Modulation of mucosal immunity in a murine model of food‐induced intestinal inflammation

Cardoso, Cássia Regina Primila; Teixeira, Gerlinde Agate Platais Brasil; Provinciatto, Pauline R.; Godoi, Dannielle Fernandes; Ferreira, Beatriz Rossetti; Milanezi, Cristiane M.; Ferraz, D B; Rossi, Marcos A.; Cunha, Fernando; Silva, J. S.

doi:10.1111/j.1365-2222.2007.02866.x

Cited by 43 publications

(43 citation statements)

References 40 publications

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“…For instance, we have described a similar increase in CTMCs in the jejunum of Trichinella spiralis-infected rats, an accepted model of post-infectious IBS that also courses with dysmotility. 33 Moreover, CTMC counts are increased in mice with food allergy 34,35 and the degranulation of CTMCs excites the intestinal smooth muscle in vitro. 36 Although further in deep studies are required, these observations suggest that submucosal CTMC could be taking part in the altered contractile responses observed in OVA-LPS-treated rats.…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharides Facilitate Colonic Motor Alterations Associated to the Sensitization to a Luminal Antigen in Rats

Jardí

Aguilera

Vergara

et al. 2015

J Neurogastroenterol Motil

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Background/Aims Enteric dysbiosis is a risk factor for dietary proteins-associated intestinal alterations, contributing to the development of food allergies and the symptomatology of functional gastrointestinal disorders, mainly irritable bowel syndrome (IBS). We explored if a dysbiotic-like state, simulated by intraperitoneal administration of bacterial lipopolysaccharides (LPS), facilitates the sensitization to a luminal antigen, ovalbumin (OVA), in rats. Methods Rats were exposed to oral OVA for 1 week, alone or with LPS. Thereafter, colonic histology, goblet cell density, mucosal eosinophils and mucosal mast cell (MMC) and connective tissue mast cell (CTMC) were evaluated. Colonic expression (real-time quantitative polymerase chain reaction) of interleukins, IFN-α1 and integrins was assessed to determine local immune responses. Luminal and wall adhered microbiota were characterized by fluorescence in situ hybridization. Colonic contractility (in vitro) served to assess functional changes associated to OVA and/or LPS. Results Neither OVA nor LPS, alone or combined, lead to structural alterations, except for a reduced goblet cell density in OVA-LPS-treated rats. MMC density was unaffected, while CTMC counts increased within the submucosa of OVA-LPS-treated animals. Marginal immune activation (IFN-α1 up-regulation) was observed in OVA-LPS-treated rats. LPS induced a dysbiotic-like state characterized by decreased luminal bacterial counts, with a specific loss of clostridia. LPS facilitated Clostridium spp. wall adherence, an effect prevented by OVA. Colonic contractility was altered in OVA-LPS-treated animals, showing increased basal activity and enhanced motor responses to OVA. Conclusions Changes in gut microbiota and/or direct effects of LPS might enhance/facilitate local neuroimmune responses to food antigens leading to motor alterations similar to those observed in IBS.

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Section: Discussionmentioning

confidence: 99%

Lipopolysaccharides Facilitate Colonic Motor Alterations Associated to the Sensitization to a Luminal Antigen in Rats

Jardí

Aguilera

Vergara

et al. 2015

J Neurogastroenterol Motil

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“…For the induction of food enteropathy, C57BL/6 wild-type (WT) and B KO mice (Igh-6 tm1Cgn ), obtained from Jackson Laboratories (Bar Harbor, ME, USA), were age (6-8 weeks old) and gender matched (n = 5 animals/group) and sensitized twice, 3 weeks apart, with 100 mg of peanut proteins extract (PPE) [9] in the presence (1st sensitization) or absence (2nd sensitization) of 1 mg of aluminum hydroxide by subcutaneous injection. One week after the last sensitization, mice were fed peanut seeds ad libitum for 30 days uninterruptedly, in the absence of the conventional chow (S + Peanut group).…”

