Modulation of MMP-2 and TIMP-2 by low dose radiation in mice bearing S180 sarcoma

Abstract

ObjectiveTo investigate the inhibition of low dose radiation (LDR) on S180 sarcomas and its modulation of MMP-2 and TIMP-2 in mice.MethodsS180 subcutaneously implanted tumor model mice were randomly divided into two groups: control (N) and low dose radiation (LDR) groups. N mice were sacrificed after 12 h, whereas LDR mice were sacrificed after 12 (LDR-12 h), 24 (LDR-24 h), 48 (LDR-48 h), and 72 (LDR-72 h) h. Thereafter, we measured the tumor volumes. Histopathology was performed, and P-V immunohistochemistry was applied to assess MMP-2 and TIMP-2 expression.ResultsCompared with the control group, the tumor growth was significantly inhibited in the LDR groups (P < 0.05). MMP-2 expression was considerably reduced in LDR-24h (P < 0.05) and LDR-48h (P < 0.05), whereas the change of TIMP-2 was not obvious in the LDR groups (P > 0.05) in contrast to that of the control group.ConclusionLDR can effectively suppress the growth of S180 implanted tumors by reducing MMP-2, which is associated with invasion and metastasis.