Modulation of Maize Rhizosphere Microbiota Composition by Inoculation with Azospirillum argentinense Az39 (Formerly A. brasilense Az39)

Coniglio, Anahí; Larama, Giovanni; Molina, R.; Mora, Verónica; Torres, Daniela; Marin, Anelis Maria; Avila, Andrés Ignacio; NoirCarlan, Coline Lede; Erijman, Leonardo; Figuerola, Eva Lucia Margarita; Souza, Emanuel Maltempi de; Cassán, Fabricio

doi:10.1007/s42729-022-00909-6

Cited by 12 publications

(5 citation statements)

References 80 publications

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“…Similarly, co-inoculation with Rhizobium pisi and Pseudomonas monteilii has been an effective biofertilization strategy for common bean production in Cuban soils [23]. Other studies have also shown synergism between rhizobia and PGPRs to increase the growth and production of maize and beans under different environmental conditions [24][25][26][27]. An increase in the abundance of nitrogen-fixing genera in the maize endophytic microbiome affected by inoculation with PGPR UM270 was not clearly detected in the rhizospheric microbiome, perhaps because of the capacity (and preference) of these rhizobia to colonize legume rhizospheres (such as Phaseolus vulgaris).…”

Section: Discussionmentioning

confidence: 99%

Diversity of the Maize Root Endosphere and Rhizosphere Microbiomes Modulated by the Inoculation with Pseudomonas fluorescens UM270 in a Milpa System

Rojas-Sánchez,

Castelán-Sánchez,

Garfias-Zamora

et al. 2024

Plants

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Milpa is an agroecological production system based on the polyculture of plant species, with corn featuring as a central component. Traditionally, the milpa system does not require the application of chemicals, and so pest attacks and poor growth in poor soils can have adverse effects on its production. Therefore, the application of bioinoculants could be a strategy for improving crop growth and health; however, the effect of external inoculant agents on the endemic microbiota associated with corn has not been extensively studied. Here, the objective of this work was to fertilize a maize crop under a milpa agrosystem with the PGPR Pseudomonas fluorescens UM270, evaluating its impact on the diversity of the rhizosphere (rhizobiome) and root endophytic (root endobiome) microbiomes of maize plants. The endobiome of maize roots was evaluated by 16S rRNA and internal transcribed spacer region (ITS) sequencing, and the rhizobiome was assessed by metagenomic sequencing upon inoculation with the strain UM270. The results showed that UM270 inoculation of the rhizosphere of P. fluorescens UM270 did not increase alpha diversity in either the monoculture or milpa, but it did alter the endophytic microbiome of maize plant roots by stimulating the presence of bacterial operational taxonomic units (OTUs) of the genera Burkholderia and Pseudomonas (in a monoculture), whereas, in the milpa system, the PGPR stimulated greater endophytic diversity and the presence of genera such as Burkholderia, Variovorax, and N-fixing rhizobia genera, including Rhizobium, Mesorhizobium, and Bradyrhizobium. No clear association was found between fungal diversity and the presence of strain UM270, but beneficial fungi, such as Rizophagus irregularis and Exophiala pisciphila, were detected in the Milpa system. In addition, network analysis revealed unique interactions with species such as Stenotrophomonas sp., Burkholderia xenovorans, and Sphingobium yanoikuyae, which could potentially play beneficial roles in the plant. Finally, the UM270 strain does not seem to have a strong impact on the microbial diversity of the rhizosphere, but it does have a strong impact on some functions, such as trehalose synthesis, ammonium assimilation, and polyamine metabolism. The inoculation of UM270 biofertilizer in maize plants modifies the rhizo- and endophytic microbiomes with a high potential for stimulating plant growth and health in agroecological crop models.