Section: Mice Sensitization and Induction Of Food Enteropathymentioning

confidence: 99%

“…Responses generated to orally administered antigens have local and systemic features that distinguish them from other immunological reactions [8] and breakdown of mucosal tolerance to fed antigens can lead to the development of enteropathies [9] that require interactions between non-degraded proteins and gut-associated lymphoid tissue. The resulting active immunity against such non-pathogenic matters can lead to hypersensitivity responses and inflammatory disorders with subsequent allergic reaction [10,11], participation of IgE-producing B cells in response to activated T-helper type 2 (Th2) effector lymphocytes and cytokines like IL-4 or IL-13 [9].…”

Section: Introductionmentioning

confidence: 99%

“…The resulting active immunity against such non-pathogenic matters can lead to hypersensitivity responses and inflammatory disorders with subsequent allergic reaction [10,11], participation of IgE-producing B cells in response to activated T-helper type 2 (Th2) effector lymphocytes and cytokines like IL-4 or IL-13 [9].…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, in this work, we used B cell-deficient mice and a murine model of peanut sensitization described previously by our group [9] to examine the role played by B lymphocytes in the development of food allergy. Lastly, this study provided evidence that B cells are necessary for the induction of gut mucosal alterations and inflammatory response in the onset of allergic enteropathy.…”

Section: Introductionmentioning

confidence: 99%

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B cells are involved in the modulation of pathogenic gut immune response in food-allergic enteropathy

Cardoso¹,

Provinciatto²,

Godoi³

et al. 2008

Clinical and Experimental Immunology

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Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation

Baştan

Dileepan

et al. 2018

Journal of Leukocyte Biology

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Prevalence of food allergies in the United States is on the rise. Eosinophils are recruited to the intestinal mucosa in substantial numbers in food allergen-driven gastrointestinal (GI) inflammation. Soluble epoxide hydrolase (sEH) is known to play a pro-inflammatory role during inflammation by metabolizing anti-inflammatory epoxyeicosatrienoic acids (EETs) to pro-inflammatory diols. We investigated the role of sEH in a murine model of food allergy and evaluated the potential therapeutic effect of a highly selective sEH inhibitor (trans-4-{4-[3-(4-trifluoromethoxyphenyl)-ureido]-cyclohexyloxy}-benzoic acid [t-TUCB]). Oral exposure of mice on a soy-free diet to soy protein isolate (SPI) induced expression of intestinal sEH, increased circulating total and antigen-specific IgE levels, and caused significant weight loss. Administration of t-TUCB to SPI-challenged mice inhibited IgE levels and prevented SPI-induced weight loss. Additionally, SPI-induced GI inflammation characterized by increased recruitment of eosinophils and mast cells, elevated eotaxin 1 levels, mucus hypersecretion, and decreased epithelial junction protein expression. In t-TUCB-treated mice, eosinophilia, mast cell recruitment, and mucus secretion were significantly lower than in untreated mice and SPI-induced loss of junction protein expression was prevented to variable levels. sEH expression in eosinophils was induced by inflammatory mediators TNF-α and eotaxin-1. Treatment of eosinophils with t-TUCB significantly inhibited eosinophil migration, an effect that was mirrored by treatment with 11,12-EET, by inhibiting intracellular signaling events such as ERK (1/2) activation and eotaxin-1-induced calcium flux. These studies suggest that sEH induced by soy proteins promotes allergic responses and GI inflammation including eosinophilia and that inhibition of sEH can attenuate these responses.

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Modulation of mucosal immunity in a murine model of food‐induced intestinal inflammation

Cited by 43 publications

References 40 publications

Lipopolysaccharides Facilitate Colonic Motor Alterations Associated to the Sensitization to a Luminal Antigen in Rats

Lipopolysaccharides Facilitate Colonic Motor Alterations Associated to the Sensitization to a Luminal Antigen in Rats

B cells are involved in the modulation of pathogenic gut immune response in food-allergic enteropathy

Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation

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