Modulation ofIdeonella sakaiensisPETase active site flexibility and activity on morphologically distinct substrates by surface charge engineering
Ke Ding,
Zarina Levitskaya,
Barindra Sana
et al.
Abstract:Enzymatic hydrolysis of polyethylene terephthalate (PET) waste is a compelling strategy for environmentally friendly recycling of a major pollutant. Here, we investigate the effects of surface charge point mutations both proximal and distal to the active site of the mesophilic PET-degrading enzyme from Ideonella sakaienses (IsPETase) and an engineered thermostable variant with superior activity, STAR PETase. The vicinal K95A mutation significantly inhibited IsPETase activity on mechanically processed PET powde… Show more
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