Modulation of Meloxicam-Induced Changes in Gastrointestinal and Motor Activity of the Stomach by Applying Placenta Cryoextract

Chyzh

et al. 2023

Innov Biosyst Bioeng

Background. Peptic ulcer is a prevalent gastrointestinal tract disease worldwide. One of the treatment methods for the indicated pathology is trunk gastric cryodenervation (TGC), the feature of which is the preservation of the regenerative potential of n. vagus. Cryopreserved placenta extract (CPE) caught our attention among modern means capable of stimulating regeneration processes. Objective. We are aimed to characterize the state of gastric secretion after TGC and the application of СPE. Methods. TGC was performed by cryoinfusion on the front and back trunks of the abdominal part of n. vagus. Gastric secretion was studied using the H.A. Shay method. Results. On the 30th day after TGC, a statistically significant (p < 0.001) decrease in the total acidity of gastric juice by 33.7% and a decrease (p < 0.01) in the level of free acidity were observed compared to intact animals. Administration of СPE after TGC resulted in a statistically significant (p < 0.001) decrease in total acidity by 24.2 % and a statistically significant (p < 0.001) decrease in free acidity by 48.7% compared to the parameters of intact rats. This led to a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals (1.4 ± 0.03 RU) which was higher than the similar ratio observed after esomeprazole usage or TGC. Conclusions. Administration of СPE contributed to the partial regeneration of n. vagus, as indicated by an increase in the level of total acidity to 104.1 ± 4.7 RU and a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals.

Section: матеріали і методиunclassified

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Кошурба

Chyzh

et al. 2023

Innov Biosyst Bioeng

“…У лікуванні знайшли застосування препарати різних фармакологічних груп, які впливають на окремі низки етіології та патогенезу захворювання. Особливу увагу привертають дані про гастропротективну дію (противиразкову активність) вітчизняного біотехнологічного засобу з полівекторним механізмом дії -препарату кріоконсервованого екстракту плаценти (КЕП) людини, який створено фахівцями Інституту проблем кріобіології і кріомедицини Національної академії наук України (ІПКіК НАН України) [9][10][11][12].…”

Section: вступunclassified

“…Відповідно разова доза для щурів становить: (1,8 мл/70 кг) × 6,35 = 0,16 мл/кг маси тіла або відповідно 0,02 мл/100 г маси тіла щура [13,15]. Перед застосуванням препарату «Кріоцеллкріоекстракт плаценти» разову дозу (0,16 мл/кг) екстемпорально (ex tempore -за потребою) розводили у 0,9% р-ні NaCl (ПрАТ «Фармацевтична фірма «Дарниця», Україна) із розрахунку 0,1 мл 0,9% р-ну NaCl/100 г маси тіла та вводили в/м у профілактичному режимі 1 р/д упродовж 5 днів [10,11].…”

Section: матеріали та методиunclassified

Modulation of lipid peroxidation and energy metabolism in the gastric mucosa as a mechanism ofantiulcer activity of placental cryoextract in the healing of stress-induced ulcers

Кошурба

Chyzh

2022

GASTRO

Self Cite

Background. Peptic ulcer disease has a leading place in the overall structure of digestive diseases: its prevalence is 6.00–10.0 % of the population in developed countries, and mortality ranges from 6 to 9.7 per 100 thousand population. An important etiological factor of this pathology is the neuropsychological background, primarily stress, which under repeated exposure becomes the initiating factor of physiological imbalance between the elements of aggression and protection of the gastric mucosa. The purpose was to establish the mechanisms of the protective activity of placental cryoextract based on the biochemical indicators of lipid peroxidation and antioxidant protection in the gastric mucosa on a model of stress-induced erosive-ulcerative damage. Materials and methods. Studies were performed on 28 nonlinear laboratory male rats weighing 200–220 g. Stress-induced gastric ulcer was modeled under water-immobilization stress in rats according to the K.Y. Takagi et al. In the gastric mucosa, the content of reactants with thiobarbituric acid was determined spectrophotometrically by the method of T. Asakawa et al., catalase activity — spectrophotometrically by the method of M.A. Korolyuk et al., the content of reduced glutathione — spectrophotometrically by the method of E.D. Beutler et al., the level of adenyl nucleotides was determined using chromatographic method. Energy charge was calculated by D.E. Atkinson equation. Results. The prophylactic five-day use of placental cryoextract led to a decrease in the severity of stress-induced lipid peroxidation and energy imbalance in the gastric mucosa. Thus, it was found that rats who received placental cryoextract had a statistically significant (p < 0.001) increase in adenosine triphosphate content by 73.3 %, an increase in adenosine diphosphate (p < 0.001) by 37.3 % and a decrease in adenosine monophosphate (p < 0.001) by 47.6 % that led to an increase in energy charge (p < 0.001) by 35.1 % compared to rats exposed to water-immobilization stress without correction (control group). It was shown that the use of placental cryoextract led to a statistically significant (p < 0.001) increase in the antioxidant-prooxidant index by 3.1 times versus control group, which was (26.60 ± 0.96) and (8.60 ± 0.43), respectively. Conclusions. Prophylactic five-day administration of placental cryoextract leads to the restoration of balance in the system of adenyl nucleotides and, accordingly, to a statistically significant (p < 0.001) increase in the energy charge by 35.1 % compared to the control animals. Inhibition of stress-induced hyperactivation of lipid peroxidation in the gastric mucosa is one of the mechanisms of its antiulcer activity.

