Modulation of the acute phase response following a lipopolysaccharide challenge in pigs supplemented with an all-natural Saccharomyces cerevisiae fermentation product

Sanchez, N. C. Burdick; Carroll, J.A.; Broadway, P. R.; Bass, Benjamin; Frank, J.

doi:10.1016/j.livsci.2017.11.022

Cited by 11 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peripheral blood cells from SCFP-supplemented dogs produce reduced quantities of TNFα and IL-6 in response to TLR stimulation ( Lin et al, 2019 ). In accordance with our own data, in an LPS challenge model, SCFP-supplemented piglets were shown to produce enhanced quantities of serum IL-6 and TNFα compared with controls ( Burdick Sanchez et al, 2018 ). In humans, SCFP are inclined to have an overall anti-inflammatory effect, reducing the symptoms of allergic rhinitis ( Moyad et al, 2009 ), cold/flu infection ( Moyad et al, 2008 , 2010 ), and histamine-induced skin inflammation ( Jensen et al, 2015 ); however, acute SCFP treatment results in the increased activation of circulating immune cells and increased serum IFNγ concentrations ( Jensen et al, 2011 ).…”

Section: Discussionsupporting

confidence: 87%

Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves

Mahmoud

Slate

Hong

et al. 2020

Journal of Animal Science

View full text Add to dashboard Cite

The objectives of this study were to determine the effects of oral supplementation with Saccharomyces cerevisiae fermentation products (SCFP; SmartCare and NutriTek; Diamond V, Cedar Rapids, IA) on immune function and bovine respiratory syncytial virus (BRSV) infection in preweaned dairy calves. Twenty-four Holstein x Angus, 1-2 day old calves (38.46 ± 0.91 kg initial body weight) were assigned two treatment groups: control; or SCFP treated, milk replacer with 1 g/d SCFP (SmartCare) and calf starter top-dressed with 5 g/d SCFP (NutriTek). The study consisted of one 31 d period. On d 19-21 of the supplementation period, calves were challenged via aerosol inoculation with BRSV strain 375. Calves were monitored twice daily for clinical signs, including rectal temperature, cough, nasal and ocular discharge, respiration effort and lung auscultation. Calves were euthanized on d 10 post infection (d 29-31 of the supplementation period) to evaluate gross lung pathology and pathogen load. Supplementation with SCFP did not affect body weight (P = 0.762) or average daily gain (P = 0.750), percentages of circulating white blood cells (P < 0.05), phagocytic (P = 0.427 for neutrophils and P = 0.460 for monocytes) or respiratory burst (P = 0.119 for neutrophils and P = 0.414 for monocytes) activity by circulating leukocytes either before or following BRSV infection, or serum cortisol concentrations (P = 0.321) after BRSV infection. Calves receiving SCFP had reduced clinical disease scores compared to control calves (P = 0.030); reduced airway neutrophil recruitment (P < 0.002); reduced lung pathology (P = 0.031); and a reduced incidence of secondary bacterial infection. Calves receiving SCFP shed reduced virus compared to control calves (P = 0.049) and tended towards lower viral loads in the lungs (P = 0.051). Immune cells from the peripheral blood of SCFP treated calves produced increased (P < 0.05) quantities of IL-6 and TNFα in response to toll-like receptor stimulation; while cells from the bronchoalveolar lavage (BAL) of SCFP treated calves secreted less (P < 0.05) proinflammatory cytokines in response to the same stimuli. Treatment with SCFP had no effect on virus-specific T cell responses in the blood, but resulted in reduced (P = 0.045) virus-specific IL-17 secretion by T cells in the BAL. Supplementing with SCFP modulates both systemic and mucosal immune responses and may improve the outcome of an acute respiratory viral infection in preweaned dairy calves.

show abstract

Section: Discussionsupporting

confidence: 87%

Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves

Mahmoud

Slate

Hong

et al. 2020

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…The post-LPS decline in circulating platelets, white blood cells, and differentials was expected and is a normal response to LPS challenge ( Jacobsen et al, 2005 ; Burdick Sanchez et al, 2018 ). This decrease in circulating immune cells occurs as the immune cells leave circulation and extravasate into tissues in search of the infection ( Middleton et al, 2002 ).…”

