbohydrate metabolisms, of different cell functions, including proliferation, death, differentiation, and of processes, as inflammation, angiogenesis, immune response. The PPAR ability in increasing the expression of various antioxidant genes and decreasing the synthesis of pro-inflammatory mediators, makes them be considered among the most important regulators of the cellular response to oxidative stress conditions. Based on the multiplicity of physiological effects, PPAR involvement in cancer development and progression has attracted great scientific interest with the aim to describe changes occurring in their expression in cancer cells, and to investigate the correlation with some characteristics of cancer phenotype, including increased proliferation, decreased susceptibility to apoptosis, malignancy degree and onset of resistance to anticancer drugs. This review focuses on mechanisms underlying the antioxidant and anti-inflammatory properties of PPARs in physiological conditions, and on the reported beneficial effects of PPAR activation in cancer.

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Section: Ppars Inflammation and Oxidative Stresssupporting

confidence: 60%

Peroxisome Proliferator-Activated Receptors (PPARs) and Oxidative Stress in Physiological Conditions and in Cancer

2021

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“…In this study, we administered PUFA n3 immediately and every 24 h for the next three days after the operation to induce its neuroprotective and anti-inflammatory effects. There are few studies that describe the effect of omega-3 on inflammasome regulation in other animal models [15,29]. It was demonstrated that PUFA n3 suppresses inflammation by reducing NLRP3 inflammasome activation in activated macrophages [30].…”

Section: Discussionmentioning

confidence: 99%

Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model

Baazm

Behrens

Beyer

et al. 2021

Cells

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Omega-3 polyunsaturated fatty acids (PUFA n3) ameliorate inflammation in different diseases and potentially improve neurological function after neuronal injury. Following spinal cord injury (SCI), inflammatory events result in caspase-1 mediated activation of interleukin-1 beta (IL-1b) and 18. We aim to evaluate the neuroprotective potency of PUFA n3 in suppressing the formation and activation of inflammasomes following SCI. Male Wistar rats were divided into four groups: control, SCI, SCI+PUFA n3, and SCI+Lipofundin MCT (medium-chain triglyceride; vehicle). PUFA n3 or vehicle was intravenously administered immediately after SCI and every 24 h for the next three days. We analyzed the expression of NLRP3, NLRP1, ASC, caspase-1, IL-1b, and 18 in the spinal cord. The distribution of microglia, oligodendrocytes, and astrocytes was assessed by immunohistochemistry analysis. Behavioral testing showed significantly improved locomotor recovery in PUFA n3-treated animals and the SCI-induced upregulation of inflammasome components was reduced. Histopathological evaluation confirmed the suppression of microgliosis, increased numbers of oligodendrocytes, and the prevention of demyelination by PUFA n3. Our data support the neuroprotective role of PUFA n3 by targeting the NLRP3 inflammasome. These findings provide evidence that PUFA n3 has therapeutic effects which potentially attenuate neuronal damage in SCI and possibly also in other neuronal injuries.

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“…In turn, low expression of TLR4 by hepatic dendritic cells may contribute to the reduced or altered activation of hepatic adaptive immune responses [59]. Previous studies have shown HFD-stimulated hepatic infiltration of macrophages in LDLR-KO mice, but these effects were abolished by TLR4 deficiency and decreasing the expression of important components of the NLRP3 inflammasome [19,82]. Thus, liver integrity and inflammatory processes might be associated with a decrease in the expression of TLR4 and Nod signaling-related genes [83].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, obesity-associated DAMPs also activate multiprotein complexes through NLRs called inflammasomes, and the NLR family pyrin domain-containing 3 (NLRP3) inflammasome is the best characterized inflammasome [19]. Inflammasome activation leads to the cleavage, activation, and secretion of several proinflammatory cytokines, resulting in the modulation of the immune response [8].…”

Section: Introductionmentioning

confidence: 99%

“…Liver inflammation and ROS generation are also heightened by metabolic alterations in the β-oxidation pathway and fatty acid synthesis [22], whose balance is regulated by peroxisome proliferator-activated receptor α (PPARα), a transcription factor located in the nucleus that induces the expression of various genes involved in the β-oxidation pathway and is downregulated in NAFLD [19].…”

Section: Introductionmentioning

confidence: 99%

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White Button Mushroom Extracts Modulate Hepatic Fibrosis Progression, Inflammation, and Oxidative Stress In Vitro and in LDLR-/- Mice

Gallego

Luque-Sierra

Falcón

et al. 2021

Foods

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Liver fibrosis can be caused by non-alcoholic steatohepatitis (NASH), among other conditions. We performed a study to analyze the effects of a nontoxic, water-soluble extract of the edible mushroom Agaricus bisporus (AB) as a potential inhibitor of fibrosis progression in vitro using human hepatic stellate cell (LX2) cultures and in vivo in LDLR-/- mice. Treatment of LX2 cells with the AB extract reduced the levels of fibrotic and oxidative-related markers and increased the levels of GATA4 expression. In LDLR-/- mice with high-fat diet (HFD)-induced liver fibrosis and inflammation, the progression of fibrosis, oxidative stress, inflammation, and apoptosis were prevented by AB extract treatment. Moreover, in the mouse model, AB extract could exert an antiatherogenic effect. These data suggest that AB mushroom extract seems to exert protective effects by alleviating inflammation and oxidative stress during the progression of liver fibrosis, possibly due to a decrease in Toll-like receptor 4 (TLR4) expression and a reduction in Nod-like receptor protein 3 (NLRP3) inflammasome activation. In addition, we observed a potential atheroprotective effect in our mouse model.

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Modulation of the mRNA of the Nlrp3 inflammasome by Morin and PUFAs in an obesity model induced by a high-fat diet

Cited by 13 publications

References 25 publications

Peroxisome Proliferator-Activated Receptors (PPARs) and Oxidative Stress in Physiological Conditions and in Cancer

Peroxisome Proliferator-Activated Receptors (PPARs) and Oxidative Stress in Physiological Conditions and in Cancer

Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model

White Button Mushroom Extracts Modulate Hepatic Fibrosis Progression, Inflammation, and Oxidative Stress In Vitro and in LDLR-/- Mice

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