Modulation of the Expression of Hla Class Ii Antigens by Gamma Interferon and Phorbol Ester Tpa on Myeloid Leukaemic Cell Lines

Alonso, M. C.; Navarrete, C.; Solana, Rafael; Ramı́rez, Rafael; Peña, José; Festenstein, H.

doi:10.1111/j.1744-313x.1986.tb01109.x

Cited by 12 publications

(5 citation statements)

References 11 publications

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“…A further observation from our data is that HLA‐DQ behaves in a manner distinct from HLA‐DR following trauma. This lends further support to the hypothesis of different control mechanisms for each antigen [14,15,18,19].…”

Section: Discussionsupporting

confidence: 79%

“…However, surface receptor density was not studied. There appears to have been an assumption that all class II antigens behave in a similar fashion following trauma, despite evidence of independent control of expression for each class II antigen [14,15]. Unfortunately, a possible relationship between immunological response, surface expression, and genotype has yet to be addressed.…”

Section: Introductionmentioning

confidence: 99%

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Early decrease in surface expression of HLA-DQ predicts the development of infection in trauma patients

Taylor

Gordon

Polk

2000

Clinical and Experimental Immunology

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SUMMARYThe behaviour of human leucocyte antigen-DR (HLA-DR) following injury has been extensively studied. However, the behaviour of other class II antigens following trauma has not been characterized as well, despite evidence that HLA-DQ genotype influences the response to several bacterial antigens. Our study attempts to characterize and analyse the behaviour of HLA-DQ after trauma in patients with and without infection. Twenty-five patients were studied following major injury. Fifteen of the 25 patients developed infection (men 11, women 4); 10 patients developed no infection (men 9, women 1). The mean age was 34^12 years for patients with no infection and 52^20 years for those with infection. Monocyte HLA-DQ surface expression was determined using FITC-labelled antibodies and flow cytometry. Expression was compared with a control population of 11 healthy volunteers. The percentage of monocytes expressing HLA-DQ following trauma was reduced in patients with infection and in those without infection, but returned to normal (days 8±14) only in those patients who did not develop infection. Monocyte HLA-DQ mean channel fluorescence was reduced on day 1, but quickly returned to normal in those patients who subsequently developed infection. Stimulated with lipopolysaccharide, the initial samples of 13 patients who developed infection showed that surface expression on these monocytes could be elevated into the normal range. We conclude that HLA-DQ is an additional early marker of outcome that may not function merely as an immune suppressor. The maintained ability of HLA-DQ to present self-antigens may be important in the initial stages of the host response to injury.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Early decrease in surface expression of HLA-DQ predicts the development of infection in trauma patients

Taylor

Gordon

Polk

2000

Clinical and Experimental Immunology

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“…In addition the induction of the different class I1 locus products by IFN-y in different cell types is not coordinate [28]. DR is induced in the absence of DQ on certain fibroblast [28] and myeloid leukemia lines but is induced with DQ on other leukemic lines [29] and particular melanoma cells [30]. The upregulation of class I1 by IFN-y seems to be controlled by the control boxes situated 5' of each class I1 gene (reviewed [31,321).…”

Section: Discussionmentioning

confidence: 99%

Patterns of major histocompatibility complex class II expression by T cell subsets in different immunological compartments. 2. Altered expression and cell function following activation in vivo

et al. 1993

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This study characterizes antigen-induced phenotypic and functional aspects of major histocompatibility complex (MHC) class II expression on recirculating T cells in efferent lymph. In vivo secondary, but not primary challenge is associated with both kinetic and phenotypic alterations in class II expression by T cells. All three major T cell subsets, CD4+, CD8+ and T19+ (gamma delta T cell receptor), show an approximate four fold increase in the level of MHC class II expression during secondary responses. No changes in B cell expression of class II were seen. Resting efferent lymph T cells are predominantly either class II- or DR+DQ- but this changes to DR+DQ+ after antigenic challenge. The antigen-presenting function of these class II+ T cells was investigated at daily intervals after in vivo antigenic challenge. T cells from non-activated lymph nodes could not induce proliferation of antigen-specific T cells with soluble antigen but were weakly stimulatory in allo-mixed lymphocyte reaction (MLR) at high (> 2:1) stimulator cell ratios. Activated T cells isolated during secondary in vivo responses, and expressing increased quantities of MHC class II, were positive stimulator cells in the MLR. In contrast these cells could not present soluble antigen or trypsin-digested antigen to the T cell lines. In the MLR assays, the relative stimulation by class II+ T cells correlates with the levels of class II expression. We conclude from these experiments that both quantitative and qualitative changes in MHC class II, induced on T cells under physiological conditions, play a role in the regulation of the immune response in vivo but that that role is not simply one of presentation of soluble antigen.

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“…It is well known that IFN-y induces an increased expression of MHC class I antigens on a numbr of cells [26,27]; thus it has been assumed that this increased expression may play a role on NK resistance. However, we cannot confirm this hypothesis as IFN-y failed to induce changes in the levels of HLA antigen expression on the cell lines studied, although a significant reduction of NK susceptibility was observed in all cases.…”

Section: Discussionmentioning

confidence: 99%

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

et al. 1990

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The susceptibility to natural killer (NK) cell-mediated cytotoxicity of 20 cell lines obtained from human solid tumors and their class I histocompatibility antigen (HLA) levels were studied in an attempt to determine whether major histocompatibility complex (MHC) products expressed on cells derived from human solid tumors influence NK susceptibility. The effect of interferon-gamma (IFN-gamma) treatment on these elements was also analyzed. The MHC class I (HLA-ABC, HLA-A and HLA-B) antigen levels and degree of NK lysis were very heterogeneous and no correlation was found on comparison. After treatment with IFN-gamma a marked decrease in NK susceptibility was observed in all the cell lines, including the control line K-562. However, the level of HLA class I expression was not modified in any of the lines with the exception of the K-562, which increased. In some cell lines the expression of HLA class I-like antigens. CDla, b and c, was also measured before and after IFN treatment; however, no correlation was found between CD1 levels and NK susceptibility. Consequently, from our results it is possible to conclude that HLA class I antigens do not play a decisive role in NK susceptibility of cell lines derived from human solid tumors and to suggest that molecules which are not HLA class I antigens but IFN-gamma inducible may confer NK resistance to these lines.

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Modulation of the Expression of Hla Class Ii Antigens by Gamma Interferon and Phorbol Ester Tpa on Myeloid Leukaemic Cell Lines

Cited by 12 publications

References 11 publications

Early decrease in surface expression of HLA-DQ predicts the development of infection in trauma patients

Early decrease in surface expression of HLA-DQ predicts the development of infection in trauma patients

Patterns of major histocompatibility complex class II expression by T cell subsets in different immunological compartments. 2. Altered expression and cell function following activation in vivo

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

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