Modulation of ROS in nanosecond-pulsed plasma-activated media for dosage-dependent cancer cell inactivation in vitro

Abstract

Dosage control of reactive oxygen and nitrogen species (RONS) is critical to low-temperature plasma applications in cancer therapy. Production of RONS by atmospheric pressure, nonequilibrium plasmas in contact with liquid may be modulated via plasma conditions including plasma treatment time and pulse voltage and repetition frequency. In this study, a terephthalic acid-based probe was used to measure hydroxyl radicals [OH(aq)] in water exposed to plasma and to demonstrate that the OH(aq) concentration increases linearly with treatment time. Fluorometric measurements of hydrogen peroxide concentration in plasma-activated water show a linear relationship between the H2O2 production rate and the pulse repetition frequency of the plasma. In vitro plasma treatment of cancer cells shows that pancreatic (Pan02) and breast (4T1-Luc) cancer cells have different sensitivities to plasma exposure. The dependence of Pan02 cell viability on plasma treatment time or pulse voltage is nonlinear. The system described here for generation and delivery of reactive oxygen species from a nanosecond pulsed plasma jet represents a promising alternative approach to cancer therapy.

Characterization of an atmospheric pressure plasma jet producing the auroral transition O(1S) to O(1D)

“…Due to its interdisciplinary nature, research on APPJs is continuously growing and as a result is developing into an enabling technology for a broad range of applications that support modern society. These plasmas have been widely utilized in applications such as: material processing [5], plasma catalysis [6], wastewater treatment [7,8], cancer therapy [9], sterilization [10], agriculture [11] and food processing [12].…”

Synergistic effects of nanosecond pulsed plasma and electric field on inactivation of pancreatic cancer cells in vitro

“…The parameters used for the treatment protocols of the ns-APPJ generation were chosen to ensure steady and repeatable plasma generation as well as to maintain consistency with our previous work 21 . Also, as studied by 50 , the parameters do not fall in the range of breakdown that could lead to heating-related damage to the cells.…”

“…The degree of their effects on cells is influenced by various parameters such as the plasma working frequency, the voltage waveform, exposure time, and distance from the treated target 17 – 20 . Among other cold plasmas, nanosecond pulsed plasma jets (ns-APPJ) have the potential to offer great flexibility in treatment dosage with a controlled RONS production modulation 21 .…”

Estimation of total forces of jets on liquid interfaces using image processing methods

“…Typical devices include plasma in a liquid and atmospheric-pressure plasma jets in contact with liquid. They are widely used in advanced oxidation processing [5][6][7][8][9], nanoparticle synthesis [10][11][12][13], plasma medicine and water treatment [14][15][16][17][18][19][20][21]. To ascertain physical and chemical processes occurring in plasma-liquid reactors, Liu et al investigated the plasma jet dynamics above the gas-liquid interface * Author to whom any correspondence should be addressed.…”