Modulation of Intestinal Phosphate Transport in Young Goats Fed a Low Phosphorus Diet

Behrens, Joie L.; Schnepel, Nadine; Hansen, Kirstin Anderson; Hustedt, Karin; Burmester, Marion; Klinger, Stefanie; Breves, Gerhard; Muscher-Banse, Alexandra S.

doi:10.3390/ijms22020866

Cited by 3 publications

(3 citation statements)

References 89 publications

(128 reference statements)

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“…In this study, the highest expression levels of intestinal P transport-related genes were found in spotted seabass fed the diet with an available P level of 0.35%. P deficiency has also been found, in other studies, to cause the upregulation of genes related to P transport [46]. Combining the results of the ADC of P and whole-body P deposition efficiency, it is hypothesized that this can be a regulatory mechanism for the perch to adapt to the P-deficient environment by improving the absorption, utilization and deposition of P.…”

Section: Discussionmentioning

confidence: 60%

Physiological Response of Spotted Seabass (Lateolabrax maculatus) to Different Dietary Available Phosphorus Levels and Water Temperature: Changes in Growth, Lipid Metabolism, Antioxidant Status and Intestinal Microbiota

Guo,

Wang,

Song

et al. 2023

Antioxidants

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A 10-week growth experiment was conducted to assess the physiological response of spotted seabass (Lateolabrax maculatus) raised at moderate (27 °C) and high temperatures (33 °C) to different dietary available phosphorus (P) levels. Five diets with available P levels of 0.35, 0.55, 0.71, 0.82 and 0.92% were formulated, respectively. A water temperature of 33 °C significantly decreased growth performance and feed utilization, and increased oxidative stress and lipid deposition of spotted seabass compared with 27 °C. A second-order polynomial regression analysis based on weight gain (WG) showed that the available P requirement of spotted seabass raised at 27 °C and 33 °C was 0.72% and 0.78%, respectively. The addition of 0.71–0.82% P to the diet improved the growth performance, feed utilization, and antioxidant capacity of spotted seabass and alleviated the excessive lipid deposition compared with the low-P diet (0.35% P). Moreover, the addition of 0.71–0.92% P to diets increased the diversity of intestinal microbiota and the relative abundance of Lactococcus lactis and decreased the relative abundance of Plesiomonas compared with the low-P diet. Thus, dietary supplementation with 0.71–0.82% P improved the growth performance, antioxidant capacity and microbial composition of spotted seabass, and alleviated the disturbance of lipid metabolism caused by high temperature or low-P diet.

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Section: Discussionmentioning

confidence: 60%

Physiological Response of Spotted Seabass (Lateolabrax maculatus) to Different Dietary Available Phosphorus Levels and Water Temperature: Changes in Growth, Lipid Metabolism, Antioxidant Status and Intestinal Microbiota

Guo,

Wang,

Song

et al. 2023

Antioxidants

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“…N-reduced feeding had no effect on these parameters. The data on P-reduction were partially published by Behrens et al [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Modulation of GCN2/eIF2α/ATF4 Pathway in the Liver and Induction of FGF21 in Young Goats Fed a Protein- and/or Phosphorus-Reduced Diet

Weber¹,

Hustedt²,

Schnepel³

et al. 2023

IJMS

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Mammals respond to amino acid (AA) deficiency by initiating an AA response pathway (AAR) that involves the activation of general control nonderepressible 2 (GCN2), phosphorylation of eukaryotic translation initiation factor 2α (eIF2α), and activation of transcription factor 4 (ATF4). In this study, the effects of protein (N) and/or phosphorus (P) restriction on the GCN2/eIF2α/ATF4 pathway in the liver and the induction of fibroblast growth factor 21 (FGF21) in young goats were investigated. An N-reduced diet resulted in a decrease in circulating essential AA (EAA) and an increase in non-essential AA (NEAA), as well as an increase in hepatic mRNA expression of GCN2 and ATF4 and protein expression of GCN2. Dietary N restriction robustly increased both hepatic FGF21 mRNA expression and circulating FGF21 levels. Accordingly, numerous significant correlations demonstrated the effects of the AA profile on the AAR pathway and confirmed an association. Furthermore, activation of the AAR pathway depended on the sufficient availability of P. When dietary P was restricted, the GCN2/eIF2α/ATF4 pathway was not initiated, and no increase in FGF21 was observed. These results illustrate how the AAR pathway responds to N- and/or P-reduced diets in ruminants, thus demonstrating the complexity of dietary component changes.

