Modulation of peroxisomal compartment by Epstein-Barr virus

Indari, Omkar; Rani, Annu; Baral, Budhadev; Ergün, Süleyman; Bala, Kiran; Karnati, Srikanth; Jha, Hem Chandra

doi:10.1016/j.micpath.2022.105946

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Growing evidence suggests lipid metabolism reprogramming may have a role in aggressive cancer properties 60 . A study from our lab also shows the accumulation of lipids in red blood cells after infection with EBV and Plasmodium falciparum 61 . Furthermore, the study revealed that to promote malignant behaviors such as cell death resistance and replicative immortality enablement, cancer cells boost de novo synthesis to better use lipids in energy metabolism 62 .…”

Section: Discussionmentioning

confidence: 87%

“…60 A study from our lab also shows the accumulation of lipids in red blood cells after infection with EBV and Plasmodium falciparum. 61 Furthermore, the study revealed that to promote malignant behaviors such as cell death resistance and replicative immortality enablement, cancer cells boost de novo synthesis to better use lipids in energy metabolism. 62 A study by Liu et al 63 shows that high-density lipoprotein cholesterol improved the nasopharyngeal carcinoma (NPC) cells' capacity for proliferation, migration, colony formation, and chemoresistance; nevertheless, a greater level of HDL cholesterol was discovered to be a poor predictor of NPC patients' survival rates.…”

Section: Connectome Analysis Of Hit Molecules Depicts the Cholesterol...mentioning

confidence: 99%

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Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co‐infected gastric epithelial cells

Kashyap,

Tanwar,

Rani

et al. 2024

J Raman Spectroscopy

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Helicobacter pylori and Epstein–Barr Virus (EBV) are Group 1 carcinogens that can enhance gastric cancer progression. Bioactive substances extracted from plants can be effective therapeutic agents in cancer treatment. For example, Withania somnifera extract‐WSE reduces the Gankyrin oncoprotein, which is upregulated in the presence of H. pylori and EBV. The various biochemical and metabolic changes upon 24 hrs post‐infection followed by W. somnifera extract (WSE) treatment on gastric epithelial cells (AGS) can be studied using spectroscopic techniques. In the biomedical sciences, Raman and NMR spectroscopy have been extensively employed to interpret cellular alterations contributing to the onset of infection and the severity of gastric cancer. More specifically, alterations in cellular biochemical homeostasis are linked to the moieties of cholesterol, collagen, choline, carbohydrate, lipids, tyrosine, and phenylalanine. Further, we have found significantly elevated FWHM for carbohydrates, tumor associated protein, collagen, cholesterol, and cholesterol ester in the co‐infection model. We also looked into the potential correlation between these molecules using molecular network analysis and found several related factors that can be modulated through biomolecular levels. These molecules are crucial in several physiological functions, including cell division, cell proliferation, apoptosis, necrosis, cell migration, and lipid transport. Our study paves the pathway to study H. pylori and EBV co‐infection in human gastric epithelial cells and the therapeutic interventions of WSE in this scenario and highlights specific biomolecular alterations, which can be focused for further mechanistic investigations.

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Section: Discussionmentioning

confidence: 87%

Section: Connectome Analysis Of Hit Molecules Depicts the Cholesterol...mentioning

confidence: 99%

Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co‐infected gastric epithelial cells

Kashyap,

Tanwar,

Rani

et al. 2024

J Raman Spectroscopy

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“…After 6 h of incubation, cell pellets were collected. RNA isolation was performed using TRIzol reagent (Sigma, USA) [ 39 ]. Two micrograms of RNA was used to prepare cDNA by TAKARA cDNA synthesis kit (Takara, Japan).…”

Section: Methodsmentioning

confidence: 99%

Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity

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Thakur,

Khan

et al. 2024

J Mater Sci: Mater Med

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Multi-walled Carbon Nanotubes (MWCNTs) are inert structures with high aspect ratios that are widely used as vehicles for targeted drug delivery in cancer and many other diseases. They are largely non-toxic in nature however, when cells are exposed to these nanotubes for prolonged durations or at high concentrations, they show certain adverse effects. These include cytotoxicity, inflammation, generation of oxidative stress, and genotoxicity among others. To combat such adverse effects, various moieties can be attached to the surface of these nanotubes. Curcumin is a known anti-inflammatory, antioxidant and cytoprotective compound derived from a medicinal plant called Curcuma longa. In this study, we have synthesized and characterized Curcumin coated-lysine functionalized MWCNTs and further evaluated the cytoprotective, anti-inflammatory, antioxidant and antiapoptotic effect of Curcumin coating on the surface of MWCNTs. The results show a significant decrease in the level of inflammatory molecules like IL-6, IL-8, IL-1β, TNFα and NFκB in cells exposed to Curcumin-coated MWCNTs as compared to the uncoated ones at both transcript and protein levels. Further, compared to the uncoated samples, there is a reduction in ROS production and upregulation of antioxidant enzyme-Catalase in the cells treated with Curcumin-coated MWCNTs. Curcumin coating also helped in recovery of mitochondrial membrane potential in the cells exposed to MWCNTs. Lastly, cells exposed to Curcumin-coated MWCNTs showed reduced cell death as compared to the ones exposed to uncoated MWCNTs. Our findings suggest that coating of Curcumin on the surface of MWCNTs reduces its ability to cause inflammation, oxidative stress, and cell death. Graphical Abstract (a) Synthesis of Curcumin-coated-Lysine-functionalized MWCNTs. (b) Flow of research depicting experimental groups and studies performed along with the underlying techniques used.

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“…These proteins play roles in controlling viral gene expression, viral DNA replication, production of virion structural components, and regulation of the host immune response. This virus primarily infects epithelial cells and B lymphocytes but can also target other types of cells (Indari et al 2021b , 2022d ). The route of transmission comes into close contact with the oropharyngeal secretions of an infected individual.…”

Section: Introductionmentioning

confidence: 99%

Awakening the sleeping giant: Epstein–Barr virus reactivation by biological agents

Indari,

Ghosh,

Bal

et al. 2024

Pathogens and Disease

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Epstein Barr Virus (EBV) may cause harm in immunocompromised conditions or on stress stimuli. Various chemical agents have been utilized to induce the lytic cycle in EBV infected cells. However, apart from chemical agents and external stress stimuli, certain infectious agents may reactivate the EBV. In addition, the acute infection of other pathogens may provide suitable conditions for EBV to thrive more and planting the roots for EBV associated pathologies. Various bacteria such as periodontal pathogens like Aggregatibacter, Helicobacter pylori, etc. have shown to induce EBV reactivation either by triggering host cells directly or indirectly. Viruses such as Human Simplex virus-1 (HSV) induce EBV reactivation by HSV US3 kinase while other viruses such as HIV, hepatitis virus and even novel SARS-CoV-2 have also been reported to cause EBV reactivation. The eukaryotic pathogens such as Plasmodium falciparum and Aspergillus flavus also can reactivate EBV either by surface protein interaction of as an impact of aflatoxin respectively. To highlight the underexplored niche of EBV reactivation by biological agents, we have comprehensively presented the related information in this review. This may help to shedding the light on the research gaps as well as to unveil yet unexplored mechanisms of EBV reactivation.

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Modulation of peroxisomal compartment by Epstein-Barr virus

Cited by 7 publications

References 22 publications

Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co‐infected gastric epithelial cells

Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co‐infected gastric epithelial cells

Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity

Awakening the sleeping giant: Epstein–Barr virus reactivation by biological agents

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