Modulation of steroidogenesis and xenobiotic biotransformation responses in zebrafish (Danio rerio) exposed to water-soluble fraction of crude oil

Arukwe, Augustine; Nordtug, Trond; Kortner, Trond M.; Mortensen, Anne S.; Brakstad, Odd Gunnar

doi:10.1016/j.envres.2008.02.009

Cited by 92 publications

(49 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although CYP1A and other cytochrome P450 enzymes have been shown to be differentially involved in EROD and BROD activity in mammalian species, the significance of BROD induction is not fully understood in aquatic species. Exposure of fish to crude oil aqueous extracts induced both EROD and BROD activity [33], whereas other chemical exposures differentially induced either EROD or BROD activity [34].…”

Section: Spmd Extracts: Erod and Brod Activitymentioning

confidence: 99%

Environmental contaminant effects on juvenile striped bass in the San Francisco Estuary, California, USA

Spearow

Kota

Ostrach

2010

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

The decline of pelagic organisms in the San Francisco Estuary (SFE) (California, USA) is attributed to several factors, including water diversions, invasive species, and exposure to environmental toxicants. The present study evaluated the effects of environmental contaminants on liver vitellogenin, metallothionein, 7-ethoxyresorufin-O-deethylase (EROD), and benzyloxyresorufin O-deethylase (BROD) activity in juvenile striped bass (Morone saxitilis) in the SFE. Analysis of juvenile striped bass liver extracts revealed site-specific elevations of vitellogenin, metallothionein, and EROD biomarkers across the estuary. Although some striped bass in the estuary showed EROD activity similar to unhandled hatchery controls, several sites in the estuary showed significantly higher EROD activity that was in the range of beta-naphthoflavone (BNF)-injected, positive controls. Overall, EROD activity averaged 283% higher in estuary fish than in hatchery controls. Chemical analyses of extracts from semipermeable membrane devices (SPMDs) deployed in the estuary for one month showed elevated polyaromatic hydrocarbon (PAH) levels. Semipermeable membrane devices extract injections-induced metallothionein and BROD in striped bass livers. These data show that environmental exposures are impacting EROD and other biomarkers in the SFE striped bass population. Previous studies in our laboratory have associated poor larval development with maternal transfer of environmental contaminants. Further studies are needed to monitor contaminant exposures by the use of biomarkers and to integrate them into a more effective pelagic species recovery plan in the SFE.

show abstract

Section: Spmd Extracts: Erod and Brod Activitymentioning

confidence: 99%

Environmental contaminant effects on juvenile striped bass in the San Francisco Estuary, California, USA

Spearow

Kota

Ostrach

2010

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…The male fathead minnow, another small-bodied model teleost, also showed down-regulation of Star mRNA when exposed to 10 ng EE2 l -1 . Interestingly, the lack of corresponding alteration of steroidogenic (Star and Cyp11a1 mRNA) and biotransformation (Cyp1a mRNA) responses has been demonstrated in adult zebrafish exposed to water-soluble fraction of crude oil (Arukwe et al 2008). In 20dpfZF, the only consistent steroidogenic signal was that of aromatase (cyp19a1b).…”

Section: Alteration Of Steroidogenic Pathway Genesmentioning

confidence: 99%

“…For instance, many exogenous ligands as substrates for xenobiotic biotransformation, as well as being central to activation of estrogen-responsive genes, should be considered. The induction of cyp1a, a biomarker of xenobiotic biotransformation mediated by AhR, is known to show negative correlation with estrogenic response in teleosts (Arukwe et al 2008, Gräns et al 2010. Toxicity studies using in vivo models show authentic responses, since they are based on the acclimatory physiology of the animal; specific to the immediate exposure scenario.…”

