Modulation of inducible nitric oxide synthase pathway by eugenol and telmisartan in carbon tetrachloride-induced liver injury in rats

Fathy, Moustafa; Khalifa, Esraa M.M.A.; Fawzy, Michael Atef

doi:10.1016/j.lfs.2018.11.031

Cited by 52 publications

(33 citation statements)

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“…It was reported that EUG exerted various pharmacological activities [20], and showed a hepatoprotective effect by improving the hepatic antioxidant capacity and declining the inflammation via the downregulation of the iNOS pathway [14]. Therefore, we hypothesized that treatment by AT-MSCs with EUG would yield an utmost antifibrotic effect and improvement in hepatic function compared with treatment with AT-MSCs alone.…”

Section: Discussionmentioning

confidence: 96%

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AT-MSCs Antifibrotic Activity is Improved by Eugenol through Modulation of TGF-β/Smad Signaling Pathway in Rats

et al. 2020

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For hepatic failure, stem cell transplantation has been chosen as an alternative therapy, especially for mesenchymal stem cells (MSCs). The aim of this study was to investigate the effect of eugenol (EUG) on the in vivo antifibrotic activity of adipose tissue-derived MSCs (AT-MSCs) and the underlying mechanism. After characterization of MSCs, rats were divided into five groups, Group 1 (normal control), Group 2 (CCl4), Group 3 (CCl4 + AT-MSCs), Group 4 (CCl4 + EUG) and Group 5 (CCl4 + AT-MSCs + EUG). Biochemical and histopathological investigations were performed. Furthermore, expression of type 1 collagen, α-SMA, TGF-β1, Smad3 and P-Smad3 was estimated. Compared to the single treatment with AT-MSCs, the combination treatment of the fibrotic rats with AT-MSCs and EUG significantly improved the plasma fibrinogen concentration, IL-10 level and proliferating cell nuclear antigen expression, and also significantly decreased the serum levels of liver enzymes, IL-6, IL-1β, TNF-α, type III collagen, hyaluronic acid, hydroxyproline and the TGF-β growth factor. Furthermore, the combination treatment significantly decreased the hepatic expression of fibrotic markers genes (Type 1 collagen and α-SMA) and proteins (α-SMA, TGF-β1 and phospho-Smad3) more than the treatment with AT-MSCs alone. We demonstrated that the combination treatment with EUG and AT-MSCs strongly inhibited the advancement of CCl4-induced hepatic fibrosis, compared with AT-MSCs alone, through TGF-β/Smad pathway inhibition. This approach is completely novel, so more investigations are necessary to improve our perception of the underlying molecular mechanisms accountable for the effects of EUG on the antifibrotic potential of AT-MSCs.

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Section: Discussionmentioning

confidence: 96%

“…Eugenol diluted in olive oil 1:1 (v/v) was intraperitonealy (i.p.) injected daily at a dose 10 mg/kg [14].…”

Section: Animals and The Experimental Designmentioning

confidence: 99%

AT-MSCs Antifibrotic Activity is Improved by Eugenol through Modulation of TGF-β/Smad Signaling Pathway in Rats

et al. 2020

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“…A significant decrease in nitrite levels was observed in cells stimulated with T. cruzi and treated with essential oil or eugenol. Eugenol notably modulates NO production, inducing suppression of NO production and iNOS protein expression in carbon tetrachloride-induced liver injury in rats [54] and in nicotineinduced murine peritoneal macrophages [55]. Eugenolol and glyceryl-isoeugenol, two derivatives of eugenol, suppressed LPS-induced iNOS expression by downregulating NF-kB and AP-1 through the inhibition of the MAPKs and Akt/IkB-alpha signaling pathways [56].…”

Section: Discussionmentioning

confidence: 99%

GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol

Teles

Silva-Silva

Fernandes

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC50 of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC50 of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC50 of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; p = 0.002) and eugenol (48.05%; p = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis.

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“…The overwhelming oxidant stress observed following codeine treatment ultimately excites an inflammatory reaction evident by a rise in testicular NO. It may be secondary to stimulation of NF-kB and enhancement of inducible nitric oxide synthase (iNOS) [49,50], essential factors in NO production. NO couples with O 2to form peroxynitrite (ONOO 2 -) which is hugely more reactive and harmful as compared to NO or O 2alone.…”

Section: Discussionmentioning

confidence: 99%

Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO and caspase 3

Akhigbe

Ajayi

2019

Preprint

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Background: Codeine, a 3-methylmorphine, and other related opioids have been implicated in androgen suppression, although the associated mechanisms remain unclear.Aim: Therefore, the objective of the current study was to elucidate the in vivo molecular mechanisms underlying codeine-induced androgen suppression.Methods: This study made use of Twenty-one healthy male rabbits, distributed into three groups randomly, control and codeine-treated groups. The control had 1ml of normal saline daily p.o.The codeine-treated groups received either 4mg/kg b.w of codeine or 10mg/kg b.w of codeine p.o. for six weeks. Reproductive hormonal profile, testicular weight, enzymes, oxidative and inflammatory parameters, histological examination and apoptosis marker were evaluated to examine the effects of codeine use.Key findings: Oral administration of codeine resulted in testicular atrophy and alterations in testicular histomorphology, elevated testicular enzymes, and suppression of circulatory and intratesticular testosterone. These changes were associated with a marked rise in oxidative markers, including oxidative DNA damage, inflammatory response, and caspase-dependent apoptosis.Significance: In conclusion, chronic codeine use resulted in testicular degeneration and testosterone suppression, which may be attributable to nitric oxide-/oxidativestress-mediated caspase-dependent apoptotic testicular cell death.

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Modulation of inducible nitric oxide synthase pathway by eugenol and telmisartan in carbon tetrachloride-induced liver injury in rats

Cited by 52 publications

References 53 publications

AT-MSCs Antifibrotic Activity is Improved by Eugenol through Modulation of TGF-β/Smad Signaling Pathway in Rats

AT-MSCs Antifibrotic Activity is Improved by Eugenol through Modulation of TGF-β/Smad Signaling Pathway in Rats

GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol

Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO and caspase 3

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