Modulation of nucleotide pyrophosphatase in plasmacytoma cells

Dexamethasone Enhances ATP-Induced Inflammatory Responses in Endothelial Cells

“…Our findings suggest that in contrast glucocorticoids contribute to a positive feedback loop that amplifies inflammatory responses initiated by ATP, which may result in enhanced migration and activation of inflammatory cells to injured or infected tissue. This proinflammatory mechanism may be dampened by glucocorticoid induction of ectonucleotide pyrophosphatase/ phosphodiesterase NPP1, resulting in extracellular nucleotide hydrolysis and recycling (Rebbe and Hickman, 1991;Goding et al, 2003). In addition, ATP stimulated the induction of TNFAIP3 mRNA (Fig.…”

Identification of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity associated with the mouse plasma cell differentiation antigen PC-1.

“…In addition, this antiserum was used to analyze immunoblots of cell lysates from MOPC 315 cell lines known to have different levels of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity. We have shown that 315/P variant cells have a marked increase in nucleotide pyrophosphatase specific activity compared with wild-type MOPC 315/J cells (12), and levels of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity can be modulated in MOPC 315 cells with the glucocorticoid dexamethasone (14). The anti-PC-1 antiserum detected two protein bands (Fig.…”

“…The 315/P cell line is a nonsecretory variant previously shown to have high levels of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity (12). In some experiments, both cells lines were incubated with dexamethasone since, as has been described for other cell lines (13), we have found this corticosteroid increases specific activity of nucleotide pyrophosphatase/alkaline phosphodiesterase I in MOPC 315 cells (14). Because they express such high levels of nucleotide pyrophosphatase/alkaline phosphodiesterase I, 315/P cells were used as a source for the purification of this membrane protein.…”

“…Because the variant 315/P cells express high levels of nucleotide pyrophosphatase/alkaline phosphodiesterase I (12), they were used as a source for mRNA. Also, these cells were incubated in 200 nM dexamethasone for 48 hr prior to extraction, since this corticosteroid further increases enzymatic specific activity (14). Extraction of total RNA (20), isolation of poly(A)+ RNA (21), and synthesis of double-stranded cDNA (22) were done by standard methods.…”

Activity of the astrocytes' adenosine signaling system components in model of traumatic brain injury in vivo and in vitro

“…Ekspresija e-NPPaze2 u CNS-u je ograničena na ćelije horoidnog pleksusa, cerebrospinalnu tečnost [152] i nezrele oligodendrocite [147,153]. ENPPaza3 je detektovan na glijskim ćelijama tokom razvića, zatim u prekursorima ali ne i u zrelim astrocitima [154].…”