Modulation of Quorum Sensing in a Gram‐Positive Pathogen by Linear Molecularly Imprinted Polymers with Anti‐infective Properties

Motib, Anfal Shakir; Guerreiro, António; Al-Bayati, Firas A.; Piletska, Elena; Manzoor, Irfan; Shafeeq, Sulman; Kadam, Anagha; Kuipers, Oscar P.; Hiller, Luisa; Cowen, Todd; Piletsky, Sergey A.; Andrew, Peter W.; Yeşilkaya, Hasan

doi:10.1002/ange.201709313

Cited by 12 publications

(4 citation statements)

References 23 publications

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“…The LMIP played an antiinfective role by blocking the quorum sensing (QS) mechanism and preventing virulence of a Gram‐positive pathogen. [ 23 ] QS is a mechanism by which bacteria communicate with each other through the production and identification of signaling molecules. The monomer composition was determined via mass spectrometry and the identified fragments were aligned, along with the peptide template, by computer modeling to predict the possible monomer sequence of the LMIP.…”

Section: Renewable and Innocuous Starting Materials For Molecular Imprintingmentioning

confidence: 99%

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Molecular Imprinting: Green Perspectives and Strategies

et al. 2021

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Large quantities of organic solvents and reagents are usually required in the fabrication of these materials. Moreover, the use of excess reagents in solvent extraction, purification, filtration/ centrifugation, and cleaning processes is essential for adequate product purity. Because of the numerous practical applications of advanced materials, some are synthesized on a large scale and commercialized. [3] The excessive consumption of nonrenewable, hazardous chemicals is an unsustainable practice and a prominent criticism of these materials. Nonetheless, the use of advanced materials in sensing applications, medicine, electronics, and catalysis is inevitable. [4] During various research activities, such as fabricating advanced materials, as well as in the subsequent stage, i.e., industrial operations, new challenges have arisen that impact not only almost every aspect of human life but also other living creatures and ecosystems. It is estimated that the impact of these practices will endure into the next century. [5] Since the advancement of science, industry, and technology is inevitable, scientists have been persuaded to search for methods to counteract or minimize the negative impacts of human activities. [6] Thus, green chemistry has advanced and green principles have been defined for most sciences, including synthesis, [7] analytical chemistry, [8] engineering, [9] and pharmaceutical science. [10] Fortunately, contemporary society has great awareness of deleterious environmental side effects, resulting in a sense Advances in revolutionary technologies pose new challenges for human life; in response to them, global responsibility is pushing modern technologies toward greener pathways. Molecular imprinting technology (MIT) is a multidisciplinary mimic technology simulating the specific binding principle of enzymes to substrates or antigens to antibodies; along with its rapid progress and wide applications, MIT faces the challenge of complying with green sustainable development requirements. With the identification of environmental risks associated with unsustainable MIT, a new aspect of MIT, termed green MIT, has emerged and developed. However, so far, no clear definition has been provided to appraise green MIT. Herein, the implementation process of green chemistry in MIT is demonstrated and a mnemonic device in the form of an acronym, GREENIFICATION, is proposed to present the green MIT principles. The entire greenificated imprinting process is surveyed, including element choice, polymerization implementation, energy input, imprinting strategies, waste treatment, and recovery, as well as the impacts of these processes on operator health and the environment. Moreover, assistance of upgraded instrumentation in deploying greener goals is considered. Finally, future perspectives are presented to provide a more complete picture of the greenificated MIT road map and to pave the way for further development.

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Section: Renewable and Innocuous Starting Materials For Molecular Imprintingmentioning

confidence: 99%

“…Reproduced with permission. [ 23 ] Copyright 2017, Wiley. B) Fabrication of a hydrogel with synergistic effects of cell imprinting and boronate affinity for the capture and release of SMMC‐7721 cells.…”

Section: Renewable and Innocuous Starting Materials For Molecular Imprintingmentioning

confidence: 99%

Molecular Imprinting: Green Perspectives and Strategies

et al. 2021

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“…The concern that the template effect may fail as a result of shape changes is usually in conditions where cross-linker is required, as the specificity in those conditions is dependent on the 3D shape of the template. Researchers have obtained linear soluble polymers that target bacteria and proteins without the addition of cross-linkers, [11,16,25] which means the specificity is not dependent on the 3D shape of the template in this condition. In the current study, the CS-MAP reaction does not need cross-linkers.…”

