Modulation of MHC class I antigen expression in adenovirus infection and transformation

Blair, G. Eric; Proffitt, J L; Zajdel, Maria E. Blair

doi:10.1017/cbo9780511525339.009

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, forty nine distinct human serotypes of adenovirus have been determined. They are non-enveloped isometric particles with icosahedral symmetry which contain a double stranded DNA genome, 36 kilobases long encoding late gene structural proteins (Blair et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Sivasubramaniam¹,

Fooks²,

Lee³

et al. 1997

Cytotechnology

View full text Add to dashboard Cite

We have used a recombinant adenovirus vector (E1-) expressing β-galactosidase to explore a novel mechanism with which to transfer genes into cells of the central nervous system (CNS). The replication-deficient adenovirus vector expressing β-galactosidase (RAd35) was propagated on a permissive helper cell line (293 cells). High level protein expression from the human cytomegalovirus immediate early promoter (hCMV IE) was obtained in a target cell population of RAd35 infected cultured neuronal and glial cell lines. Light microscopy showed that over 50% of the glial cells studied expressed β-galactosidase. Following retinoic acid treatment, RAd35 infected cell lines ND7/23, NG108 and NTera2, showed β-galactosidase expression in up to 90% of the cells. In addition, these cells showed morphological evidence of differentiation into neurons. This pattern of β-galactosidase expression was also observed in primary rat cerebella granule neuron cultures. In vivo studies were performed in Balb/c mice following direct intracranial injections of RAd35 into the brain. Cell sections showed a localised staining in the brain at the site of injection of the virus. Non-replicating adenovirus vectors are therefore highly efficient systems for delivering a transgene into brain cells. However, their broad cell tropism may limit their applications for genetic disorders in which a specific cell type is to be targeted for gene therapy. To address this problem, we have constructed adenovirus vectors which contain specific neuronal promoters and are currently assessing in vitro expression.

show abstract

Section: Introductionmentioning

confidence: 99%