Modulation of the levels of interleukin-8 messenger ribonucleic acid and interleukin-8 protein synthesis in human endometrial stromal cells by transforming growth factor-beta 1

Arici, A.

doi:10.1210/jc.81.8.3004

Cited by 12 publications

(8 citation statements)

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“…In endometrial cells, the level of IL-8 messenger RNA can be increased by TGF␤ (37). It has been found in this study that TGF␤1 inhibits the expression of IL-8 receptor type B (IL-8RB) in isolated myometrial cells.…”

Section: Discussionmentioning

confidence: 67%

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Complex Interactions Between Sex Steroids and Cytokines in the Human Pregnant Myometrium: Evidence for an Autocrine Signaling System at Term*

Hatthachote

Gillespie

1999

Endocrinology

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Little is known about the mechanisms controlling the expression of key proteins that regulate excitability and contractility in the human myometrium at term. However, evidence is accumulating to suggest that the cytokine transforming growth factor (TGF)beta may play a central role. TGFbeta1 and TGFbeta receptors are present in the myometrial cells, indicative of an autocrine signaling system. Furthermore, the levels of TGFbeta1 and the expression of its receptors increase in the myometrium at term suggesting that they are, in turn, regulated and form part of a physiological cascade of events involving a number of autocrine signaling associated proteins. The present experiments were done to identify factors that regulate the expression of TGFbeta1 and TGFbeta receptors and may form other elements of this cascade. Because IL-1 and IL-8 are found in the myometrium at term and have been implicated in the etiology in premature labor we focus on this cytokines. Receptors for IL-1 and IL-8 were detected in the myometrial cells. Using Western blot analysis, the levels of expression were found to vary. The expression of IL-1 receptor type I was highest in the nonpregnant tissue with lower levels in nonlaboring myometrium with a further reduction in the spontaneously laboring tissue. In contrast, the expression of IL-8 receptor type B was highest in the pregnant nonlaboring tissue with a lower level in the spontaneously laboring tissue. Using an in vitro model, TGFbeta1 and TGFbeta receptor expression was up-regulated by IL-8, IL-1, and TGFbeta1 itself. However, IL-8 receptor expression was decreased by IL-8 and TGFbeta1. This suggests that in a cascade IL-8 would feed forward to promote the TGFbeta system, whereas TGFbeta1 feeds back to inhibit responsiveness to IL-8. Estrogen and progesterone increased the release of TGFbeta1. However, at high concentrations, estrogen and progesterone (100 nM 17beta-estradiol or 200 nM progesterone) decreased the level of TGFbeta receptor expression. Thus, the progressive rise of steroid levels in vivo might account for the observed changes in TGFbeta1 and TGFbeta receptor expression in vivo. Taken together, these observations support the idea that there is a cascade of autocrine signals that may play a major role in the physiological processes preparing the myometrium for parturition at term.

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Section: Discussionmentioning

confidence: 67%

“…In human endometrial cells, TGF␤1 acts to potentiate the effect of IL-1␣ to increase the level of IL-8 gene expression (37). There is evidence that IL-1 and TNF␣ can regulate the level of IL-8 production in human endometrial cells in culture (39) and in human hepatoma cells (40).…”

Section: Discussionmentioning

confidence: 99%

Complex Interactions Between Sex Steroids and Cytokines in the Human Pregnant Myometrium: Evidence for an Autocrine Signaling System at Term*

Hatthachote

Gillespie

1999

Endocrinology

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“…L'IL-4 stimule la production du « leukemia inhibiting factor » (LIF) par le tissu endométrial, ce qui favorise avec le TGFb la décidualisation de l'endomètre, l'invasion trophoblastique et le contrôle de l'angiogenèse au niveau des villosités trophoblastiques [33][34][35][36][37][38]. Le rôle de la progestérone est majeur [33,36,39].…”

Section: Profil Cytokinique Gestationnelunclassified

Immunologie de la grossesse

Kayem

Batteux

2008

La Presse Médicale

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“…Inducers of IL-8 in gestational tissues include: IL-ß, TNF, TGF-ß1, [100]; mechanical stretch [96], and hyaluronic acid [101]. IL-8 is inhibited by progesterone and dexamethasone and stimulated by mifepristone [102].…”

Section: Role In Parturitionmentioning

confidence: 99%

Cytokines and the Initiation of Parturition

Rice

2001

Frontiers of Hormone Research

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Rice 114 the inflammatory response. Most cytokines are pleiotropic, displaying functional plasticity and redundancy. They are involved in extensive, complex networks and may act synergistically as well as antagonistically in regulating target cell function. Human parturition displays many of the hallmarks of an inflammatory response, thus, the aim of this review is to consider the data available implicating cytokines in the initiation and/or progression of human parturition. To assess the putative involvement of cytokines in the process of human parturition an appreciation of their general biology is a requisite.Cytokines are low molecular weight polypeptides involved in the regulation of cell growth, activation and cell repair, immune responses, inflammation, hemopoiesis and fibrosis. Proteins classified as cytokines include interleukins (ILs), tumor necrosis factors (TNFs), interferons (IFNs), colony-stimulating factors (CSFs), erythropoietin, thrombopoietin and various other growth factors. This review focuses on the ILs and TNFs and their putative role in the proximal events involved in the initiation and/or progression of parturition. Interleukin-1: 2q14Structure Two functional forms of IL-1 (aka endogenous pyrogen, lymphocyte-activating factor), IL-1· (17 kD, 159 amino acids, pI = 5.0) and IL-1ß (17 kD, 153 amino acids, pI = 7.0) that are encoded by two different genes on chromosome 2 have been identified. The · and ß forms display approximately 27% homology, mainly restricted to the carboxy-terminal region, and their effects are mediated via the same receptor. IL-1s are synthesized as precursor molecules that are proteolytically cleaved to release mature peptides. The IL-1ß precursor can be cleaved by proteases including caspase-1, elastase, cathepsin G, and collagenase. ExpressionCells known to express IL-1· include:

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Modulation of the levels of interleukin-8 messenger ribonucleic acid and interleukin-8 protein synthesis in human endometrial stromal cells by transforming growth factor-beta 1

Cited by 12 publications

References 0 publications

Complex Interactions Between Sex Steroids and Cytokines in the Human Pregnant Myometrium: Evidence for an Autocrine Signaling System at Term*

Complex Interactions Between Sex Steroids and Cytokines in the Human Pregnant Myometrium: Evidence for an Autocrine Signaling System at Term*

Immunologie de la grossesse

Cytokines and the Initiation of Parturition

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