Modulation of the Metastatic Activity of Melanoma Cells by Laminin and Fibronectin

Terranova, Victor P.; Williams, Jeannette E.; Liotta, Lance A.; Martin, George R.

doi:10.1126/science.6505678

Cited by 228 publications

(110 citation statements)

References 39 publications

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“…The adhesive interaction between tumour cells and/or platelets may form homotypic or heterotypic cell clumps and aggregations which can subsequently be arrested and extravasated (Terranova et al, 1982(Terranova et al, , 1984. Such interactions may also lead to an enhancement and stabilisation of tumour cell arrest in capillary vessels by increasing the size of tumour cell emboli as well as by shielding the tumour cells from immune response.…”

Section: Discussionmentioning

confidence: 99%

“…As a result of adhesive interaction, this encounter may lead to a multicellular embolus formation that includes homotypic or heterotypic cell clumps which can subsequently enhance the survival, arrest and invasiveness of tumour cells (Terranova et al, 1982(Terranova et al, , 1984. Such specific interaction is therefore a fundamental event in the metastatic process.…”

mentioning

confidence: 99%

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Antimetastatic effects of synthetic polypeptides containing repeated structures of the cell adhesive Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) sequences

Saiki

Murata²,

Iida³

et al. 1989

Br J Cancer

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Summary We have investigated the inhibitory effect on experimental or spontaneous lung metastases of polypeptides which contain repetitive structures of the Arg-Gly-Asp (RGD) or Tyr-Ile-Gly-Ser-Arg (YIGSR) sequence derived from adhesion molecules, and studied their biological characterisation after administration. In the spontaneous metastasis model, multiple intravenous (i.v.) administrations of poly (RGD) and poly (YIGSR) resulted in a reduction of lung tumour colonies, although the monomer peptides, RGD or YIGSR, had no effect under these conditions. The treatment with poly(RGD) substantially prolonged the survival time for mice injected i.v. with B16-BL6 cells as compared to the treatment with RGD and random poly(R, G, D). Tumour cell adhesion to the fibronectin-substrates was remarkably inhibited by adding poly(RGD) freely in solution. Poly(RGD) was found to inhibit completely the ability of platelets to enhance tumour cell adhesion to fibronectin-substrate and tumour cell-elicited platelet aggregation in vitro, but poly(R, G, D) had no such effect. We also found that poly(RGD) led to a decrease in the arrest and retention of tumour cells after its co-injection with radiolabelled tumour cells and that the radiolabelled polypeptide can be at least decomposed into small fragments during circulation. Poly(RGD) was found to be still active in inhibiting experimental lung metastasis even when the contributions of NK cells or macrophages were removed from this system after pretreatment with anti-asialo GM1 serum, 2-chloroadenosine or carrageenan. The results indicate that the poly(RGD)-mediated inhibition of tumour metastasis may be due to the interference of the adhesive interaction of tumour cells with a specific site in the target organs. Derivatives of polypeptides which contain RGD and/or YIGSR sequences derived from cell adhesion proteins may thus provide a promising approach for the control and prevention of cancer metastasis.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Antimetastatic effects of synthetic polypeptides containing repeated structures of the cell adhesive Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) sequences

Saiki

Murata²,

Iida³

et al. 1989

Br J Cancer

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“…[97][98][99][100] However, tumor metastases were increased when cells were co-injected with laminin into blood vessels. [101][102][103] Interestingly, the active site responsible for the increase in metastatic potential is located in a region of the laminin molecule different from the inhibition site YIGSR. 102 …”

Section: Metastasismentioning

confidence: 99%

Involvement of extracellular matrix constituents in breast cancer

Lochter

Bissell

1995

Seminars in Cancer Biology

164

136

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“…Metastatic tumor cells attach preferentially to laminin, the predominant glycoprotein in basement membranes (5,6). Laminin stimulates tumor cell adhesion (7), collagenase IV production (8), cell motility (9,10), and the formation of metastasis (6).…”

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confidence: 99%

Reconstituted basement membrane (matrigel) and laminin can enhance the tumorigenicity and the drug resistance of small cell lung cancer cell lines.

