Modulation of post-thaw sperm functions with oviductal proteins in buffaloes

Kumaresan, A.; Ansari, M.R.; Garg, Ashok

doi:10.1016/j.anireprosci.2005.01.009

Cited by 54 publications

(41 citation statements)

References 24 publications

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“…Resultados semelhantes foram descritos por Kumaresan et al [34] que observaram um aumento da viabilidade de espermatozoides de búfalos incubados com proteínas de oviduto. Yao et al [35] também descreveram um aumento da motilidade de espermatozoides humanos cocultivados com células epiteliais de oviduto ou incubados com seu meio condicionado.…”

Section: Resultsunclassified

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Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

et al. 2015

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Cómo citar este artículo: Valleriote P, Paes de Carvalho C, Dubeibe DF, Mogollón-Waltero ÉM, Burla-Dias, ÂJ. Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados. Spei Domus. 2015;11(22) Resumo. Introdução: nos bovinos, o oviduto é fundamental nos processos de maturação, fertilização e aquisição de competências para o adequado desenvolvimento do embrião até blastocisto. Visto que as células do revestimento interno do oviduto proporcionam substâncias que afetam positivamente os espermatozoides, à medida que atravessam o trato genital da fêmea, os componentes da superfície são modificados ou removidos pelas secreções ali presentes; ditas modificações podem incluir depleção do colesterol na superfície espermática, alteração nos glicosaminoglicanos e mudanças nos íons. Metodologia: Objetivou-se avaliar a adição de meio de cultura de células de oviduto bovino sobre parâmetros espermáticos como motilidade, vigor e integridade de membrana citoplasmática. Os espermatozoides foram incubados com 164 μl de Talp-sp-Uso no grupo controle (T Ct ); 161,5 μl de Talp-sp-Uso, acrescido de 2,5 μl de heparina (20µg/ml) no tratamento 1 (T Hep ) e 128 μl de Talp-sp uso, acrescido de 36 μl de meio de cultivo com secreções de células do oviduto bovino no Tratamento 2 (T Ov ). Foram avaliados no tempo zero (para mostrar que os meios dos tratamentos não prejudicaram os espermatozoides) e após três horas de incubação. Resultados: não foram encontradas diferenças significativas na motilidade progressiva, vigor e integridade de membrana celular entre tratamentos. Entretanto, espermatozoides cultivados com secreções de células do oviduto apresentaram maior percentagem de motilidade total às três horas de cultivo, em comparação a espermatozoides cultivados na presença de heparina. Conclusão: as secreções de células do oviduto não afetam a motilidade progressiva nem o vigor ou a integridade da membrana citoplasmática dos espermatozoides, porém prolongam a motilidade dessas células.Palavras-chave: capacitação espermática, células do oviduto, meio condicionado.

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Section: Resultsunclassified

“…Além disso, tem sido descrito que o fluido do oviduto afeta a função espermática dependendo da região do oviduto e do estágio do ciclo estral no qual o fluido ou as células são obtidas [12,34].…”

Section: Resultsunclassified

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

et al. 2015

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“…2008). Functional studies indicate that oviduct specific glycoproteins secreted from oviductal epithelium have a direct effect on maintaining motility, viability, acrosomal integrity (Kumaresan et al. 2005, 2006a; Imam et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Oviductal Proteins on Structural and Functional Characteristics of Cryopreserved Sperm in Murrah Buffaloes

Imam

Mr²,

Kumar³

et al. 2010

Reproduction in Domestic Animals

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The present study was undertaken to elucidate the effect of non-luteal oviductal proteins on sperm characteristics in Murrah buffaloes. Oviducts from healthy buffaloes were collected immediately after slaughter and the oestrous cycle phase was determined as either luteal or non-luteal based on ovarian morphology. Non-luteal oviducts (n = 80) were flushed from the isthmic end of the oviduct with PBS, fluid was centrifuged at 10,000 g at 4 degrees C for 20 min and then dialysed and clarified. The supernatant obtained was lyophilized to concentrate the protein and stored at -20 degrees C till use. Sixteen good quality ejaculates from four Murrah buffalo bulls were collected using an artificial vagina. After fresh semen analysis, each ejaculate was split into two parts and extended in Tris-citrate-egg yolk glycerol dilutor. Part I of the split ejaculate was treated with non-luteal oviductal proteins at the dose rate of 1 mg/ml of diluted semen, while part II remained as control. The extended semen was equilibrated for 4 h at 5 degrees C, filled in 0.5 ml French straws, exposed to LN(2) vapour, plunged into LN(2) and then stored at -196 degrees C. The equilibrated and frozen-thawed semen was evaluated for sperm motility, viability, acrosomal integrity, cervical mucus penetration test and hypo-osmotic sperm swelling test (HOST). In frozen-thawed semen, the percentage of sperm motility, viability and acrosomal integrity was significantly (p < 0.05) higher in the treatment group compared to the control group. The incorporation of non-luteal oviductal proteins in the extender increased the ability of sperm to penetrate cervical mucus both after equilibration and the freeze-thaw process. Similarly, the proportion of sperm with intact plasma membrane, as revealed by HOST values, was also significantly (p < 0.05) higher in the treatment group (32.6%) than the control group (27%) in frozen-thawed semen. It was inferred that incorporation of non-luteal whole oviductal fluid proteins improved the sperm quality in frozen-thawed semen in Murrah buffaloes.

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“…The extensive use of AI has enabled an accelerated rate of genetic selection and improvement in animal production, especially in dairy cattle (Medeiros et al, 2002); buct etawah (Ariantie et al, 2013); goat (Bezerra et al, 2011). Aplication of AI with frozen thawed semen is limited in buffalo due to poor freezability of buffalo bull spermatozoa compared to cattle bull spermatozoa (Kumaresan et al, 2005). The main hindrance to utilize cryopreserved buffalo bull semen is the damage of spermatozoa plasma membrane during cryopreservation and thawing, which reduces post-thaw spermatozoa motility and viability (Holt, 2000).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

et al. 2017

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Aceh swamp buffaloes (Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes after thawing. This study used completely a factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L 0 ), 60 mM (L 60 ), 120 mM (L 120 ) and glycerol 3% (G 3 ), 5% (G 5 ), 7% (G 7 ) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L 120 G 7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo (B. bubalis) after thawing. The percentage of sperm motility L 120 G 7 (42.60 ± 1.14); viability L 120 G 7 (55.00 ± 0.71); acrosome integrity L 120 G 7 (52.00 ± 0.71); and plasma membrane integrity L 120 G 7 (53.20 ± 1.48). The combination of lactose cryoprotectants 120 mM (L 120 ) and glycerol 7% (G 7 ) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

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Modulation of post-thaw sperm functions with oviductal proteins in buffaloes

Cited by 54 publications

References 24 publications

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

Effect of Oviductal Proteins on Structural and Functional Characteristics of Cryopreserved Sperm in Murrah Buffaloes

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

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