Modulation of luminal L-alanine transport in proximal tubular cells of frog kidney induced by low micromolar Cd2+ concentration

Nesovic-Ostojic, Jelena; Kovačević, Sanjin; Spasić, Svetolik; Lopičić, Srdjan; Todorović, Jovan; Dinčić, Marko; Stanojević, Miroslav; Savin, Marina; Milovanović, Aleksandar; Cemerikic, Dusan

doi:10.1016/j.cbpc.2018.11.007

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…Glycine-alanine complexes were shown with antitumor activity [ 51 ]. Alanine could prevent kidney stones, assist glucose metabolism, help ease hypoglycemia, and improve physical energy [ 52 ]. Furthermore, an increased level of ribose in the probiotic L. plantarum K25 fermented milk might be beneficial since oral administration of ribose was reported to improve athletic performance by boosting muscle energy and making it readily available, and also to improve symptoms of diseases such as chronic fatigue syndrome, fibromyalgia and coronary artery disease [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach

Zhang¹,

Jiang²,

Cai³

et al. 2020

J. Microbiol. Biotechnol.

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Addition of probiotics to yogurt with desired health benefits is gaining increasing attention. To further understand the effect of probiotic Lactobacillus plantarum on the quality and function of fermented milk, probiotic fermented milk (PFM) made with probiotic L. plantarum K25 and yogurt starter (L. delbrueckii ssp. bulgaricus and Streptococcus thermophilus) was compared with the control fermented milk (FM) made with only the yogurt starter. The probiotic strain was shown to survive well with a viable count of 7.1 ± 0.1 log CFU/g in the PFM sample after 21 days of storage at 4 o C. The strain was shown to promote formation of volatiles such as acetoin and 2,3-butanediol with milk fragrance, and it did not cause post-acidification during refrigerated storage. Metabolomics analysis by GC-MS datasets coupled with multivariate statistical analysis showed that addition of L. plantarum K25 increased formation of over 20 metabolites detected in fermented milk, among which γ-aminobutyric acid was the most prominent. Together with several other metabolites with relatively high levels in fermented milk such as glyceric acid, malic acid, succinic acid, glycine, alanine, ribose, and 1,3-dihydroxyacetone, they might play important roles in the probiotic function of L. plantarum K25. Further assay of the bioactivity of the PFM sample showed significant (p < 0.05) increase of ACE inhibitory activity from 22.3% at day 1 to 49.3% at day 21 of the refrigerated storage. Therefore, probiotic L. plantarum K25 could be explored for potential application in functional dairy products.

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Section: Resultsmentioning

confidence: 99%

Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach

Zhang¹,

Jiang²,

Cai³

et al. 2020

J. Microbiol. Biotechnol.

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The effects of cadmium on the transport processes in proximal tubular cells of kidneys

Pejović¹,

Kovačević²,

Ostojic³

2022

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Cadmium (Cd) is an extremely toxic metal that is widespread in nature. Due to its favorable properties, it was widely used in the industry for the production of alkaline batteries, accumulators, pigments, and colored alloys. However, it has been shown that exposure to low concentrations of cadmium leads to damage to many organs and organ systems, and the use of this metal in industry is reduced, and it is replaced by other, less harmful materials. Today, fossil fuel combustion and cigarette consumption are important sources of cadmium exposure. Numerous studies have examined the toxic effects of cadmium and they highlight the kidneys, liver, gonads as the organs that suffer the most damage. The kidneys, as the main place of cadmium storage in the body, are mostly exposed to its toxic effects. In the proximal tubular cells of the kidney, exposure to cadmium disrupts transport processes. Although ionized cadmium (Cd 2+) is thought to be largely responsible for the damage that occurs, the role of the cadmium and metallothionein complex (Cd-MT) cannot be ignored. Peritubular exposure to ionized cadmium indirectly leads to a decrease in the activity of the Na + /L-alanine cotransporter and a decrease in the rate of slow repolarization of the luminal membrane, while the Cd-MT complex leads to both direct and indirect inhibition of this transporter. Also, the Cd-MT complex inhibits Na + /Glucosa cotransporter activity. Exposure to cadmium also leads to a decrease in the endocytic uptake of low molecular weight proteins, which is accompanied by microalbuminuria.

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Modulation of luminal L-alanine transport in proximal tubular cells of frog kidney induced by low micromolar Cd2+ concentration

Cited by 2 publications

References 37 publications

Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach

Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach

The effects of cadmium on the transport processes in proximal tubular cells of kidneys

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