Modulation of Proliferation and Differentiation of Human Anterior Cruciate Ligament–Derived Stem Cells by Different Growth Factors

Cheng, Ming; Yang, Hui; Chen, Tain Hsiung; Lee, Oscar K.

doi:10.1089/ten.tea.2009.0172

Cited by 23 publications

(22 citation statements)

References 33 publications

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“…In vitro studies suggest that bFGF and PDGF not only stimulate tendon fibroblast proliferation but promote changes in the expression of matrix genes showing promise for improving tendon healing [16]. PDGF also plays a role in the migration and proliferation of the tenocytes, fibroblasts, and MSCs responsible for tissue homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis

et al. 2013

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Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, their exogenous addition can further stimulate stem cells into the tenogenic lineage, which might eventually depend on stem cells source. In the present study we investigate the tenogenic differentiation potential of human- amniotic fluid stem cells (hAFSCs) and adipose-derived stem cells (hASCs) with several GFs associated to tendon development and healing; namely, EGF, bFGF, PDGF-BB and TGF-β1. Stem cells response to biochemical stimuli was studied by screening of tendon-related genes (collagen type I, III, decorin, tenascin C and scleraxis) and proteins found in tendon extracellular matrix (ECM) (Collagen I, III, and Tenascin C). Despite the fact that GFs did not seem to influence the synthesis of tendon ECM proteins, EGF and bFGF influenced the expression of tendon-related genes in hAFSCs, while EGF and PDGF-BB stimulated the genetic expression in hASCs. Overall results on cellular alignment morphology, immunolocalization and PCR analysis indicated that both stem cell source can be biochemically induced towards tenogenic commitment, validating the potential of hASCs and hAFSCs for tendon regeneration strategies.

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Section: Introductionmentioning

confidence: 99%

Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis

et al. 2013

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“…9,10 Recent work by Cheng and colleagues revealed a vivid response of collagenase released ACL progenitors to several growth factors with respect to cell proliferation and differentiation. 45 In animal models, the repair of tendons and ligaments can be enhanced by various morphogens and growth factors, 38 such as BMP12, 46 TGF-b, 47 growth and differentiation factor (GDF)5, 48 and platelet derived growth factor (PDGF). 49 Gene transfer is an attractive technology for delivering these factors, especially if the vectors are associated with the matrix into which the ACL outgrowth cells migrate.…”

Section: Mobile Mscs Within the Acl 1383mentioning

confidence: 99%

Mesenchymal Stem Cell Characteristics of Human Anterior Cruciate Ligament Outgrowth Cells

Steinert

Kunz²,

Prager³

et al. 2011

Tissue Engineering Part A

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When ruptured, the anterior cruciate ligament (ACL) of the human knee has limited regenerative potential. However, the goal of this report was to show that the cells that migrate out of the human ACL constitute a rich population of progenitor cells and we hypothesize that they display mesenchymal stem cell (MSC) characteristics when compared with adherent cells derived from bone marrow or collagenase digests from ACL. We show that ACL outgrowth cells are adherent, fibroblastic cells with a surface immunophenotype strongly positive for cluster of differentiation (CD)29, CD44, CD49c, CD73, CD90, CD97, CD105, CD146, and CD166, weakly positive for CD106 and CD14, but negative for CD11c, CD31, CD34, CD40, CD45, CD53, CD74, CD133, CD144, and CD163. Staining for STRO-1 was seen by immunohistochemistry but not flow cytometry. Under suitable culture conditions, the ACL outgrowth-derived MSCs differentiated into chondrocytes, osteoblasts, and adipocytes and showed capacity to self-renew in an in vitro assay of ligamentogenesis. MSCs derived from collagenase digests of ACL tissue and human bone marrow were analyzed in parallel and displayed similar, but not identical, properties. In situ staining of the ACL suggests that the MSCs reside both aligned with the collagenous matrix of the ligament and adjacent to small blood vessels. We conclude that the cells that emigrate from damaged ACLs are MSCs and that they have the potential to provide the basis for a superior, biological repair of this ligament.

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“…To evaluate the maturation level of MSCs-osteoblast differentiation of, histochemical and molecular biological methods such as alkaline phosphatase staining, Von Kossa staining, Western blot and reverse transcription polymerase chain reaction (RT-PCR) are commonly used [27], [28], [29]. However, all these traditional methods are time-consuming, cell-destructing, and can only offer semi-quantitative or non-quantitative information except for real-time RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Detection of Osteogenic Differentiation by Differential Mineralized Matrix Production in Mesenchymal Stromal Cells by Raman Spectroscopy

Hung¹,

Kuo

Chen³

et al. 2013

PLoS ONE

Self Cite

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Mesenchymal stromal cells (MSCs) hold great potential in skeletal tissue engineering and regenerative medicine. However, conventional methods that are used in molecular biology to evaluate osteogenic differentiation of MSCs require a relatively large amount of cells. Cell lysis and cell fixation are also required and all these steps are time-consuming. Therefore, it is imperative to develop a facile technique which can provide real-time information with high sensitivity and selectivity to detect the osteogenic maturation of MSCs. In this study, we use Raman spectroscopy as a biosensor to monitor the production of mineralized matrices during osteogenic induction of MSCs. In summary, Raman spectroscopy is an excellent biosensor to detect the extent of maturation level during MSCs-osteoblast differentiation with a non-disruptive, real-time and label free manner. We expect that this study will promote further investigation of stem cell research and clinical applications.

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Modulation of Proliferation and Differentiation of Human Anterior Cruciate Ligament–Derived Stem Cells by Different Growth Factors

Cited by 23 publications

References 33 publications

Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis

Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis

Mesenchymal Stem Cell Characteristics of Human Anterior Cruciate Ligament Outgrowth Cells

Detection of Osteogenic Differentiation by Differential Mineralized Matrix Production in Mesenchymal Stromal Cells by Raman Spectroscopy

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