Modulation of natural killer cytotoxicity by muramyl dipeptide and trehalose dimycolate incorporated in squalane droplets

Kn, Masihi; Lange, W; Rohde-Schulz, Beate

doi:10.1007/bf00199828

Cited by 6 publications

(3 citation statements)

References 28 publications

(25 reference statements)

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“…Enhancement of NK and ADCC re-sponse was more rapid (but also more transient) in mice with constitutively weak NK activity (C57B1) than in strong reactors (CBA). Strain-related responsiveness has been recorded with MDP, a synthetic analog of peptidoglycans [14,21,23]: C57B1 mice were resistant to its stimulating effect, whereas the other strains responded. Similar kinetics of NK modulation as found by us in PGM treated CBA mice has been recently reported in DBA mice injected with MDP [13].…”

Section: Discussionmentioning

confidence: 99%

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In vivo and in vitro modulation of NK and ADCC activities of mouse spleen cells by peptidoglycan monomer (PGM)

et al. 1989

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The ability of peptidoglycan monomer (PGM), an immunomodulator obtained from Brevibacterium divaricatum, to modulate NK and ADCC activities of spleen cells was tested in mice with constitutively weak (C57B1) or strong NK activity (CBA, C3H). In weak reactors, i.v. injection of PGM enhanced the NK and ADCC activity as effectively as a known stimulator, poly I.C, and the dynamics of the response was comparable. In strong reactors, PGM caused two peaks of the ADCC response, and one peak of NK stimulation (after an initial decline), whereas poly I.C caused more or less persistent stimulation of both activities. Incubation of spleen cells with PGM was generally less effective than the treatment in vivo. No alteration of NK activity was recorded at high effector-to-target ratio (E:T), and at low ones, PGM caused suppression. This was true both for weakly and strongly reactive cells. ADCC was either unchanged (CBA spleen cells), stimulated (C3H), or suppressed at high E:T and enhanced at low E:T (C57B1). PGM apparently activates an interlocked regulatory mechanism, and the final outcome probably depends on relative concentrations of regulatory and effector cells and on their per cell activities. Hence the effect varied with the time interval between treatment and assay, with strain-related constitutive reactivity, and with the E:T ratio.

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Section: Discussionmentioning

confidence: 99%

“…Since strain-related differences have been recorded whith synthetic analog of PGM, muramyl dipeptide (MDP) [14,21,23], effects of PGM were evaluated in mice with constitutively weak (C57B1) or strong (CBA, C3H) NK activity.…”

Section: Introductionmentioning

confidence: 99%

In vivo and in vitro modulation of NK and ADCC activities of mouse spleen cells by peptidoglycan monomer (PGM)

et al. 1989

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“…Only a few reports [7][8][9][10] concerning the synthesis of a novel adamantyl-amide dipeptide that consisted of an adamantyl moiety and a dipeptide characteristic for MDP were reported. Preliminary tests showed that this compound exhibits both immunomodulating and antiviral properties in several experimental models.…”

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confidence: 99%

Synthesis and Antiviral Activity of Novel Adamantylpeptides

Vranešić

Tomašić

Smerdel

et al. 1993

Helvetica Chimica Acta

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A novel type of compound that contains a peptide specific for bacterial peptidoglycans (immunomodulatory activity) and an adamantyl residue (antiviral activity) was synthesized. Thus, L-Ala-D-y GIn was linked to DLGly(Ada) yielding two diastereoisomers 3a and 3b, i.e. ambo-Gly(Ada)-L-Ala-D-y Gln (y Gln = isoglutamine-4-amino-4-carbamoylbutanoic acid; Ada = adamantyl). The diastereoisomers were easily separated by column chromatography (silica gel) and characterized, but only the use of a stereospecific enzyme led to the assignment of the configuration of the Gly(Ada) moiety in the two diastereoisomers. The cytotoxic effect and antiviral activity of the novel adamantyltripeptides 3a and 3b, observed as the cell survival and the infectivity reduction of the influenza A virus H l N l and H3N2 strains, respectively, were studied on MDCK cells in oitro. Both 3a and 3b exhibited low cytotoxicity for MDCK cells and reduced the infectivity of the tested influenza A virus strains, but to a different extent on different strain.

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The in vitro effect of new muramyl peptide derivatives on cytotoxic activity of NK (Natural Killer) cells from hamsters bearing AB Bomirski Melanoma

Sоsnowska¹,

Myśliwski²,

Dzierzbicka³

et al. 1997

Biotherapy

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The modulation of NK activity by muramyl dipeptides derivatives against Ab (amelanotic) Bomirski melanoma and human erythroleukemia K562 cells was studied in vitro. The stimulatory effect was observed for 3 of 7 muramyl dipeptides: MDP(L-Ala)C921, MDPC857 and L18-MDP(Ala) in relation to cytotoxic activity of NK cells obtained from peripheral blood and spleen of healthy and Ab Bomirski melanoma bearing hamsters. An increased of cytotoxic activity NK cells isolated from animals before and during the transplantable phase of the tumor against K562 was found. A similar stimulation was received for NK cells obtained from animals against their own melanoma cells. The most significant influence of examined MDP derivatives on the cytotoxic activity of NK cells were obtained from animals between 10 to 12 days of tumor growth. The extent of the modulation of cytotoxic activity of NK cells was dependent on its initial value both in healthy control and Ab Bomirski melanoma bearing hamsters. If natural cytotoxic activity was high the stimulatory effect of the examined MDP derivatives was only slightly expressed.

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Modulation of natural killer cytotoxicity by muramyl dipeptide and trehalose dimycolate incorporated in squalane droplets

Cited by 6 publications

References 28 publications

In vivo and in vitro modulation of NK and ADCC activities of mouse spleen cells by peptidoglycan monomer (PGM)

In vivo and in vitro modulation of NK and ADCC activities of mouse spleen cells by peptidoglycan monomer (PGM)

Synthesis and Antiviral Activity of Novel Adamantylpeptides

The in vitro effect of new muramyl peptide derivatives on cytotoxic activity of NK (Natural Killer) cells from hamsters bearing AB Bomirski Melanoma

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