Modulation of Host Immunity and Development by Ustilago maydis

Djamei, Armin; Depotter, Jasper R L; Saridis, Georgios; Prokchorchik, Maxim; Barghahn, Sina; Silva, Natalia De Sousa Teixeira E.; Zuo, Weidong; Misas-Villamil, Johana C.; Doehlemann, Gunther

doi:10.1007/978-3-031-16503-0_1

Cited by 3 publications

(2 citation statements)

References 154 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of our knowledge about smut fungi has been derived from a few model smut pathogens, of which the best studied is Ustilago maydis (DC. ), which causes gall formation on all aerial parts of its host plant Zea mays (L.) and its wild relatives Teosinte [3,4]. It shares with all smut fungi a parasitic dikaryotic phase and a saprophytic haploid phase during its life cycle.…”

Section: Smut Fungi Their Development and Disease Symptomsmentioning

confidence: 99%

Manipulation of Auxin Signaling by Smut Fungi during Plant Colonization

Nagarajan,

Khan,

Djamei

2023

JoF

View full text Add to dashboard Cite

A common feature of many plant-colonizing organisms is the exploitation of plant signaling and developmental pathways to successfully establish and proliferate in their hosts. Auxins are central plant growth hormones, and their signaling is heavily interlinked with plant development and immunity responses. Smuts, as one of the largest groups in basidiomycetes, are biotrophic specialists that successfully manipulate their host plants and cause fascinating phenotypes in so far largely enigmatic ways. This review gives an overview of the growing understanding of how and why smut fungi target the central and conserved auxin growth signaling pathways in plants.

show abstract

Section: Smut Fungi Their Development and Disease Symptomsmentioning

confidence: 99%

Manipulation of Auxin Signaling by Smut Fungi during Plant Colonization

Nagarajan,

Khan,

Djamei

2023

JoF

View full text Add to dashboard Cite

show abstract

“…One of the notable advantages of working with U. maydis yeast is its amenability to genetic manipulation. This feature enables the creation of mutants, facilitating research into phytopathogenic processes and their interactions with host plants [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Ustilago maydis Yeast Mutant Produces Cytosolic Melanin by Tyrosine-Tyrosinase Activity with Stain Biosorption Capability

Martínez-López,

Vázquez-Carrada,

Flores-Herrera

et al. 2023

Applied Sciences

View full text Add to dashboard Cite

Ustilago maydis is a biotrophic basidiomycete fungus that infects corn plants and works as an excellent phytopathogen model, facilitating numerous genetic transformations for studying the mechanisms of plant infection. A random mutation event in the mutant strains designed to investigate the physiological significance of two plasma membrane proton-ATPases in this model resulted in a pigmented phenotype strain. For this study, the FB2 strain and the ΔPMA1 mutant were chosen to assess the pigment, which was confirmed as melanin through thin-layer chromatography, UV, and IR spectrophotometry. The melanin was observed to accumulate in the cytosol, as evident from scanning and transmission electron microscopy, and did not interfere with normal cell growth in yeast extract peptone dextrose media or minimal media. Notably, the mutant exhibited a 25% higher melanin yield compared to wild-type cells. To analyze the melanin synthesis, the tyrosinase activity was measured in a phosphate buffer at pH 6.5. The enzyme demonstrated greater activity with tyrosine as a substrate than with L-3,4 dihydroxyphenylalanine, maintaining the same trend in ion preference. Both FB2 and ΔPMA1 mutant cells were subjected to biosorption experiments, revealing that the mutants with an excess of cytosolic melanin were capable of removing at least 50 ppm of methylene blue. In conclusion, U. maydis can accumulate melanin in the cytosol without adverse physiological effects and this presents biotechnological potential for dye removal.

show abstract

TOPLESS Corepressors as an Emerging Hub of Plant Pathogen Effectors

Khan,

Djamei

2024

MPMI

View full text Add to dashboard Cite

Transcriptional corepressors form an ancient and essential layer of gene expression control in eukaryotes. TOPLESS and TOPLESS-RELATED (TPL)/TPR) proteins constitute a conserved family of Groucho (Gro) / Thymidine uptake 1 (Tup1) type transcriptional corepressors and control diverse growth, developmental, and stress signalling responses in plants. Due to their central and versatile regulatory roles, they act as a signalling hub to integrate various input signalling pathways in the transcriptional responses. Recently, increasing pieces of evidence indicate the roles of TPL/TPR family proteins in the modulation of plant immunity. This is supported by studies on effectors of distantly related pathogens that target TPL/TPR proteins in planta. In this short review, we will summarize the latest findings where pathogens target plant TPL/TPR proteins to manipulate plant signalling responses for the successful invasion of their hosts.

show abstract

Modulation of Host Immunity and Development by Ustilago maydis

Cited by 3 publications

References 154 publications

Manipulation of Auxin Signaling by Smut Fungi during Plant Colonization

Manipulation of Auxin Signaling by Smut Fungi during Plant Colonization

Ustilago maydis Yeast Mutant Produces Cytosolic Melanin by Tyrosine-Tyrosinase Activity with Stain Biosorption Capability

TOPLESS Corepressors as an Emerging Hub of Plant Pathogen Effectors

Contact Info

Product

Resources

About