Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach

Morabito, Caterina; Rovetta, Francesca; Bizzarri, Mariano; Mazzoleni, Giovanna; Fanò, Giorgio

doi:10.1016/j.freeradbiomed.2009.12.005

Cited by 94 publications

(76 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It should be mentioned that "in vivo" experiments in literature also seem to confirm that chronic exposure to ELF-EMF is able to generate a stress oxidative status [32][33][34]. The "in vitro" experiments reported here, as well as the results derived from "in vivo" techniques [13,22,24,25,28,35,36] in mice, show that the presence of ELF-EMF can induce a variable and specie-specific alteration of the stress oxidative pathway.…”

Section: Discussionsupporting

confidence: 79%

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Farina

Mariggiò³

et al. 2010

PIER B

View full text Add to dashboard Cite

Abstract-The aim of this paper is to discuss the effects of the exposure to Extremely Low Frequency ElectroMagnetic Fields (ELFEMFs) on non-and excitable cells using in vitro cell models, namely neuron-like cell line (PC12), glioblastoma-derived GL15 cells as glial model and C2C12 myocytes as muscle model, focusing our attention on standardized protocols for ELF-EMFs generation and exposure. A major issue in laboratory -and likely in nature -studies about possible biological effects of ELF waves is the difficulty in providing standard, reproducible environmental conditions. Hence, as part of the work we have developed an exposure system including a probing scanner, able to sample a given volume and to measure the timevarying magnetic field vector. The system allows detection, monitoring and removal of electromagnetic noise sources, as well as means to assess field homogeneity in terms of intensity and polarization.

show abstract

Section: Discussionsupporting

confidence: 79%

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Farina

Mariggiò³

et al. 2010

PIER B

View full text Add to dashboard Cite

show abstract

“…a remarkable amount of data support another interesting theory that find in chemical reactions involving free radical production, the target of ELF-EMFs [29]. In fact in the last years, some data from literature have described redox-related cellular changes, following exposure to ELF-EMFs [10,27,[30][31][32].…”

Section: Introductionmentioning

confidence: 83%

“…Total protein concentrations were quantified using a colorimetric assay (Bio-Rad Laboratories). Catalase activity was determined based on the decrease in absorbance due to H 2 O 2 consumption (ε= -0.04 mM -1 cm -1 ) measured at 240 nm, according to the method described by Morabito and colleagues [27]. The final reaction volume of 1 ml contained 100 mM Na-phosphate buffer (pH 7.0), 12 mM H 2 O 2 , and 100 μg of sample.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

Effects of Acute and Chronic Low Frequency Electromagnetic Field Exposure on PC12 Cells during Neuronal Differentiation

Morabito

Guarnieri

Fanò³

et al. 2010

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims. The purpose of this study was to provide information about the in vitro neuritogenesis during cell exposure to extremely low frequency electromagnetic fields (ELF-EMFs) of different intensities and durations using pheochromocytoma-derived cell line (PC12 cells) as neuronal model. Methods. Proliferative rates and neuritogenesis were tested by colorimetric assay and morphological analysis, respectively; reactive oxygen species (ROS) levels and intracellular Ca²⁺ variations monitored using single cell videomicroscopy. Results. The long-lasting ELF-EMF exposure (0.1-1.0 mT) did not appear to significantly affect the biological response (proliferation and neuritogenesis). However, during the acute ELF-EMF exposure (30 min), in undifferentiated PC12 cells, there were increased ROS levels and decreased catalase activity, that, conversely, resulted increased after chronic exposure (7 days) at 1.0 mT. Acute exposure (0.1-1.0 mT) affected the spontaneous intracellular Ca²⁺ variations in undifferentiated cells, in which basal intracellular Ca²⁺ resulted increased after chronic exposure. In addition acute exposure affected cell response to a depolarizing agent, while basal membrane potential was not changed. Conclusion. Even if further studies remain necessary to identify the ROS/intracellular Ca²⁺cross-talking pathway activated by ELF-EMF exposure, we support the hypothesis that ROS and Ca²⁺ could be the cellular “primum movens” of the ELF-EMF induced effects on biological systems.

show abstract

“…Calcium handling affected by EMFs was studied in undifferentiated C2C12 myoblasts, as well as in terminally differentiated myotubes [159]. The authors revealed that exposure to EMFs increased the percentage of cells with voltage-dependent calcium influx in immature myoblasts, while in mature myotubes, the cytosolic calcium concentration was elevated due to an enhanced activity of the intracellular calcium stores.…”

Section: Mechano-gated Ion Channel Modulation: Converting Mechanical mentioning

confidence: 99%

Calcium's Role in Mechanotransduction during Muscle Development

Damm

Egli

2014

Cell Physiol Biochem

11,388

View full text Add to dashboard Cite

Mechanotransduction is a process where cells sense their surroundings and convert the physical forces in their environment into an appropriate response. Calcium plays a crucial role in the translation of such forces to biochemical signals that control various biological processes fundamental in muscle development. The mechanical stimulation of muscle cells may for example result from stretch, electric and magnetic stimulation, shear stress, and altered gravity exposure. The response, mainly involving changes in intracellular calcium concentration then leads to a cascade of events by the activation of downstream signaling pathways. The key calcium-dependent pathways described here include the nuclear factor of activated T cells (NFAT) and mitogen-activated protein kinase (MAPK) activation. The subsequent effects in cellular homeostasis consist of cytoskeletal remodeling, cell cycle progression, growth, differentiation, and apoptosis, all necessary for healthy muscle development, repair, and regeneration. A deregulation from the normal process due to disuse, trauma, or disease can result in a clinical condition such as muscle atrophy, which entails a significant loss of muscle mass. In order to develop therapies against such diseased states, we need to better understand the relevance of calcium signaling and the downstream responses to mechanical forces in skeletal muscle. The purpose of this review is to discuss in detail how diverse mechanical stimuli cause changes in calcium homeostasis by affecting membrane channels and the intracellular stores, which in turn regulate multiple pathways that impart these effects and control the fate of muscle tissue.

show abstract

Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach

Cited by 94 publications

References 42 publications

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Effects of Acute and Chronic Low Frequency Electromagnetic Field Exposure on PC12 Cells during Neuronal Differentiation

Calcium's Role in Mechanotransduction during Muscle Development

Contact Info

Product

Resources

About