Modulation of ras Transformation Affecting Chromatin Supraorganization as Assessed by Image Analysis

Mello, Maria Luiza S.; Contente, Sara; Vidal, Benedicto de Campos; Planding, Wolfgang; Schenck, U

doi:10.1006/excr.1995.1328

Cited by 63 publications

(54 citation statements)

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“…39 Furthermore, this nuclear form of LOX was shown to exhibit intra-nuclear catalytic activity. 5 That LOX could affect nuclear function was confirmed by the observation of a less tight chromatin packing state directly induced by LOX transfection, 40 which may be a mechanism that influences the expression of many genes and thus the growth and differentiated state of colonic epithelial cells. Alternatively, LOX might affect a main cell growth control mechanism.…”

Section: Discussionmentioning

confidence: 96%

Somatic mutations of the lysyl oxidase gene on chromosome 5q23.1 in colorectal tumors

Csiszár

Fong

Újfalusi

et al. 2001

Intl Journal of Cancer

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Section: Discussionmentioning

confidence: 96%

Somatic mutations of the lysyl oxidase gene on chromosome 5q23.1 in colorectal tumors

Csiszár

Fong

Újfalusi

et al. 2001

Intl Journal of Cancer

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“…The results reported here were especially based on the analysis of the distribution of the Feulgen-stained cell nuclei in a scatter diagram that plots relative ''condensed'' chromatin areas (S C %) versus textural contrast between ''condensed'' and noncondensed chromatin (26)(27)(28)(29). This representation is useful, since it is not affected by the degree of nuclear ploidy, which varies, in the Malpighian tubules of M. quadrifasciata and M. rufiventris (3).…”

Section: Discussionmentioning

confidence: 99%

“…These diagrams allow the discrimination of the position of points corresponding to specific nuclear phenotypes (26)(27)(28)(29).…”

Section: S C Area In Lmmentioning

confidence: 99%

DNA methylation in stingless bees with low and high heterochromatin contents as assessed by restriction enzyme digestion and image analysis

Mampumbu

Mello

2006

Cytometry Pt A

Self Cite

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Background: The stingless bee genus Melipona has been divided into two groups, based on their heterochromatin content. Melipona quadrifasciata and Melipona rufiventris have low and high levels of heterochromatin, respectively. Since condensed chromatin may be rich in methylated DNA sequences, M. quadrifasciata and M. rufiventris nuclei may contain different amounts of methylated CpG. These differences could be assessed by comparing Feulgen-DNA values obtained by image analysis of cells treated with the restriction enzymes Msp I and Hpa II that distinguish between methylated and unmethylated DNA. Msp I and Hpa II cleave the sequence -CCGG-, but there is no cleavage by Hpa II if the cytosine of the central CG dinucleotide is methylated. Methods: Malpighian tubules of M. quadrifasciata and M. rufiventris were treated with Msp I and Hpa II prior to

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“…Although the traditional function attributed to the LOX is initiation of the covalent crosslinking of extracellular collagens and elastin, other studies have shown that LOX can be found in the nucleus, where it may regulate gene expression as evidenced by interaction with histones, altered chromatin condensation, inactivation of NF-B, and modulation of ␤-catenin and cyclin D1 expression. [29][30][31][32][33][34][35][36] Recently, nuclear LOX activity, specifically deactivation of NF-B, has been directly linked to the cleaved 18 kDa LOX-PP (rather than the active 34 kDa LOX enzyme), which has been suggested to support a tumor suppressor function. 37 Outside the nucleus, LOX can induce chemotaxis in monocytes, fibroblasts, and smooth muscle cells.…”

Section: Le Et Almentioning

confidence: 99%

Validation of Lysyl Oxidase As a Prognostic Marker for Metastasis and Survival in Head and Neck Squamous Cell Carcinoma: Radiation Therapy Oncology Group Trial 90-03

Harris²,

Magliocco³

et al. 2009

JCO

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Purpose To validate lysyl oxidase (LOX), a hypoxia-related protein, as a marker for metastasis in an independent head and neck cancer (HNC) patient group enrolled onto a prospective trial. Patients and Methods We performed traditional immunohistochemical (IHC) staining and automated quantitative analysis (AQUA) for LOX expression in 66 HNC patients from one institution. We also performed AQUA staining for LOX in 306 of 1,113 patients treated on a phase III trial comparing four radiation fractionation schedules in locally advanced HNC (RTOG 90-03). Pretreatment characteristics and outcome were similar between patients with and without LOX assessment. We correlated AQUA LOX expression with time to metastasis (TTM), time to progression (TTP), and overall survival (OS). Results LOX expression from both staining methods predicted for TTM in the first 66 patients. Multivariate analysis, controlling for significant parameters including nodal stage and performance status, revealed tumor LOX expression, as a continuous variable, was an independent predictor for TTM (hazard ratio [HR], 1.21; 95% CI, 1.10 to 1.33; P = .0001), TTP (HR, 1.06; 95% CI, 1.02 to 1.10; P = .0069), and OS (HR, 1.04; 95% CI, 1.00 to 1.07; P = .0311) in RTOG 90-03 patients. This translates into a 259% increase in metastatic risk for a patient at the 75th percentile of LOX compared with one at the 25th percentile. Conclusion AQUA LOX expression was strongly associated with increased metastasis, progression, and death in RTOG 90-03 patients. This study validates that LOX is a marker for metastasis and survival in HNC.

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Modulation of ras Transformation Affecting Chromatin Supraorganization as Assessed by Image Analysis

Cited by 63 publications

References 0 publications

Somatic mutations of the lysyl oxidase gene on chromosome 5q23.1 in colorectal tumors

Somatic mutations of the lysyl oxidase gene on chromosome 5q23.1 in colorectal tumors

DNA methylation in stingless bees with low and high heterochromatin contents as assessed by restriction enzyme digestion and image analysis

Validation of Lysyl Oxidase As a Prognostic Marker for Metastasis and Survival in Head and Neck Squamous Cell Carcinoma: Radiation Therapy Oncology Group Trial 90-03

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