Modulation of piezoelectric properties in electrospun PLLA nanofibers for application-specific self-powered stem cell culture platforms

Tai, Youyi; Yang, S.Q.; Yu, Sooyoun; Banerjee, Aihik; Myung, Nosang V.; Nam, Jin

doi:10.1016/j.nanoen.2021.106444

Cited by 71 publications

(66 citation statements)

References 53 publications

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“…Previous work also demonstrated that the surface charge generated by piezoelectric scaffolds had potential to control protein adsorption and consequently cellular viability . The cell viability of 1.0PANI-MoS 2 /PVDF was greatly higher than that of PVDF under the same ultrasonic conditions, which was due to the enhanced output performance. , …”

Section: Resultsmentioning

confidence: 92%

“…46 The cell viability of 1.0PANI-MoS 2 /PVDF was greatly higher than that of PVDF under the same ultrasonic conditions, which was due to the enhanced output performance. 47,48 Figure 9b shows the CCK-8 assay of cells on different scaffolds, with the purpose of quantitatively analyzing cell proliferation. 49,50 Ultrasound was an acoustic intensity wave, which might generate mechanical stimulation for cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

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Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Gao

Peng

et al. 2021

ACS Appl. Nano Mater.

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Piezoelectric polyvinylidene fluoride (PVDF) provided an opportunity for non-invasive in situ electrical stimulation of cell behavior, yet its electroactive β phase was difficult to obtain due to its instability in the molten state. Herein, polyaniline (PANI) protrusions were in situ oxidation-polymerized on molybdenum disulfide (MoS2) nanosheets (PANI-MoS2). Then, PANI-MoS2 was introduced into laser additive-manufactured PVDF scaffolds. On the one hand, PANI protrusions produced steric hindrance between adjacent MoS2 nanosheets and inhibited the stacking and aggregating of MoS2. On the other hand, PANI-MoS2 could serve as a platform to achieve interfacial polarization locking. Specifically, Mo–S dipoles in MoS2 and π electron clouds over the N atom in PANI locked −CH2 dipoles in PVDF through electrostatic and hydrogen bond interactions, respectively, which forced −CH2 to align perpendicularly to the basal plane of MoS2 and bialy to one side of the PVDF main chain, thereby forming a full-reverse planar zigzag configuration of the polarized β phase and maintaining its stable existence. The results demonstrated that the β phase of the scaffolds was significantly increased from 43 to 90%, which resulted in an enhanced electrical output performance. The improved electrical output greatly promoted osteoblast-like cell proliferation and differentiation. Furthermore, owing to the pulling-out effect of MoS2 and improved interfacial stress transfer between MoS2 and the polymer matrix, the mechanical properties of scaffolds were also enhanced. These findings suggested that the piezoelectric scaffolds had great potential in bone tissue engineering.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Gao

Peng

et al. 2021

ACS Appl. Nano Mater.

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“…In a recent study, we systematically examined the differentiation behaviors of human NSCs and MSCs cultured on the electrospun PLLA scaffolds with either high orthogonal piezoelectricity or high shear piezoelectricity, depending on the annealing temperature as described earlier [155]. A significant difference in cell differentiation efficiency was observed where NSCs cultured on high orthogonal piezoelectric PLLA scaffolds exhibited greater neuronal differentiation as compared to those cells cultured on high shear piezoelectric PLLA scaffolds (Figure 4a-f).…”

Section: Piezoelectric Polymeric Scaffoldsmentioning

confidence: 92%

“…PLLA has been previously shown to exhibit the highest value of the shear piezoelectric coefficient of d 14 at approximately 12 pC/N [169]. We recently found that the shear piezoelectric property of electrospun PLLA nanofibers can be further tuned by annealing the samples using different temperature regimens [155]. When the annealing temperature was above the glass transition temperature of PLLA (65 • C), the shear piezoelectricity was significantly improved due to the increase in the electroactive α' phase.…”

Section: Piezoelectric Polymeric Scaffoldsmentioning

confidence: 99%

Development and Utilization of Multifunctional Polymeric Scaffolds for the Regulation of Physical Cellular Microenvironments

Tai¹,

Banerjee²,

Goodrich³

et al. 2021

Polymers

Self Cite

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Polymeric biomaterials exhibit excellent physicochemical characteristics as a scaffold for cell and tissue engineering applications. Chemical modification of the polymers has been the primary mode of functionalization to enhance biocompatibility and regulate cellular behaviors such as cell adhesion, proliferation, differentiation, and maturation. Due to the complexity of the in vivo cellular microenvironments, however, chemical functionalization alone is usually insufficient to develop functionally mature cells/tissues. Therefore, the multifunctional polymeric scaffolds that enable electrical, mechanical, and/or magnetic stimulation to the cells, have gained research interest in the past decade. Such multifunctional scaffolds are often combined with exogenous stimuli to further enhance the tissue and cell behaviors by dynamically controlling the microenvironments of the cells. Significantly improved cell proliferation and differentiation, as well as tissue functionalities, are frequently observed by applying extrinsic physical stimuli on functional polymeric scaffold systems. In this regard, the present paper discusses the current state-of-the-art functionalized polymeric scaffolds, with an emphasis on electrospun fibers, that modulate the physical cell niche to direct cellular behaviors and subsequent functional tissue development. We will also highlight the incorporation of the extrinsic stimuli to augment or activate the functionalized polymeric scaffold system to dynamically stimulate the cells.

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“…Nanomaterials, particularly nanofibers, are the subject of intense studies because of their potential uses in medicine, electronics, and catalysis 1 – 3 . Indeed, nanofibers serve as architectural elements in cell and tissue scaffolds 4 – 6 and are a basis for artificial skins and intelligent textiles 7 , 8 . In addition, they are also an efficient delivery system for bioactive molecules in therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

A new versatile x–y–z electrospinning equipment for nanofiber synthesis in both far and near field

Calzado-Delgado

Guerrero‐Pérez

Yeung

2022

Sci Rep

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This work describes a versatile electrospinning equipment with rapid, independent, and precise x–y–z movements for large-area depositions of electrospun fibers, direct writing or assembly of fibers into sub-millimeter and micron-sized patterns, and printing of 3D micro- and nanostructures. Its versatility is demonstrated thought the preparation of multilayered functional nanofibers for wound healing, nanofiber mesh for particle filtration, high-aspect ratio printed lines, and freestanding aligned nanofibers.

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Modulation of piezoelectric properties in electrospun PLLA nanofibers for application-specific self-powered stem cell culture platforms

Cited by 71 publications

References 53 publications

Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Development and Utilization of Multifunctional Polymeric Scaffolds for the Regulation of Physical Cellular Microenvironments

A new versatile x–y–z electrospinning equipment for nanofiber synthesis in both far and near field

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Modulation of piezoelectric properties in electrospun PLLA nanofibers for application-specific self-powered stem cell culture platforms

Cited by 71 publications

References 53 publications

Polyaniline Protrusions on MoS2 Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Polyaniline Protrusions on MoS2 Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Development and Utilization of Multifunctional Polymeric Scaffolds for the Regulation of Physical Cellular Microenvironments

A new versatile x–y–z electrospinning equipment for nanofiber synthesis in both far and near field

Contact Info

Product

Resources

About

Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation

Polyaniline Protrusions on MoS₂ Nanosheets for PVDF Scaffolds with Improved Electrical Stimulation