Section: Discussionmentioning

confidence: 96%

“…There are varying responses in pigs and cattle supplemented with other SCFP. A study utilizing a similar SCFP in weaned pigs reported greater baseline intraperitoneal temperature in supplemented pigs compared to control pigs prior to an LPS challenge; however, the change in intraperitoneal temperature following the challenge was not affected by supplementation with the similar SCFP ( Burdick Sanchez et al, 2018 ). A study in pigs challenged with Salmonella found no effect of SCFP supplementation when rectal temperature was measured weekly ( Price et al, 2010 ), while dairy calves supplemented with an SCFP and challenged with Salmonella had decreased rectal temperature compared to control calves when rectal temperature was recorded daily ( Brewer et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Some aspects of the acute phase immune response to a lipopolysaccharide (LPS) challenge are mitigated by supplementation with a Saccharomyces cerevisiae fermentation product in weaned beef calves1,2

Sanchez

Carroll

Broadway

et al. 2020

Translational Animal Science

View full text Add to dashboard Cite

This study was conducted to determine if feeding a Saccharomyces cerevisiae fermentation product (SCFP) to calves would alter the acute phase response to a lipopolysaccharide (LPS) challenge. Crossbred steer calves (n = 32; 274 ± 1.9 kg BW) were randomly allotted to 2 treatment diets for 21 d: 1) Control, fed RAMP (Cargill, Dalhart, TX), and 2) SCFP, fed the Control ration supplemented with NaturSafe at 12 g·hd-1·d-1 mixed into the TMR (NaturSafe®, Diamond V, Cedar Rapids, IA). On d 22, steers were fitted with indwelling jugular catheters and rectal temperature monitoring devices and placed in individual bleeding stalls. On d 23 steers were challenged i.v. with 0.25 µg/kg body weight LPS. Blood samples were collected at 0.5-h (serum) or 2-h (complete blood counts) intervals from -2 to 8 h and again at 24 h relative to the LPS challenge at 0 h. Sickness behavior scores (SBS) were recorded after collection of each blood sample. Rectal temperatures were greater in SCFP steers from 6 to 11 h, at 13 h, from 15 to 20 h, and from 22 to 24 h following the LPS challenge compared to Control steers (treatment × time: P = 0.01). Additionally, SCFP-supplemented steers had reduced (P < 0.01) SBS compared to Control steers. Platelet concentrations remained greater in SCFP-supplemented steers compared to Control steers throughout the study (P = 0.05), while there was a tendency (P = 0.09) for SCFP steers to have greater white blood cells and eosinophils concentrations than Control steers. There was a treatment × time interaction for serum cortisol and glucose (P < 0.01). Specifically, cortisol was greater at 0.5 and 2 h post-challenge but was reduced at 3 h for SCFP steers compared to Control steers. Glucose was greater in SCFP steers at -0.5, 2, and 7.5 h compared to Control steers. Serum amyloid A was reduced in SCFP steers at 0.5 h, yet greater at 1 and 7.5 h post-challenge compared to Control steers (treatment × time: P < 0.01). Fibrinogen concentrations were greater (P < 0.01) in SCFP compared to Control steers. There was a treatment × time interaction (P < 0.01) for tumor necrosis factor-α such that concentrations were reduced in SCFP steers from 1 to 2 h post-challenge compared to Control steers. Overall these data suggest that supplementing calves with SCFP may have primed the innate immune response prior to the challenge, particularly platelets, which resulted in an attenuated sickness behavior and TNF-α response to LPS.

show abstract

“…Pigs were provided corn/soybean meal-based treatment rations that was formulated to meet or exceeded NRC recommendations and water ad libitum for 22 d. Pigs and feeders were weighed weekly, and feed was added to feeders as needed to allow for ad libitum feed intake. On d 11, pigs were anesthetized and a small incision (2 to 2.5 cm) was made in the lower abdominal region for the placement of an indwelling temperature recording device (Star-Oddi DST micro-T; MeterMall USA, Marysville, Ohio, USA) into the peritoneal cavity which measured intraperitoneal temperature continuously every 5-min until the completion of the study (7). Specifically, pigs were placed ventral side up on a v-trough and mask-induced with 5% isoflurane and an oxygen flow rate of 2.0–3.0 L/min.…”