Section: Er-and Ahr-mediated "Cross Talk" In Endocrine Responsesmentioning

confidence: 99%

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

Sahoo

Oikari

2014

J Soils Sediments

View full text Add to dashboard Cite

To assess xenoestrogenicity, the 20 days-post-fertilization zebrafish (20dpfZF) life-stage was experimentally exposed to known model chemicals as well as to environmentally contaminated samples. From a selection of key responsive genes, molecular responses associated with the steroidogenic impact (brain aromatase, cyp19a1b and vitellogenin isoform 1, vtg1) were used to assess the endocrinic modulation by xenoestrogenic samples. This work showed diverse in vivo responses by the 20dpfZF, from pure chemicals (bisphenol A, BPA; 17 -ethinylestradiol, EE2; nonylphenol, NP; octylphenol, OP), and their mixture combinations, and to industrially contaminated sediment samples. Gene expression analysis of cyp19a1b and vtg1 in exposed 20dpfZF showed the estrogenic potencies of chemicals in the order: EE2 > OP > BPA > NP. Transcriptional up-regulation of vtg1 in early juvenile fish exposed to mixtures (BPA + NP + OP), relative to individual concentrations, suggest in minimum the additive in vivo effect of a mixture of very low sublethal doses of single xenoestrogens. Complementary expression of cyp19a1b and vtg1 in 20dpfZF, exposed to artificially-spiked sediment with EE2 established the estrogenresponsiveness of the model. In contrast, the lack of simple estrogenic or antiestrogenic trait of correspondence in biomarker transcription was evident from exposure to two types of industrially contaminated sediments, suggesting common non-linearity of in vivo response by the model. The results also highlight the applicability of 20dpfZF in the assessment of remediation and monitoring of historically polluted sites. This study showed that the 20dpfZF addressed the regulatory requirement of a multi-purpose and cost-effective ecotoxicological model capable of in vivo genomic assessment of endocrineactive substances in aquatic samples.

show abstract

“…The present study showed that the expression levels of steroidogenic genes (together with sex differentiation-related, steroid receptor, and structural genes), were significantly higher at 40 and/or 48 dph than at other time points, with the exception of Pcc-vasa, Pcc-er alpha2, Pcc-esr2a, Pcc-3bhsd, and Pcc-11bhsd2. 3bhsd is associated with the conversion from pregnenolone into progesterone (Arukwe et al, 2008;Evans and Nunez, 2010). Moreover, it is predominantly expressed in the mitochondrion, which is active in the early developmental stages of fish.…”

Section: Time Of Sexual Differentiation In Gynogenic Pccmentioning

confidence: 99%

“…Among the biochemical pathways in the process of steroidogenesis, steroidogenic acute regulator protein (star) takes charge of the regulatory step during acute steroid production for the transfer of cholesterol into pregnenolone across the mitochondrial membrane, which is mediated by the cytochrome P450-mediated side-chain cleavage enzyme (P450scc, cyp11a1). 3b-hydroxysteroid dehydrogenase (3bhsd) is a key enzyme that catalyzes the synthesis of potent steroid hormones (Arukwe et al, 2008). Both hydroxylase and lyase activity of cytochrome 17a-hydroxylase/17, 20-lyase 1 (cyp17a1) are required for the synthesis of testosterone (Sampath Kumar et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Large-scale tissue-specific and temporal gene expression profiles in Pengze crucian carp

Zheng¹,

Chen²,

Wang³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. In the present study, the tissue-specific and temporal gene expression profiles of four catalogues of gonadal development-related genes (sex differentiation-related, steroid receptor, steroidogenic, and structural genes) were detected in nine tissues and during 11 successive developmental stages in the Pengze crucian carp (Pcc) (a triploid monofemale gynogenic fish). The results showed that these target genes exhibited overlapping distributions in various tissues, with the exception of Pcc-vasa and Pcc-cyp17a1. Gene expression profiling of the developmental stages showed that all of the target genes simultaneously reached peak expression levels at 40 and 48 days post hatching (dph). Both 40 and 48 dph appeared to be two key time points associated with the process of Pcc gonadal development. These data will provide a clear understanding of gene expression patterns associated with the gonadal developmentrelated genes of this gynogenic teleost.

show abstract

Modulation of steroidogenesis and xenobiotic biotransformation responses in zebrafish (Danio rerio) exposed to water-soluble fraction of crude oil

Cited by 92 publications

References 65 publications

Environmental contaminant effects on juvenile striped bass in the San Francisco Estuary, California, USA

Environmental contaminant effects on juvenile striped bass in the San Francisco Estuary, California, USA

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

Large-scale tissue-specific and temporal gene expression profiles in Pengze crucian carp

Contact Info

Product

Resources

About