Section: The Reasons For the Specificity Of Glycosurfacementioning

confidence: 99%

“…Cameron Alexander and Hasan Yesikaya have obtained linear soluble polymers which target bacteria and proteins without the addition of cross-linkers. [11,16] These linear soluble MIT expand application scenarios. However, bio-templates used in linear MIT are commonly microorganisms with relative rigidity like bacteria.…”

Section: Introductionmentioning

confidence: 99%

Surface‐Initiated Synthesis of Cell‐Specific Glycopolymers Using Live Mammalian Cells as Templates

Luo

Zhang

et al. 2023

Macromol. Rapid Commun.

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Molecular recognition is an important process in life activities where specificity is the key. However, the method to gain specificity are often complex and time‐consuming. Herein, a novel, versatile, and effective way is developed to obtain cell‐specific glycosurfaces by surface‐initiated Cu‐mediated reversible deactivation radical polymerization (Cu‐RDRP) in an open to air fashion. Mammalian cells are used for the first time as live templates to realize cell‐sugar monomer‐aptation‐polymerization which can produce cell‐specific glycosurfaces. Both epithelial cell adhesion molecule (EpCAM) positive cells L929 and EpCAM negative cells Hela as models are used to acquire two cell‐specific glycosurfaces, which can distinguish template‐cells from others. The strategy is effective and convenient without the need of fixative pretreatment of cells. It is found that the specific capture does not rely on EpCAM antibodies, and the specificity is related to the composition and chain sequence of the glycopolymer brushes rather than surface morphology. In addition, these glycosurfaces keep the ability to identify the target cells after ten regenerative treatments, which provides another advantage for practical applications.

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Combating Biofilm Associated Infection In Vivo: Integration of Quorum Sensing Inhibition and Photodynamic Treatment based on Multidrug Delivered Hollow Carbon Nitride Sphere

Sun

Qin

Yan

et al. 2019

Adv Funct Materials

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Recently, quorum sensing (QS) inhibitors (QSIs) have been combined with antibiotics to enhance antibiofilm efficacy in vitro and in vivo. However, targeting QS signals alone is not enough to prevent bacterial infections. Drug resistance and recurrence of biofilms makes it difficult to eradicate. Herein, photodynamic therapy (PDT) is selected to unite QSIs and antibiotics. A synergistically antibiofilm system, which combines QSIs, antibiotics, and PDT based on hollow carbon nitride spheres (HCNSs) is envisaged. First, HCNS provides the multidrug delivering ability, enabling QSIs and antibiotics to be released in sequence. Subsequently, multistage releases sensitize bacteria effectively, potentiating the chemotherapeutic effects of the antibiotics. Finally, the integration of QSIs and PDT not only minimizes the possibility of drug resistance, but also overcomes the problem of limited mass and extension of PDT. Even after 48 h of incubation, the bacterial biofilm is obviously inhibited. And its biofilm disperse efficiency exceeds 48% (compared with QSI‐potentiated chemotherapy group) and 40% (compared with PDT group). Besides, the inhibition of the QS system influences phenotypes related to virulence factor production and surface hydrophobicity, which weaken biofilm invasion and formation. Eventually, this system is applied to disperse bacterial biofilm in vivo. Overall, PDT and QS modulation are devoted to eradicate drug resistance and recurrence of the biofilm.

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Modulation of Quorum Sensing in a Gram‐Positive Pathogen by Linear Molecularly Imprinted Polymers with Anti‐infective Properties

Cited by 12 publications

References 23 publications

Molecular Imprinting: Green Perspectives and Strategies

Molecular Imprinting: Green Perspectives and Strategies

Surface‐Initiated Synthesis of Cell‐Specific Glycopolymers Using Live Mammalian Cells as Templates

Combating Biofilm Associated Infection In Vivo: Integration of Quorum Sensing Inhibition and Photodynamic Treatment based on Multidrug Delivered Hollow Carbon Nitride Sphere

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