Section: Methodsmentioning

confidence: 99%

“…Feed additives offer many advantages, are easily incorporated into swine management procedures, and there are a large variety of products available to producers that claim to improve animal health. Live yeast and yeast cell wall products have the potential to enhance the acute phase response to various immune challenges (5–7). Recent studies in cattle have found variations in the immune and metabolic responses between live yeast and yeast cell wall, and also between different strains and products (8–11).…”

Section: Introductionmentioning

confidence: 99%

Changes in the Hematological Variables in Pigs Supplemented With Yeast Cell Wall in Response to a Salmonella Challenge in Weaned Pigs

Sanchez

Carroll

Corley³

et al. 2019

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Stressors experienced by pigs at weaning may negatively impact health and productivity. Thus, supplements that enhance pig immunity during the early post-weaned period are of great interest to the swine industry. The objective of this experiment was to evaluate the performance and hematological responses of weaned pigs supplemented with yeast cell wall (YCW) when challenged orally with Salmonella Typhimurium . Weaned pigs were assigned to one of three treatments for 22d ( n = 13/treatment): Control diet, which was a non-medicated starter diet (Control); Control diet supplemented with YCW at 250 mg/kg BW (YCW250; Phileo Lesaffre Animal Care, Milwaukee, Wisconsin, USA); and Control diet supplemented with YCW at 500 mg/kg BW (YCW500). On d19 blood samples were collected from −6 to 72 h relative to oral Salmonella Typhimurium (1 × 10 6 cfu/pig) challenge. Gain:feed was greater ( P = 0.01) in YCW250 treatment compared to both Control and YCW500 pigs. Baseline intraperitoneal temperature was greater ( P < 0.001) in YCW250 pigs than Control or YCW500 pigs. There was a treatment x time interaction for the change in intraperitoneal temperature ( P < 0.01), post-challenge cortisol, white blood cell counts (WBC), neutrophils, and neutrophil:lymphocyte ratio ( P ≤ 0.03). Control pigs had greater ( P < 0.05) cortisol concentrations than both YCW-supplemented groups at 0 h, but Control pigs had reduced ( P < 0.05) cortisol compared to YCW500 pigs at 24 and 30 h post-challenge. Control pigs had greater ( P < 0.05) WBC counts than both YCW-supplemented groups 6 and 12 h post-challenge, and YCW250 pigs had reduced ( P < 0.01) WBC counts than Control and YCW500 pigs 18 h post-challenge. Neutrophil counts were greater ( P < 0.05) in Control pigs than both YCW-supplemented groups at 6 and 12 h post-challenge and were greater ( P = 0.02) than YCW250 pigs at 18 h post-challenge. Lymphocytes were greater ( P < 0.001) in Control and YCW500 pigs pre- and post-challenge compared to YCW250 pigs. Control pigs had the greatest ( P < 0.001) monocyte counts compared to YCW treatments. There was no effect of yeast supplementation on fecal shedding or Salmonella counts in the rectum, colon or cecum ( P ≥ 0.05). While some differences were observed in intraperitoneal temperature and some hematological variables, data suggests there were minimal effects of yeast supplementation on the acute immune response to Salmonella challenge.

show abstract

Modulation of the acute phase response following a lipopolysaccharide challenge in pigs supplemented with an all-natural Saccharomyces cerevisiae fermentation product

Cited by 11 publications

References 12 publications

Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves

Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves

Some aspects of the acute phase immune response to a lipopolysaccharide (LPS) challenge are mitigated by supplementation with a Saccharomyces cerevisiae fermentation product in weaned beef calves1,2

Changes in the Hematological Variables in Pigs Supplemented With Yeast Cell Wall in Response to a Salmonella Challenge in Weaned Pigs

Contact Info

Product

Resources

About