Modulation of the intracellular survival of Brucella abortus by tuftsin and muramyl dipeptide

Price, Robert; Templeton, Joe W.; Smith, R.; Adams, L. Garry

doi:10.1016/0165-2427(93)90024-x

Cited by 7 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To each well, 100 ,lI of 0.01% sodium dodecyl sulfate solution was added in serum-free medium at 37°C for 20 to 30 min. Schneider's medium (GIBCO, Ltd, Paisley, Scotland) supplemented with 30% fetal calf serum and L-glutamine was added (100 RI per well), and the cultures were incubated at 28°C for another 72 h. The cultures in three to six replicates were then pulsed with 1 p.Ci of [3H]thymidine (26 Ci/nmol; Radiochemical Center, Amersham, United Kingdom) per well, and the incorporation of radioactivity by viable parasites after 18 h of further culturing was determined as described above.…”

mentioning

confidence: 99%

The macrophage-activating tetrapeptide tuftsin induces nitric oxide synthesis and stimulates murine macrophages to kill Leishmania parasites in vitro

et al. 1994

View full text Add to dashboard Cite

The macrophage-activating tetrapeptide tuftsin was able to activate, in a dose-dependent manner, murine macrophages to express nitric oxide (NO) synthase and to produce NO. Tuftsin required lipopolysaccharides for the optimal induction of NO production and synergized with gamma interferon in the induction of NO synthesis. Tuftsin-dependent NO production was sensitive to inhibition by dexamethasone and the NO synthase specific inhibitor LGN-monomethylarginine (L-NMMA). Murine peritoneal macrophages activated by tuftsin were able to kill the amastigotes of the intracellular protozoan parasite Leishmania major in vitro.

show abstract

mentioning

confidence: 99%

The macrophage-activating tetrapeptide tuftsin induces nitric oxide synthesis and stimulates murine macrophages to kill Leishmania parasites in vitro

et al. 1994

View full text Add to dashboard Cite

show abstract

“…A synergistic effect of MDP on many therapeutic agents is well known and its synergy with tuftsin was also checked. Preliminary information on the activity of a mixture of MDP and tuftsin was published in 1993 [10]. Tests performed on macrophage bovine teat revealed that the application of a mixture of tuftsin and MDP intensified the macrophage inhibition effect on intracellular replication of Brucella abortus type 2308.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and biological activity of tuftsin, its analogue and conjugates containing muramyl dipeptides or nor-muramyl dipeptides

Dzierzbicka¹,

Trzonkowski²,

Sewerynek³

et al. 2005

J. Peptide Sci.

View full text Add to dashboard Cite

Several conjugates of muramyl dipeptide (MDP) or nor-muramyl dipeptide (nor-MDP) with tuftsin were synthesized. Conjugates 8a-f were prepared by acylation of protected tuftsin with the isoglutamine carboxyl group of MDP or nor-MDP 2a-f. Also tuftsin analogue 6 (H-Thr-Lys-Pro-Arg(NO2)-OH) was obtained. All synthesized compounds were investigated at the Medical University of Gdansk. The biological activity of the examined compounds was estimated using in vitro cultures of human monocytes and lymphocytes. The substances displayed cytotoxic effects, as was revealed in the viability tests performed. The effects were most probably mediated by the induction of an oxidative burst in monocytes and the stimulation of redox enzymes in lymphocytes. In addition, the analogues turned out to be efficient stimulators of TNFalpha and IL6 secretion by monocytes and lymphocytes. Nevertheless, the secretion of cytokines did not affect the viability of the leukocyte population used in the experiments.The beneficial properties of the compounds examined (mainly 6, 3, 8a and 8c), which implies their usefulness as potential therapeutic agents, are connected with their rapid start of action and more efficient effects compared with tuftsin alone. An in vivo assay on animal models will be performed.

show abstract

Interplay of Food‐Derived Bioactive Peptides with Gut Microbiota: Implications for Health and Disease Management

Hu,

Du,

et al. 2024

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Bioactive peptides (BPs) are protein fragments with beneficial effects on metabolism, physiology, and diseases. This review focuses on proteolytic BPs, which are produced by the action of gut microbiota on proteins in food and have demonstrated to influence the composition of gut microbes. And gut microbiota are candidate targets of BPs to alleviate oxidative stress, enhance immunity, and control diseases, including diabetes, hypertension, obesity, cancer, and immune and neurodegenerative diseases. Despite promising results, further research is needed to understand the mechanisms underlying the interactions between BPs and gut microbes, and to identify and screen more BPs for industrial applications. Overall, BPs offer potential as therapeutic agents for various diseases through their interactions with gut microbes, highlighting the importance of continued research in this area.

show abstract

Modulation of the intracellular survival of Brucella abortus by tuftsin and muramyl dipeptide

Cited by 7 publications

References 29 publications

The macrophage-activating tetrapeptide tuftsin induces nitric oxide synthesis and stimulates murine macrophages to kill Leishmania parasites in vitro

The macrophage-activating tetrapeptide tuftsin induces nitric oxide synthesis and stimulates murine macrophages to kill Leishmania parasites in vitro

Synthesis and biological activity of tuftsin, its analogue and conjugates containing muramyl dipeptides or nor-muramyl dipeptides

Interplay of Food‐Derived Bioactive Peptides with Gut Microbiota: Implications for Health and Disease Management

Contact Info

Product

Resources

About