Modulation of hepatic glutathione transferases isoenzymes in three bivalve species exposed to purified microcystin-LR and Microcystis extracts

Carneiro, Mariana; Paulo, Antas; Reis, Bruno; Azevedo, Joana; Osório, Hugo; Campos, Alexandre; Vasconcelos, Vı́tor; Martins, José

doi:10.1016/j.toxicon.2017.07.003

Cited by 10 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also concerning enzymatic activity and protein expression, significant alterations in GST according to bivalve species, were reported. It was denoted in this study a slow response of mussel's GST system, comparatively to the GST systems from clams R. philippinarum and C. fluminea [23]. No variations in GST system or other protein markers referred by other authors were observed in the present study.…”

Section: Metabolic Responses Of Mussels To Toxic Cyanobacteriasupporting

confidence: 44%

“…Regarding estuarine and marine bivalve species, a question still remains whether these exhibit the same behavior and tolerance as their freshwater relatives, to feeding on toxic cyanobacteria and exposure to cyanotoxins. Studies have shown that estuarine and marine bivalve species such as Mytilus galloprovincialis and Ruditapes philippinarum modulate their detoxification and oxidative-stress defense systems after exposure to MCs [23,24]. Indeed, contamination of estuarine and coastal areas derived from the flow of contaminated freshwaters from inland to the sea have been reported in different geographical regions [25][26][27], constituting a potential thread to marine ecosystems.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

Toxins

View full text Add to dashboard Cite

Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

show abstract

Section: Metabolic Responses Of Mussels To Toxic Cyanobacteriasupporting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…Decreased activity and transcription of SOD implied weaker antioxidant capacity and immunity of L. vannamei (Pang et al, 2019). GSH was an another effective antioxidant and reported to play a vital role against cyanobacterial toxicity (Carneiro et al, 2017). It was reported that exposure to MC or cyanobacteria increased the GSH content in the liver of both mammals and aquatic animals (Gehringer et al, 2004;BLÁHA et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Toxicity of The Microcystin-Producing Cyanobacterium Microcystis Aeruginosa To Litopenaeus Vannamei

et al. 2022

Preprint

View full text Add to dashboard Cite

Microcystis aeruginosa is a common kind of harmful bloom algae, which was also frequently found as a dominant microalgae specie in shrimp breeding ponds. And it was found that blooms always induced massive death of shrimp, but the toxic effects of M. aeruginosa on Litopenaeus vannamei are still not completely understood. In this paper, the toxicity of M. aeruginosa cells to L. vannamei was examined, and the toxic components in the cells were analyzed through high-pressure liquid chromatography (HLPC). In addition, the immune response of shrimp to the microalgal extract was assessed by measuring the activity of immune-related enzymes, as well as the transcription of the relevant genes. Overall, both M. aeruginosa cells and the algal extract resulted in a 100% mortality rate in shrimp, whereas the cell-free culture medium was ineffective. And HPLC analysis results revealed the presence of microcystin-LR (MC-LR) at a concentration of 190.40 mg/kg of cells. In addition, the activity and gene transcription of two immune related enzymes, SOD and LZM, were both significantly reduced in shrimp hepatopancreas (p<0.05) after injection with cell extract. However, reduced glutathione (GSH) content was slightly increased, but the ratio of GSH to GSSG was down, and the transcription of gst gene function as detoxification, was significantly downregulated (p<0.05). The results demonstrated that M. aeruginosa cell extract was highly toxic to L. vannamei, and exerted a negative effect on shrimp immunity including reduction of antioxidant capacity, antibacterial activity and detoxification activity, due to microcystin-LR.

show abstract

“…A target proteomics approach, combining affinity chromatography and protein purification, 2DE and MALDI-TOF mass spectrometry, was used to investigate the role of glutathione s-transferases (GSTs) in the response of bivalves [37,38]. The studies carried out revealed protein profiles constituted by multiple GST isoforms in bivalve species.…”

Section: Proteomic Studiesmentioning

confidence: 99%

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

et al. 2021

View full text Add to dashboard Cite

Cyanobacteria have long been associated with harmful effects on humans, animals and aquatic biota. Cyanotoxins are their most toxic metabolite. This review summarizes the current research, impacts and future needs in cyanobacterial studies undertaken in Portugal, the southernmost country of Europe, and with a recent multiplication of cyanotoxicity due to climate change events. Microcystins are still the most prevalent, studied and the only regulated cyanotoxins in Portuguese freshwater systems much like most European countries. With the development of some tools, particularly in molecular studies, the recent discovery of cylindrospermopsins, anatoxins and saxitoxins, both genes and toxins, in North and Center ecosystems of our country highlight current impacts that overall communities are facing with increased risks of exposure and uptake to cyanotoxins. Research needs encompass the expansion of studies at all aspects due to the uprising of these cyanotoxins and reinforces the urgent need of increasing the frequency of surveillance to achieve tangible effects of cyanotoxins in Portugal to ultimately implement regulations on cylindrospermopsins, anatoxins and saxitoxins worldwide.

show abstract

Modulation of hepatic glutathione transferases isoenzymes in three bivalve species exposed to purified microcystin-LR and Microcystis extracts

Cited by 10 publications

References 29 publications

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Toxicity of The Microcystin-Producing Cyanobacterium Microcystis Aeruginosa To Litopenaeus Vannamei

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

Contact Info

Product

Resources

About