Modulation of noradrenergic and serotonergic transmission by noxious stimuli and intrathecal morphine differs in the dorsal raphe nucleus of anesthetized rat: in vivo voltammetric studies

Chuma, Toichiro; Taguchi, Kyoji; Kato, Masaaki; Abe, Kenji; Utsunomiya, Iku; Miyamoto, Ken‐ichi; Miyatake, Tadashi

doi:10.1016/s0168-0102(02)00084-6

Cited by 10 publications

(2 citation statements)

References 41 publications

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“…CSF‐cNs have been identified in hypothalamic nuclei where they might take part in neuroendocrine regulations (Vígh & Vígh‐Teichmann, 1998; Xiao et al 2005). They have also been observed in the dorsal raphe nucleus, a brain region involved in pain modulation and anti‐nociception function (Wang & Nakai, 1994; Chuma et al 2002). The largest number of CSF‐cNs is found in the region surrounding the central canal (cc) of the spinal cord, a plastic zone capable of limited endogenous repairs in the event of spinal injuries (Mothe & Tator, 2005; Meletis et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Properties of subependymal cerebrospinal fluid contacting neurones in the dorsal vagal complex of the mouse brainstem

Orts-Del’Immagine

Wanaverbecq

Tardivel

et al. 2012

The Journal of Physiology

122

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Key points• The brainstem is a major site of integration for autonomic information from neurones, blood and cerebrospinal fluid (CSF). Signals exchange from the CSF is limited by the ependymocytes forming the brain cavities. However neurones contacting the CSF (CSF-cNs) are thought to integrate those signals.• Using immunohistochemical and electrophysiological approaches, we characterise in the brainstem, subependymal CSF-cNs projecting a process ending in the central canal with a protrusion and expressing a 'transient receptor potential' (TRP) channel subtype suggested to act as chemoor mechanoreceptors: the polycystin kidney disease 2-like 1 channels (PKD2L1).• CSF-cNs receive exclusively inhibitory synaptic inputs and express functional channels presenting all properties of PKD2L1: cationic non-selective, large conductance and modulated by extracellular pH and osmolarity.• Because medullar CSF-cNs are strategically positioned between CSF and neuronal parenchyma, we hypothesise that they could play a role in the regulation of homeostasis by integrating CSF signals.Abstract Cerebrospinal fluid (CSF) contacting neurones have been observed in various brain regions such as the hypothalamus, the dorsal nucleus of the raphe and around the central canal (cc) of the spinal cord but their functional role remains unclear. At the level of the spinal cord, subependymal cerebrospinal fluid contacting neurones (S-CSF-cNs) present a peculiar morphology with a soma close to the ependymal layer, a process projecting towards the cc and ending with a bud and a cilium. These neurones were recently shown to express polycystin kidney disease 2-like 1 (PKD2L1 or TRPP3) channels that are members of the polycystin subtype of the transient receptor potential (TRP) channel superfamily and that have been proposed as either chemoor mechanoreceptors in several tissues. Using immunohistological techniques and whole-cell electrophysiological recordings in brain slices obtained from PKD2L1:EGFP transgenic adult mice, we looked for and determined the functional properties of S-CSF-cNs in the dorsal vagal complex (DVC), a hindbrain structure controlling autonomic functions such as blood pressure, energy balance and food intake. Here, we demonstrate that S-CSF-cNs received GABAergic and/or glycinergic synaptic entries and were also characterised by the presence of non-selective cationic channels of large conductance that could be detected even under whole-cell configuration. The channel activity was not affected by Psalmopoeus cambridgei toxin 1, a blocker of acid sensing ion channels (ASICs), but was blocked by amiloride and by a strong extracellular acidification. In contrast, extracellular alkalinisation and hypo-osmotic shocks increased channel activity. Based on these properties, we suggest that the single-channel activity recorded in medullar S-CSF-cNs is carried by PKD2L1 channels. Our study therefore reinforces the idea that PKD2L1 is a marker A. Orts-Del'Immagine and N. Wanaverbecq contributed equally to the work.

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Section: Introductionmentioning

confidence: 99%

Properties of subependymal cerebrospinal fluid contacting neurones in the dorsal vagal complex of the mouse brainstem

Orts-Del’Immagine

Wanaverbecq

Tardivel

et al. 2012

The Journal of Physiology

122

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“…These regions include the locus coeruleus nucleus, the solitary tract nucleus, the raphe magnus nucleus, the substantia nigra, the thalamo-central medial nucleus, the parafascicular nucleus, the gigantocellular reticular nucleus, the preoptic area and the cortex. Many studies have indicated that the neurons in the mesencephalon region ventral to the aqueduct have many physiological functions including thermoregulation [ 25 ], sleep [ 26 - 28 ], cardiovascular functions [ 29 ], hormone-endocrine functions [ 30 , 31 ], multiple arousal systems [ 32 ], obesity [ 33 ], anxiety and depression [ 34 , 35 ], conditioned-fear and stress [ 36 ], sexual behaviors [ 37 ] and mood disorders [ 38 ] and are also important in pain modulation and anti-nociceptive function [ 39 , 40 ]. Zhang et al [ 7 ] indicated that the mesencephalon region ventral to the aqueduct was a locus which had the largest number of distal CSF contacting neurons and the most concentrated distribution.…”

Section: Discussionmentioning

confidence: 99%

Expression of TRPM8 in the distal cerebrospinal fluid-contacting neurons in the brain mesencephalon of rats

Yang

Zhang

et al. 2009

Fluids Barriers CNS

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Background: It has been shown that distal cerebrospinal fluid-contacting neurons (dCSF-CNs) exist near the ventral midline of the midbrain aqueduct and also in the grey matter of the inferior third ventricle and the fourth ventricle floor in the superior segment of the pons. The dCSF-CNs communicate between the cerebrospinal fluid (CSF) and the brain parenchyma and may participate in the transduction and regulation of pain signals. The cold sensation receptor channel, TRPM8 is involved in analgesia for neuropathic pain, but whether the TRPM8 receptor exists on dCSF-CNs remains unknown. However, there is preliminary evidence that TRPM8 is expressed in dCSF-CNs and may participate in the transmission and regulation of sensory information between brain parenchyma and cerebrospinal fluid (CSF) in rats.

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Effects of intrathecal BAM22 on noxious stimulus-evoked c-fos expression in the rat spinal dorsal horn

Zeng

Huang

2004

Brain Research

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Modulation of noradrenergic and serotonergic transmission by noxious stimuli and intrathecal morphine differs in the dorsal raphe nucleus of anesthetized rat: in vivo voltammetric studies

Cited by 10 publications

References 41 publications

Properties of subependymal cerebrospinal fluid contacting neurones in the dorsal vagal complex of the mouse brainstem

Properties of subependymal cerebrospinal fluid contacting neurones in the dorsal vagal complex of the mouse brainstem

Expression of TRPM8 in the distal cerebrospinal fluid-contacting neurons in the brain mesencephalon of rats

Effects of intrathecal BAM22 on noxious stimulus-evoked c-fos expression in the rat spinal dorsal horn

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