“…У дослідженні [15] доведено, що комбіноване нарізне введення мелоксикаму та КЕП супроводжувалось зниженням співвідношення вільної та загальної кислотності на 43,0 ± 3,0 % і статистично вірогідним (р < 0,05) зменшенням перистальтичної активності на 12,3 % відносно показників тварин, які отримували тільки мелоксикам. У попередньому дослідженні нами було виявлено здатність КЕП послаблювати гіперсекрецію шлункового соку та гіпермоторику, що виступає механізмом його гастроцитопротекторної акивності [16]. Скринінгові дослідження показали наявність цитопротекторної активності КЕП у ШКТ й на моделях ульцерогенезу, індукованого іншими НПЗЗацетилсаліциловою кислотою, ібупрофеном, індометацином і диклофенаком [17].…”

unclassified

An Experimental Study of the Cryopreserved Placenta Extract Effect On the Sodium Diclofenac Anti-Inflammatory Activity

2021

Innov Biosyst Bioeng

Self Cite

Background. As a means of correcting the ulcerogenic effect of nonsteroidal anti-inflammatory drugs, our attention was attracted by a cryopreserved extract of the human placenta, which has a multivector spectrum of biological activity. To date, there is no information about its effect on the specific activity of this class of medicines (anti-inflammatory, analgesic, etc.) in published sources. Objective. We are aimed to characterize the effect of cryopreserved placenta extract on the anti-inflammatory activity of diclofenac sodium when administered separately in a model of acute exudative inflammation. Methods. Experimental studies in vivo were conducted on 28 nonlinear male laboratory rats. The model of acute exudative inflammation was reproduced by subplantar injection of 0.1 ml of 1.0% aqueous solution of λ-karagenin into the right hind limb of rats. Cryopreserved placenta extract was administered intramuscularly at a dose of 0.16 ml/kg 60 minutes before diclofenac sodium (8 mg/kg). Results. Preventive administration of diclofenac sodium caused an antiexudative effect as early as 30 minutes after administration of λ-karagenin – its anti-inflammatory activity was 11.0%, which is 4.6 times higher than similar indicators at the same time in rats injected with placental cryoextract. At 60 minutes of observation, diclofenac sodium was comparable in anti-inflammatory activity with cryopreserved placenta extract: 28.6% and 22.2%, respectively, but at 120 and 180 minutes, diclofenac sodium exceeded the studied cryoextract in antiphlogistic effect by 1.6 times in both periods of observation. The anti-inflammatory effect of the combined separate administration of placenta cryoextract and diclofenac sodium before λ-karagenin for 30 and 60 minutes was 12.7% and 32.3%, respectively, which is comparable with analogous indicators against the background of diclofenac sodium monotherapy. However, at 120 minutes of observation, the group of combined use of placenta cryoextract and diclofenac sodium showed the greatest anti-inflammatory effect among rats of all the studied groups – 52.6%, which was 2.2 times higher than the indicators of the placenta cryoextract monotherapy group and 1.4 times lower than the indicators of the rats of the diclofenac sodium monotherapy group. Conclusions. 4 hours after administration, placental cryoextract had a suppressive effect on kinins like diclofenac sodium, and in the prostaglandin period of caragenin-induced inflammation against the background of combined use of the studied cryoextract and diclofenac sodium, the anti-inflammatory activity was 46.4 %. This suggests a suppressive effect on the production of prostaglandins as a possible mechanism of anti-exudative action of cryopreserved placenta extract.