Modulation of K+-channels in p-neurones of the leech CNS by phosphorylation

Goldermann, M.; Hanke, Wolfgang; Schlue, Wolf-R.

doi:10.1007/bf00193789

Cited by 7 publications

(3 citation statements)

References 20 publications

(9 reference statements)

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“…An extensive characterization of Na + , K + , and Ca 2+ currents in the leech CNS has previously been examined using voltage-clamp measurements, ion replacement, and pharmacological blockers to understand the role that these ionic currents play in cultured leech Retzius (R), anterior pagoda (AP) and sensory neurons, attributing different membrane properties to distinct ion channel complements [ 49 ]. In the pressure-sensitive mechanosensory (P) neurons, it was shown that the probabilities of K + -channel open and closed states could be modulated by different phosphorylation events mediated by a 5HTR subtype [ 50 ]. The fast-transient A-type potassium current also increased with the age of cultured AP neurons, while other potassium currents remained unchanged [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

et al. 2018

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The medicinal leech is one of the most venerated model systems for the study of fundamental nervous system principles, ranging from single-cell excitability to complex sensorimotor integration. Yet, molecular analyses have yet to be extensively applied to complement the rich history of electrophysiological study that this animal has received. Here, we generated the first de novo transcriptome assembly from the entire central nervous system of Hirudo verbana, with the goal of providing a molecular resource, as well as to lay the foundation for a comprehensive discovery of genes fundamentally important for neural function. Our assembly generated 107,704 contigs from over 900 million raw reads. Of these 107,704 contigs, 39,047 (36%) were annotated using NCBI’s validated RefSeq sequence database. From this annotated central nervous system transcriptome, we began the process of curating genes related to nervous system function by identifying and characterizing 126 unique ion channel, receptor, transporter, and enzyme contigs. Additionally, we generated sequence counts to estimate the relative abundance of each identified ion channel and receptor contig in the transcriptome through Kallisto mapping. This transcriptome will serve as a valuable community resource for studies investigating the molecular underpinnings of neural function in leech and provide a reference for comparative analyses.

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Section: Discussionmentioning

confidence: 99%

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

et al. 2018

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“…5HT modulates several membrane conductances in leech neurons. For example it modulates Cl Ϫ channels (Ali et al 1998;Lessmann and Dietzel 1995) and K ϩ channels (Goldermann et al 1994) in P sensory neurons. In addition, 5HT modulates voltage-activated K ϩ channels (Acosta-Urquidi et al 1989) and a Cl Ϫ conductance (Munsch and Schlue 1993) in the Retzius cells.…”

Section: Role Of the Na ϩ /K ϩ Atpase In The Ahp Amplitude Enhancementmentioning

confidence: 99%

Activity-Dependent Increase of the AHP Amplitude in T Sensory Neurons of the Leech

Scuri

Mozzachiodi

Brunelli

2002

Journal of Neurophysiology

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We identified a new form of activity-dependent modulation of the afterhyperpolarization (AHP) in tactile (T) sensory neurons of the leech Hirudo medicinalis. Repetitive intracellular stimulation with 30 trains of depolarizing impulses at 15-s inter-stimulus interval (ISI) led to an increase of the AHP amplitude (~60% of the control). The enhancement of AHP lasted for >/=15 min. The AHP increase was also elicited when a T neuron was activated by repetitive stimulation of its receptive field. The ISI was a critical parameter for the induction and maintenance of AHP enhancement. ISI duration had to fit within a time window with the upper limit of 20 s to make the training effective to induce an enhancement of the AHP amplitude. After recovery from potentiation, AHP amplitude could be enhanced once again by delivering another training session. The increase of AHP amplitude persisted in high Mg(2+) saline, suggesting an intrinsic cellular mechanism for its induction. Previous investigations reported that AHP of leech T neurons was mainly due to the activity of the Na(+)/K(+) ATPase and to a Ca(2+)-dependent K(+) current (I(K/Ca)). In addition, it has been demonstrated that serotonin (5HT) reduces AHP amplitude through the inhibition of the Na(+)/K(+) ATPase. By blocking the I(K/Ca) with pharmacological agents, such as cadmium and apamin, we still observed an increase of the AHP amplitude after repetitive stimulation, whereas 5HT application completely inhibited the AHP increment. These data indicate that the Na(+)/K(+) ATPase is involved in the induction and maintenance of the AHP increase after repetitive stimulation. Moreover, the AHP increase was affected by the level of serotonin in the CNS. Finally, the increase of the AHP amplitude produced a lasting depression of the synaptic connection between two T neurons, suggesting that this activity-dependent phenomenon might be involved in short-term plasticity associated with learning processes.

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“…These E m changes are mainly due to the direct activation of 5-HT-gated Cl Ϫ channels (Lessmann and Dietzel, 1995b;Ali et al, 1998) as well as to the activation of unselective cation channels via metabotropic 5-HT receptors (Catarsi and Drapeau, 1997;Drapeau et al, 1989). Beside these well-characterized systems, the existence of other 5-HT-dependent conductances has been proposed (Goldermann et al, 1994;Lessmann and Dietzel, 1991;Walz and Schlue, 1982).…”

Section: Introductionmentioning

confidence: 96%

Ca²⁺ influx into identified leech neurons induced by 5‐hydroxytryptamine

Dierkes

Schlue

2004

J. Neurobiol.

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The role of 5-hydroxytryptamine (5-HT, serotonin) in the control of leech behavior is well established and has been analyzed extensively on the cellular level; however, hitherto little is known about the effect of 5-HT on the cytosolic free calcium concentration ([Ca(2+)](i)) in leech neurons. As [Ca(2+)](i) plays a pivotal role in numerous cellular processes, we investigated the effect of 5-HT on [Ca(2+)](i) (measured by Fura-2) in identified leech neurons under different experimental conditions, such as changed extracellular ion composition and blockade of excitatory synaptic transmission. In pressure (P), lateral nociceptive (N1), and Leydig neurons, 5-HT induced a [Ca(2+)](i) increase which was predominantly due to Ca(2+) influx since it was abolished in Ca(2+)-free solution. The 5-HT-induced Ca(2+) influx occurred only if the cells depolarized sufficiently, indicating that it was mediated by voltage-dependent Ca(2+) channels. In P and N1 neurons, the membrane depolarization was due to Na(+) influx through cation channels coupled to 5-HT receptors, whereby the dose-dependency suggests an involvement in excitatory synaptic transmission. In Leydig neurons, 5-HT receptor-coupled cation channels seem to be absent. In these cells, the membrane depolarization activating the voltage-dependent Ca(2+) channels was evoked by 5-HT-triggered excitatory glutamatergic input. In Retzius, anterior pagoda (AP), annulus erector (AE), and median nociceptive (N2) neurons, 5-HT had no effect on [Ca(2+)](i).

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Modulation of K+-channels in p-neurones of the leech CNS by phosphorylation

Cited by 7 publications

References 20 publications

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

Activity-Dependent Increase of the AHP Amplitude in T Sensory Neurons of the Leech

Ca²⁺ influx into identified leech neurons induced by 5‐hydroxytryptamine

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Modulation of K+-channels in p-neurones of the leech CNS by phosphorylation

Cited by 7 publications

References 20 publications

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

Activity-Dependent Increase of the AHP Amplitude in T Sensory Neurons of the Leech

Ca2+ influx into identified leech neurons induced by 5‐hydroxytryptamine

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Ca²⁺ influx into identified leech neurons induced by 5‐hydroxytryptamine