Modulation of morpho-physiological attributes and in situ analysis of secondary metabolites using Raman spectroscopy in response to red and blue light exposure in Artemisia annua

Rai, Nidhi; Kumari, Sabitri; Singh, Sneha; Saha, Pajeb; Pandey, Adarsh Kumar; Pandey-Rai, Shashi

doi:10.1016/j.envexpbot.2023.105563

Cited by 4 publications

(1 citation statement)

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“…Changes in light wavelengths can shift the primary and secondary metabolism in plants, alter the plant’s metabolism and affect the accumulation of the plant’s functional products [ 17 ]. The enzymatic activities of phenylalanine ammonia-lyase, chalcone synthase, chalcone isomerase and flavonol synthase are integral components of the phenylpropanoid pathway, which leads to the biosynthesis of flavonoids and is observed to be stimulated either directly or indirectly in response to various light conditions [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Combined Elicitation with Light and Temperature on the Chlorogenic Acid Content, Total Phenolic Content and Antioxidant Activity of Berula erecta in Tissue Culture

Senekovič,

Ciringer,

Ambrožič-Dolinšek

et al. 2024

Plants

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Chlorogenic acid is one of the most prominent bioactive phenolic acids with great pharmacological, cosmetic and nutritional value. The potential of Berula erecta in tissue culture was investigated for the production of chlorogenic acid and its elicitation combined with light of different wavelengths and low temperature. The content of chlorogenic acid in the samples was determined by HPLC-UV, while the content of total phenolic compounds and the antioxidant activity of their ethanol extracts were evaluated spectrophotometrically. The highest fresh and dry biomasses were obtained in plants grown at 23 °C. This is the first study in which chlorogenic acid has been identified and quantified in Berula erecta. The highest chlorogenic acid content was 4.049 mg/g DW. It was determined in a culture grown for 28 days after the beginning of the experiment at 12 °C and under blue light. The latter also contained the highest content of total phenolic compounds, and its extracts showed the highest antioxidant activity. Berula erecta could, potentially, be suitable for the in vitro production of chlorogenic acid, although many other studies should be conducted before implementation on an industrial scale.

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Section: Introductionmentioning

confidence: 99%