Modulation of plant architecture by the miR156f–OsSPL7–OsGH3.8 pathway in rice

Dai, Zhengfa; Wang, Jiang; Yang, Xiaofang; Lü, Huan; Xing, Miao; Shi, Zhenying

doi:10.1093/jxb/ery273

Cited by 81 publications

(66 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR156fh is in group I, and miR156l is in group II (Supplementary Figure S5). Transgenic OX156 lines overexpressing miR156h showed dwarfish phenotypes that were similar to previous reports (Supplementary Figure S6; Xie et al, 2006;Dai et al, 2018;Liu et al, 2019). In contrast to MIM156-3p, which exhibited enhanced blast resistance, OX156 showed enhanced susceptibility, which may be due to the down-regulation of SPL14 and WRKY45 (Figure 7).…”

Section: Discussionsupporting

confidence: 86%

Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Zhang

Zheng

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 86%

Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Zhang

Zheng

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Previous studies confirmed the reliability of the following high-confidence miRNA target pairs, including osa-miR156- OsSPL7 [ 15 ], miR529- SPL7 [ 16 ], miR172- SHAT1 [ 17 ], miR164- OMTN4 [ 18 ], miR168- AGO1 [ 19 ], and miR159- GAMYBL2 [ 20 ]. LOC_Os02g53620, LOC_Os03g07880, LOC_Os03g44540, and LOC_Os12g42400, as high-confidence target genes of osa-miR169, were members of the NF-YA family genes.…”

Section: Resultsmentioning

confidence: 72%

“…In our study, both miR156 and miR529 were downregulated and their common target OsSPL7 was upregulated under the heat stress in the rice post-meiosis panicle. These might be disadvantageous to spikelet development because downregulation of osa-miR156 and overexpression OsSPL7 would lead to decreased tillers, smaller panicle, lower fertility, and eventually reduced rice yield [ 15 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring Heat-Response Mechanisms of MicroRNAs Based on Microarray Data of Rice Post-meiosis Panicle

Peng

Zhang

Liu

et al. 2020

International Journal of Genomics

View full text Add to dashboard Cite

To explore heat response mechanisms of mircoRNAs (miRNAs) in rice post-meiosis panicle, microarray analysis was performed on RNA isolated from rice post-meiosis panicles which were treated at 40°C for 0 min, 10 min, 20 min, 60 min, and 2 h. By integrating paired differentially expressed (DE) miRNAs and mRNA expression profiles, we found that the expression levels of 29 DE-miRNA families were negatively correlated to their 178 DE-target genes. Further analysis showed that the majority of miRNAs in 29 DE-miRNA families resisted the heat stress by downregulating their target genes and a time lag existed between expression of miRNAs and their target genes. Then, GO-Slim classification and functional identification of these 178 target genes showed that (1) miRNAs were mainly involved in a series of basic biological processes even under heat conditions; (2) some miRNAs might play important roles in the heat resistance (such as osa-miR164, osa-miR166, osa-miR169, osa-miR319, osa-miR390, osa-miR395, and osa-miR399); (3) osa-miR172 might play important roles in protecting the rice panicle under the heat stress, but osa-miR437, osa-miR418, osa-miR164, miR156, and miR529 might negatively affect rice fertility and panicle flower; and (4) osa-miR414 might inhibit the flowering gene expression by downregulation of LOC_Os 05g51830 to delay the heading of rice. Finally, a heat-induced miRNA-PPI (protein-protein interaction) network was constructed, and three miRNA coregulatory modules were discovered.

show abstract

“…1), which was consistent with previous study (Guo et al 2013a). Generally, rice plants with more tillers tends to exhibit dwarf phenotype (Ishikawa et al 2005; Qi et al 2011; Dai et al 2018). In rice, overexpressing genes encoding GA2-oxidase increases tiller numbers but inhibits stem elongation, which is coupled with GA deficiency (Lo et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Rice transcription factor OsMADS57 regulates plant height by modulating gibberellin catabolism

Chu

et al. 2019

Rice

View full text Add to dashboard Cite

Background The MADS-box transcription factors mainly function in floral organ organogenesis and identity specification. Few research on their roles in vegetative growth has been reported. Results Here we investigated the functions of OsMADS57 in plant vegetative growth in rice ( Oryza sativa ). Knockdown of OsMADS57 reduced the plant height, internode elongation and panicle exsertion in rice plants. Further study showed that the cell length was remarkably reduced in the uppermost internode in OsMADS57 knockdown plants at maturity. Moreover, OsMADS57 knockdown plants were more sensitive to gibberellic acid (GA 3 ), and contained less bioactive GA 3 than wild-type plants, which implied that OsMADS57 is involved in gibberellin (GA) pathway. Expectedly, the transcript levels of OsGA2ox3 , encoding GAs deactivated enzyme, were significantly enhanced in OsMADS57 knockdown plants. The level of EUI1 transcripts involved in GA deactivation was also increased in OsMADS57 knockdown plants. More importantly, dual-luciferase reporter assay and electrophoretic mobility shift assay showed that OsMADS57 directly regulates the transcription of OsGA2ox3 as well as EUI1 through binding to the CArG-box motifs in their promoter regions. In addition, OsMADS57 also modulated the expression of multiple genes involved in GA metabolism or GA signaling pathway, indicating the key and complex regulatory role of OsMADS57 in GA pathway in rice. Conclusions These results indicated that OsMADS57 acts as an important transcriptional regulator that regulates stem elongation and panicle exsertion in rice via GA-mediated regulatory pathway. Electronic supplementary material The online version of this article (10.1186/s12284-019-0298-6) contains supplementary material, which is available to authorized users.

show abstract

Modulation of plant architecture by the miR156f–OsSPL7–OsGH3.8 pathway in rice

Abstract: miR156f modulates rice plant architecture by direct binding to the OsGH3.8 promoter through its target OsSPL7, to allow crosstalk with the auxin signaling pathway.

Cited by 81 publications

References 68 publications

Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Exploring Heat-Response Mechanisms of MicroRNAs Based on Microarray Data of Rice Post-meiosis Panicle

Rice transcription factor OsMADS57 regulates plant height by modulating gibberellin catabolism

Contact Info

Product

Resources

About