Modulation of reproductive exhaustion usingUlva clathratain Pacific white shrimpLitopenaeus vannamei(Boone, 1931) broodstock during commercial maturation

Abstract

Ulva clathrata meal (2%) was supplemented in a fresh maturation diet for Litopenaeus vannamei broodstock in a commercial larval facility and its effects on reproductive performance, histology and histochemistry of gonad, and biochemical composition of gonad, hepatopancreas, and muscle were analysed. Females that were given Ulva in the diet had reduced mortality compared to controls, with no differences in males. Females fed Ulva also had bigger oocytes but only those in late vitellogenesis (+7%, p < 0.01) and postvitellogenesis (+15%, p < 0.01); they also had a higher proportion of late vitellogenic oocytes in maturation stage II, and a bigger area occupied by cortical rods. No significant differences were found for the proportion of previtellogenic or early vitellogenic oocytes per maturation stage or as a result of Ulva. Females fed Ulva had higher levels of lipids in ovary (+35%, p < 0.05), with no significant differences in biochemical reserves in hepatopancreas or muscle. The total number of eggs (+5%, p < 0.01), nauplii per spawn (+18%, p < 0.01), and hatching rate (+13%, p < 0.01) during the productive cycle were higher for Ulva fed shrimp. Individual follow‐up of marked females during the productive cycle showed a decreased reproductive exhaustion in females fed Ulva over consecutive spawns. Apparently, U. clathrata stimulates advanced vitellogenesis by improving lipid accumulation in gonads, and this in turn increases reproductive performance: inclusion of this algae in shrimp diet could be used to decrease reproductive exhaustion during production.

Oogenesis of Lithodes santolla: histological and histochemical characterization

“…In the anomura crab C. clibanarius, lipids are present from early vitellogenesis, as in L. santolla, and suggest an exogenous supplement (Varadarajan & Subramoniam, 1980). The increase of triglycerides and decrease of phospholipids during vitellogenesis and postvitelogénesis has been observed in Pacific white shrimp Litopenaeus vannamei (Corral-Rosales et al, 2018), by the same Sudan Black B stain, used in this study.…”

Interactive influence of dietary protein and lipid on maturation of Indian white prawn, Penaeus indicus broodstock

“…The complete replacement of fresh feed with formulated diet for penaeid broodstock maturation remains a major challenge for researchers and hatchery operators. In penaeid shrimp, numerous studies were reported regarding the fortification of broodstock diets with bioactive lipids, nutrients and animal extracts in an attempt to duplicate the gonad maturation promoting effects using fresh diets (Chung et al, 2011; Corral‐Rosales, Cruz‐Suárez, et al, 2018; Corral‐Rosales, Palacios, et al, 2018; Huang et al, 2008; Nguyen et al, 2012; Paibulkichakul et al, 2008; Wouters et al, 2002). However, most of the earlier works were met with limited success.…”

“…Protein content of the two best maturation diets used in this study (i.e., 45% and 55%) was within the range of protein levels of combined fresh diets routinely used in hatcheries as well as artificial diets previously reported to be effective for P . indicus (Cahu et al, 1995) Penaeus stylirostris (Bray et al, 1990), Penaeus monodon (Marsden et al, 1997; Paibulkichakul et al, 2008) and Litopenaeus vannamei (Adloo et al, 2020; Corral‐Rosales, Cruz‐Suárez, et al, 2018; Corral‐Rosales, Palacios, et al, 2018; Naessens et al, 1997; Wouters et al, 2002). However, this range of protein inclusion is higher than the protein requirement reported for juvenile P .…”

“…Earlier studies have demonstrated that dietary manipulations can improve the overall reproductive performance of cultured crustaceans. Fresh food containing adequate amount of highly unsaturated fatty acids (HUFA) (Hoa et al, 2009) and diets supplemented with HUFA and astaxanthin (Huang et al, 2008), sodium alginate (Chung et al, 2011), seaweeds (Corral‐Rosales et al, 2018; Corral‐Rosales et al, 2018) and artemia (Nassens et al, 1997; Wouters et al, 2002) were documented to improve the reproductive performance of economically important penaeids. However, most of these studies included fresh diets such as squid, bivalves and polychaetes as major components of the maturation diet.…”

Reproduction in the spider crab Maiopsis panamensis (Decapoda: Majoidea) in the Gulf of California

“…. santolla and byCorral-Rosales et al (2018) in Pacific white shrimp, L. vannamei. These authors used SBB staining for triglycerides and phospholipids and used digital image analysis to quantify blue and gray pixel shades, respectively, as described by Rodríguez-Jaramillo et al(2008).…”