Modulation of heat shock proteins during macrophage differentiation

Fagone, Paolo; Rosa, Michelino Di; Palumbo, Maria Concetta; Gregorio, Corinne De; Nicoletti, Ferdinando; Malaguarnera, Mariano

doi:10.1007/s00011-012-0506-y

Cited by 28 publications

(20 citation statements)

References 20 publications

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“…However, their quality (protein or mRNA cargo) and not necessarily quantity governs their physiological impact on other cell types. Another secretory product found in ovarian cancer cells was HSP27 but its release pattern does not correlate to any of the findings in THP-1 monocytes, although it is crucial in macrophage polarization [64]. However, we do not propose finding fully differentiated macrophages in our study but rather propose a principle immunomodulatory function of ovarian cancer cell secretory products in monocytes that can be targeted with plasma treatment.…”

Section: Discussioncontrasting

confidence: 41%

Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes

Bekeschus

Wulf

Freund

et al. 2018

Plasma

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Cancers modulate their microenvironment to favor their growth. In particular, monocytes and macrophages are targeted by immuno-modulatory molecules installed by adjacent tumor cells such as ovarian carcinomas. Cold physical plasma has recently gained attention as innovative tumor therapy. We confirmed this for the OVCAR-3 and SKOV-3 ovarian cancer cell lines in a caspase 3/7 independent and dependent manner, respectively. To elaborate whether plasma exposure interferes with their immunomodulatory properties, supernatants of control and plasma-treated tumor cells were added to human THP-1 monocyte cultures. In the latter, modest effects on intracellular oxidation or short-term metabolic activity were observed. By contrast, supernatants of plasma-treated cancer cells abrogated significant changes in morphological and phenotypic features of THP-1 cells compared to those cultured with supernatants of non-treated tumor cell counterparts. This included cell motility and morphology, and modulated expression patterns of nine cell surface markers known to be involved in monocyte activation. This was particularly pronounced in SKOV-3 cells. Further analysis of tumor cell supernatants indicated roles of small particles and interleukin 8 and 18, with MCP1 presumably driving activation in monocytes. Altogether, our results suggest plasma treatment to alleviate immunomodulatory secretory products of ovarian cancer cells is important for driving a distinct myeloid cell phenotype.

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Section: Discussioncontrasting

confidence: 41%

Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes

Bekeschus

Wulf

Freund

et al. 2018

Plasma

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“…Macrophage polarization was obtained as previously described [32]. In brief, the culture medium was removed and cells were cultured for an additional 18 h in RPMI 1640 supplemented with 5 % FBS and LPS (100 ng/ml) plus IFN-γ (100 U/ml) (for M1 polarization) or IL-4 (20 ng/ml) (for M2 polarization) (PeproTech, Milan, Italy).…”

Section: Macrophage Differentiationmentioning

confidence: 99%

Evaluation of CHI3L-1 and CHIT-1 Expression in Differentiated and Polarized Macrophages

et al. 2012

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Chitinase 3-like protein 1 (CHI3L-1) and chitotriosidase (CHIT-1) are members of the chitinase family. CHI3L-1 is a newly recognized protein that is secreted by activated macrophages and neutrophils and expressed in a broad spectrum of inflammatory conditions and cancers. In human plasma, CHIT-1 activity has been proposed as a biochemical marker of macrophage activation. Although CHI3L-1 expression in inflammation is under examination, little is known regarding its regulation during macrophages' full maturation and polarization. In this study, we compared CHI3L-1 and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR. We found that during the maturation of monocytes into macrophages, the expression of both CHI3L-1 and CHIT-1 increased exponentially over time. Additionally, we observed a different regulation of CHI3L-1 and CHIT-1 in undifferentiated monocytes under stimulation with lipopolysaccharide, interferon-γ, and interleukin-4, at the same concentration used to polarize macrophages. Our finding suggests that in the immune response, the role of CHI3L-1 and CHIT-1 is not restricted to innate immunity, but they are also protagonists in acquired immunity.

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“…Macrophage polarization was obtained as previously described [30]. In brief, the culture medium was removed and cells were cultured for an additional 18 h in RPMI 1640 supplemented with 5% FBS and LPS (50 ng/ml) plus IFN-γ (100 U/ml) (for M 1 polarization) or IL-4 (20 ng/ml) (for M 2 polarization) (Peproteck, Milan, Italy).…”

Section: Macrophage Differentiationmentioning

confidence: 99%

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Rosa¹,

Tibullo²,

Malaguarnera³

et al. 2013

MRI

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The chitinase-like proteins YKL-39 (chitinase 3-like-2) and Chitortriosidase (CHIT-1) are members of the chitinases family. YKL-39 expression has been associated with osteoarthritis, whereas CHIT-1 activity is regarded as a biochemical marker of macrophage activation. So far, the physiological or pathological role of YKL-39 in the inflammation is still poorly understood. We compared YKL-39 and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR from mRNA of human monocytes obtained from buffy coat of healthy volunteers, from mRNA of polarized macrophages to classically activated macrophages (or M 1 ), obtained by interferon-γ and lipopolysaccharide exposure, and from mRNA of alternatively activated macrophages (or M 2 ) obtained by interleukin-4 exposure. We demonstrated different variations of YKL-39 and CHIT-1 production during macrophages polarization. CHIT-1 levels gradually increase in the course of the time with a peak of expression between the fifth and the seventh day of culture. In contrast, YKL-39 expression was unaltered in the diverse stage of HMMs differentiation, but increased significantly in M 1 polarized macrophages and reverted to base levels in M 2 polarized macrophages. These findings indicated that the function of YKL-39 is much more restricted and selective than that exerted by CHIT-1.

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Modulation of heat shock proteins during macrophage differentiation

Abstract: Our study revealed that monocytes undergoing maturation differentially regulate the expression of several members of HSPs and that distinct patterns of HSP expression characterize the M1 and M2 effector stages of macrophage life.

Cited by 28 publications

References 20 publications

Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes

Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes

Evaluation of CHI3L-1 and CHIT-1 Expression in Differentiated and Polarized Macrophages

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

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