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Section: Discussionmentioning

confidence: 99%

Diversity of the Maize Root Endosphere and Rhizosphere Microbiomes Modulated by the Inoculation with Pseudomonas fluorescens UM270 in a Milpa System

Rojas-Sánchez,

Castelán-Sánchez,

Garfias-Zamora

et al. 2024

Plants

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“…The analysis of the maize rhizospheric community of plants inoculated with Azospirillum argentinense strain Az39 indicated that the colonization with the bacterium induced an increase in the relative abundance of beneficial bacterial genera [ 63 ]. Also, inoculation of Azospirillum lipoferum strain CRT1 in maize seeds resulted in a significant change in the composition of the native rhizobacteria community [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…A study performed with these two strains confirmed that they were unable to colonize internally leaves [ 65 ], indicating that they may be very specific for root rhizosphere. The inoculation of A. argentinense strain Az39 also indicated that the genus became the most abundant in the maize rhizosphere [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Bender

Alves²,

Silva³

et al. 2022

IJMS

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Biological nitrogen fixation (BNF) is a key process for the N input in agriculture, with outstanding economic and environmental benefits from the replacement of chemical fertilizers. However, not all symbioses are equally effective in fixing N2, and a major example relies on the high contribution associated with the soybean (Glycine max), contrasting with the low rates reported with the common bean (Phaseolus vulgaris) crop worldwide. Understanding these differences represents a major challenge that can help to design strategies to increase the contribution of BNF, and next-generation sequencing (NGS) analyses of the nodule and root microbiomes may bring new insights to explain differential symbiotic performances. In this study, three treatments evaluated in non-sterile soil conditions were investigated in both legumes: (i) non-inoculated control; (ii) inoculated with host-compatible rhizobia; and (iii) co-inoculated with host-compatible rhizobia and Azospirillum brasilense. In the more efficient and specific symbiosis with soybean, Bradyrhizobium presented a high abundance in nodules, with further increases with inoculation. Contrarily, the abundance of the main Rhizobium symbiont was lower in common bean nodules and did not increase with inoculation, which may explain the often-reported lack of response of this legume to inoculation with elite strains. Co-inoculation with Azospirillum decreased the abundance of the host-compatible rhizobia in nodules, probably because of competitiveness among the species at the rhizosphere, but increased in root microbiomes. The results showed that several other bacteria compose the nodule microbiomes of both legumes, including nitrogen-fixing, growth-promoters, and biocontrol agents, whose contribution to plant growth deserves further investigation. Several genera of bacteria were detected in root microbiomes, and this microbial community might contribute to plant growth through a variety of microbial processes. However, massive inoculation with elite strains should be better investigated, as it may affect the root microbiome, verified by both relative abundance and diversity indices, that might impact the contribution of microbial processes to plant growth.

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“…The authors reported that three of the most abundant genera associated with A. argentinense Az39 inoculation were Burkholderia , Massilia , and Sphingobium . Unfortunately, beneficial interacting networks were not evaluated to potentially detect synergism between the rhizosphere bacteria and the Az39 strain [ 102 ].…”

Section: Plant Microbiome Engineering Strategiesmentioning

confidence: 99%

Rhizobiome Transplantation: A Novel Strategy beyond Single-Strain/Consortium Inoculation for Crop Improvement

Orozco-Mosqueda,

Kumar,

Babalola

et al. 2023

Plants

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The growing human population has a greater demand for food; however, the care and preservation of nature as well as its resources must be considered when fulfilling this demand. An alternative employed in recent decades is the use and application of microbial inoculants, either individually or in consortium. The transplantation of rhizospheric microbiomes (rhizobiome) recently emerged as an additional proposal to protect crops from pathogens. In this review, rhizobiome transplantation was analyzed as an ecological alternative for increasing plant protection and crop production. The differences between single-strain/species inoculation and dual or consortium application were compared. Furthermore, the feasibility of the transplantation of other associated micro-communities, including phyllosphere and endosphere microbiomes, were evaluated. The current and future challenges surrounding rhizobiome transplantation were additionally discussed. In conclusion, rhizobiome transplantation emerges as an attractive alternative that goes beyond single/group inoculation of microbial agents; however, there is still a long way ahead before it can be applied in large-scale agriculture.

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Modulation of Maize Rhizosphere Microbiota Composition by Inoculation with Azospirillum argentinense Az39 (Formerly A. brasilense Az39)

Cited by 12 publications

References 80 publications

Diversity of the Maize Root Endosphere and Rhizosphere Microbiomes Modulated by the Inoculation with Pseudomonas fluorescens UM270 in a Milpa System

Diversity of the Maize Root Endosphere and Rhizosphere Microbiomes Modulated by the Inoculation with Pseudomonas fluorescens UM270 in a Milpa System

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Rhizobiome Transplantation: A Novel Strategy beyond Single-Strain/Consortium Inoculation for Crop Improvement

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