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“…In a study by Muscher and Huber (4), N-reduction also resulted in alterations in vitamin D metabolism. P-reduction also resulted in modulation of mineral homeostasis in goats and sheep with reduced plasma concentration of P i and concomitant high plasma Ca levels in the blood (6,7). The homeostasis of P i and Ca in the body is tightly regulated by various hormones, primarily controlled by the interaction of active vitamin D (1,25(OH) 2 D 3 , calcitriol), parathyroid hormone (PTH), and fibroblast growth factor 23 (FGF23) (8).…”

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confidence: 99%

Effects of dietary nitrogen and/or phosphorus reduction on mineral homeostasis and regulatory mechanisms in young goats

Zillinger,

Hustedt,

Schnepel

et al. 2024

Front. Vet. Sci.

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IntroductionThe reduction of nitrogen (N) and phosphorus (P) in ruminant feed is desirable due to costs and negative environmental impact. Ruminants are able to utilize N and P through endogenous recycling, particularly in times of scarcity. When N and/or P were reduced, changes in mineral homeostasis associated with modulation of renal calcitriol metabolism occurred. The aim of this study was to investigate the potential effects of dietary N- and/or P-reduction on the regulatory mechanisms of mineral transport in the kidney and its hormonal regulation in young goats.ResultsDuring N-reduction, calcium (Ca) and magnesium (Mg) concentrations in blood decreased, accompanied by a lower protein expression of cytochrome P450 family 27 subfamily B member 1 (CYP27B1) (p = 0.016). The P-reduced fed goats had low blood phosphate concentrations with simultaneously high Ca and Mg levels. The insulin-like growth factor 1 concentrations decreased significantly with P-reduction. Furthermore, gene expression of CYP27B1 (p < 0.001) and both gene (p = 0.025) and protein (p = 0.016) expression of the fibroblast growth factor receptor 1c isoform in the kidney were also significantly reduced during a P-reduced diet. ERK1/2 activation exhibited a trend toward reduction in P-reduced animals. Interestingly, calcitriol concentrations remained unaffected by either restriction individually, but interacted significantly with N and P (p = 0.014). Additionally, fibroblast growth factor 23 mRNA expression in bone decreased significantly with P-restriction (p < 0.001).DiscussionThese results shed light on the complex metabolic and regulatory responses of mineral transport of young goats to dietary N and P restriction.

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Modulation of Intestinal Phosphate Transport in Young Goats Fed a Low Phosphorus Diet

Cited by 3 publications

References 89 publications

Physiological Response of Spotted Seabass (Lateolabrax maculatus) to Different Dietary Available Phosphorus Levels and Water Temperature: Changes in Growth, Lipid Metabolism, Antioxidant Status and Intestinal Microbiota

Physiological Response of Spotted Seabass (Lateolabrax maculatus) to Different Dietary Available Phosphorus Levels and Water Temperature: Changes in Growth, Lipid Metabolism, Antioxidant Status and Intestinal Microbiota

Modulation of GCN2/eIF2α/ATF4 Pathway in the Liver and Induction of FGF21 in Young Goats Fed a Protein- and/or Phosphorus-Reduced Diet

Effects of dietary nitrogen and/or phosphorus reduction on mineral homeostasis and regulatory mechanisms in young goats

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