Modulation of the competition between renaturation and aggregation of lysozyme by additive mixtures

Current understanding and insights towards protein stabilization and activation in deep eutectic solvents as sustainable solvent media

“…2,23,81 Recent publications demonstrate that DESs not only serve as green media for improving protein activity but could also be efficiently used as biocompatible solvents to enhance protein functionalization by prompting structural stability and long term preservation. 8,29,43,82,83 Researchers have published a lot of studies based on the synthesis, toxicity, properties and applications of DESs in diverse fields. However, DESs are poorly understood at the molecular level, particularly for bio-based applications.…”

“…The results manifested that the DES-based aqueous biphasic system was an effective medium for biomolecular separation processes. In the same regard, Sajedi and co-workers 83 studied the effect of different kinds of DES as solution additives on the refolding and aggregation of Lys. Most of the additive mixtures were able to inhibit the aggregation of the enzyme.…”

“…The mixtures containing ChCl-ethylene glycol-Imz, ChCl-glycerol-Imz and Imz-betaineethylene glycol demonstrated much lower protective ability as compared to other additives against aggregation. 83 Additionally, Kashyap and co-workers 40 exploited the structural stability and conformational changes in Lys by incorporating ChCl-urea DES and DES-water mixtures using atomistic molecular dynamics simulations (AMDS). The enzyme structure was observed to be in a partially folded state under both neat and aqueous conditions.…”

“…2,23,81 Recent publications demonstrate that DESs not only serve as green media for improving protein activity but could also be efficiently used as biocompatible solvents to enhance protein functionalization by prompting structural stability and long term preservation. 8,29,43,82,83…”

“…The mixtures containing ChCl–ethylene glycol–Imz, ChCl–glycerol–Imz and Imz–betaine–ethylene glycol demonstrated much lower protective ability as compared to other additives against aggregation. 83…”