Fridman

Giaccone

Kanemoto

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

219

157

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Small cell lung cancer (SCLC) is a fatal malignancy due to its propensity to metastasize widely and to reoccur after chemotherapy in a drug-resistant form. While most SCLC cell lines are anchorage independent for growth, laminin induced the attachment of five of six SCLC cell lines tested (NCI-N417, NCI-H345, NCI-H146, NCI-H187, NCI-H510, and NCI-H209 These observations suggest that the interaction of cells with laminin as well as with other basement membrane components may play a role in tumor growth and drug resistance.MATERIALS AND METHODS Extracellular Matrix Proteins, Matrigel, and Synthetic Peptides. Laminin, collagen IV, heparan sulfate proteoglycan, and matrigel were all isolated from the EHS tumor as described (12, 13) and were used as substrates in vitro in standard attachment assays as described (5, 6). Human fibronectin and bovine collagen I were purchased from Collaborative Research. Cyclic peptides were synthesized as described (14) with an automated synthesizer model 930A (Applied Biosystems), and their purity was ascertained by amino acid analyses and by high-performance liquid chromatography. The synthetic peptides were filtered to remove contaminating endotoxin (14).Cell Culture. SCLC cell lines (NCI-N417, NCI-H187, NCI-146, NCI-H209, NCI-510) were cultured in RPMI 1640 medium (GIBCO) supplemented with 10o fetal calf serum, glutamine (2 mM), penicillin (100 units/ml), streptomycin (100 ,g/ml), gentamicin (50 ,ug/ml), and Hepes buffer (25 mM) and were all purchased from GIBCO. NCI-H345 cells were grown in serum-free medium, HITES, as described (15).Cell Motility Assay. Cell migration was tested by using modified Boyden chambers with a polyvinylpyrrolidone-free (pore size, 8 ,um) polycarbonate filter (Neuroprobe, Cabin John, MD) placed over the lower well previously filled with RPMI 1640 medium (0.22 ml) containing 0.1% bovine serum albumin and increasing concentrations of laminin. Alternatively, laminin was dried directly on the lower surface of the filters. A suspension of SCLC cells (2 x 105 cells in 0.85 ml of RPMI 1640 medium/0.1% bovine serum albumin) was added to the upper well. After a 5-hr incubation at 37°C in 5% C02/95% air, the filters were removed, fixed, and stained with Diff-Quick stain (American Scientific Products, McGaw Park, IL). The area occupied by the cells that migrated to the lower surface of the filters was quantitated by image analysis (Optomax IV, Analytical Measuring Systems, Essex, U.K.).In Vivo Tumor Studies. Cultured SCLC cells were harvested by centrifugation (5 min at 1000 rpm), the cell clumps were disaggregated by gentle trituration, and an aliquot ofthe suspension was assessed for cell number and viability. Cells were resuspended in cold serum-free RPMI 1640 medium and mixed with either an equal volume of cold liquid matrigel (10 mg/ml), laminin (2 mg/ml), or collagen I (3 mg/ml, previously neutralized), and a final vol of 0.5 ml was immediately Abbreviation: SCLC, small cell lung cancer.

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Modulation of the Metastatic Activity of Melanoma Cells by Laminin and Fibronectin

Cited by 228 publications

References 39 publications

Antimetastatic effects of synthetic polypeptides containing repeated structures of the cell adhesive Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) sequences

Antimetastatic effects of synthetic polypeptides containing repeated structures of the cell adhesive Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) sequences

Involvement of extracellular matrix constituents in breast cancer

Reconstituted basement membrane (matrigel) and laminin can enhance the tumorigenicity and the drug resistance of small cell lung cancer cell lines.

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