Modulation of scar tissue formation in injured nervous tissue cultivated on surface‐engineered coralline scaffolds

Weiss, Orly Eva; Hendler, Roni Mina; Canji, Eyal; Morad, Tzachy; Foox, Maytal; Francis, Yitshak I.; Dubinski, Zvy; Merfeld, Ido; Hammer, Liat; Baranes, Danny

doi:10.1002/jbm.b.34037

Cited by 9 publications

(14 citation statements)

References 42 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The milling protocol followed in this work was able to improve particle size distribution when compared to previously published data of mollusk shells CaCO3 particles obtaining a narrow particle size distribution especially for OY particles (Hamester, Balzera, & Becker, 2012). bioactivity, osteointegration and osteoinduction (Alakpa et al, 2017;Chroscicka et al, 2016;Decambron et al, 2017;Weiss et al, 2017). In this study we reported the ability of calcium carbonate particles derived from invertebrate shells to promote ECM mineralization and osteoblastic differentiation of MSC when incorporated at 7 mg/ml into alginate hydrogels.…”

Section: Discussionmentioning

confidence: 60%

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

Díaz‐Rodríguez

García‐Triñanes

López

et al. 2018

Carbohydrate Polymers

View full text Add to dashboard Cite

The search for an ideal bone tissue replacement has led to the development of new composite materials designed to simulate the complex inorganic/organic structure of bone. The present work is focused on the development of mineralized calcium alginate hydrogels by the addition of marine derived calcium carbonate biomineral particles. Following a novel approach, we were able to obtain calcium carbonate particles of high purity and complex micro and nanostructure dependent on the source material. Three different types of alginates were selected to develop inorganic/organic scaffolds in order to correlate alginate composition with scaffold properties and cell behavior. The incorporation of calcium carbonates into alginate networks was able to promote extracellular matrix mineralization and osteoblastic differentiation of mesenchymal stem cells when added at 7 mg/ml. We demonstrated that the selection of the alginate type and calcium carbonate origin is crucial to obtain adequate systems for bone tissue engineering as they modulate the mechanical properties and cell differentiation.

show abstract

Section: Discussionmentioning

confidence: 60%

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

Díaz‐Rodríguez

García‐Triñanes

López

et al. 2018

Carbohydrate Polymers

View full text Add to dashboard Cite

show abstract

“…The aragonite crystalline surface of the coral skeleton is a strong adhesive substrate for various isolated cells in culture, including osteocytes, mesenchymal stem cells, neurons and glial cells as well as nervous tissue ex vivo . This coralline biomaterial has a positive influence on these cells, increasing their survival and activity .…”

Section: Discussionmentioning

confidence: 99%

“…We have previously demonstrated that scaffolds derived from the skeleton of the corals Porites lutea and Trachyphyllia geoffroyi are adhesive and permissive for dissociated hippocampal neuronal tissue cultures . The scaffold nurtures the cells with its calcium ions, enhances the survival, growth and connectivity among the cultured neurons and elevates astrocytic hypertrophy . Thus the coral skeleton has considerable potential as an implant for tissue engineering of the nervous system …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Coralline Skeleton Biomaterial Reduces Phagocytosis in Mouse Blood in vitro

Gancz

Zueva

Weiss

et al. 2020

Israel Journal of Chemistry

Self Cite

View full text Add to dashboard Cite

Inflammatory and immunogenic response to foreign bodies presents a challenge in the use of biomaterials as implants for tissue restoration. Therefore, there is a need to understand the interactions between such implants and the blood. One such material, currently in clinical use for bone replacement in humans, is the skeleton of corals, in the form of crystalline aragonite. This biomaterial has been shown to impart a protective and supportive influence on several types of cells ex vivo. The carbonate skeleton activates secretion in phagocytes in vitro, however its effects on these cells in the blood, and on the process of phagocytosis itself, remain unknown. Using 1–500 μm particles of coral skeleton, we show that these particles bind blood proteins and alter the leukocyte population, reducing the proportion of granulocytes by more than 3‐fold with no effect on the proportion of monocytes. In addition, the presence of coral skeleton in the blood causes a reduction in phagocytosis. Specifically, we observed a decrease in the percentage of phagocytic cells by 27 % in the granulocytes and by 73 % in monocyte family, as well as a 41.6 % reduction in the MFI of granulocytes, but with no such effect on monocytes. Taken together, the results suggest that the coral skeleton biomaterial may act as a strong, promotive scaffold for tissue regeneration due to its ability to reduce its rejection by inflammatory reactions such as phagocytosis.

show abstract

“…PDL enhances adherence of various cell types [19] including neurons and glia [20,21]. Indeed, PDL-coated coralline scaffolds (CS-PDL) are superior matrices for the cultivation of neural cells from the hippocampi of postnatal rat brains [8,[22][23][24][25][26]. Moreover, CS-PDL scaffolds are permissive and supportive for cultivated hippocampal tissue [23].…”

Section: Introductionmentioning

confidence: 99%

Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis

Morad¹,

Hendler²,

Canji³

et al. 2021

Prime Archives in Polymer Technology

Self Cite

View full text Add to dashboard Cite

Biomaterials, especially when coated with adhesive polymers, are a key tool for restorative medicine, being biocompatible and supportive for cell adherence, growth, and function. Aragonite skeletons of corals are biomaterials that support survival and growth of a range of cell types, including neurons and glia. www.videleaf.com However, it is not known if this scaffold affects neural cell migration or elongation of neuronal and astrocytic processes, prerequisites for initiating repair of damage in the nervous system. To address this, hippocampal cells were aggregated into neurospheres and cultivated on aragonite skeleton of the coral Trachyphyllia geoffroyi (Coral Skeleton (CS)), on naturally occurring aragonite (Geological Aragonite (GA)), and on glass, all pre-coated with the oligomer poly-D-lysine (PDL). The two aragonite matrices promoted equally strong cell migration (4.8 and 4.3-fold above glass-PDL, respectively) and axonal sprouting (1.96 and 1.95-fold above glass-PDL, respectively). However, CS-PDL had a stronger effect than GA-PDL on the promotion of astrocytic processes elongation (1.7 vs. 1.2-fold above glass-PDL, respectively) and expression of the glial fibrillary acidic protein (3.8 vs. and 1.8-fold above glass-PDL, respectively). These differences are likely to emerge from a reaction of astrocytes to the degree of roughness of the surface of the scaffold, which is higher on CS than on GA. Hence, CS-PDL and GA-PDL are scaffolds of strong capacity to derive neural cell movements and growth required for regeneration, while controlling the extent of astrocytic involvement. As such, implants of PDL-aragonites have significant potential as tools for damage repair and the reduction of scar formation in the brain following trauma or disease.

show abstract

Modulation of scar tissue formation in injured nervous tissue cultivated on surface‐engineered coralline scaffolds

Cited by 9 publications

References 42 publications

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

Coralline Skeleton Biomaterial Reduces Phagocytosis in Mouse Blood in vitro

Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis

Contact Info

Product

Resources

About