Modulation of Haemonchus contortus infection by depletion of γδ+ T cells in parasite resistant Canaria Hair Breed sheep

Hernández, Julia N.; Meeusen, Elza Nicole Theresia; Stear, M. J.; Rodrı́guez, Francisco; Piedrafita, David; González, J.

doi:10.1016/j.vetpar.2017.02.021

Cited by 14 publications

(9 citation statements)

References 28 publications

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“…The CHB sheep used in this study have been shown to have enhanced resistance to H contortus after a single infection, with resistance directed against the adult parasite stage manifested in reduced worm length and fecundity . Further studies have shown that this resistance correlates significantly with both δ–T cells and eosinophils and that depletion of δ–T cells abrogates protection as well as the eosinophil correlation . The present study shows a similar effect on adult worms after targeting eosinophilia through recombinant IL‐5 immunization and supports the initial hypothesis that both δ–T cells and eosinophils, possibly in cooperation, are responsible for the effect on adult worms in the resistant CHB sheep.…”

Section: Discussionsupporting

confidence: 82%

“…In the present study, vaccination with recombinant IL-5 resulted Further studies have shown that this resistance correlates significantly with both δ-T cells and eosinophils 8 and that depletion of δ-T cells abrogates protection as well as the eosinophil correlation. 15 The present study shows a similar effect on adult worms after targeting eosinophilia through recombinant IL-5 immunization and supports the initial hypothesis that both δ-T cells and eosinophils, possibly in cooperation, are responsible for the effect on adult worms in the resistant CHB sheep. The exact mechanism of this immunity is still not known; however, it has been shown that gastrointestinal δ-T cells of sheep express IL-5 17 and that IL-5 activates eosinophils 18 and enhances the in vitro killing of H contortus larvae by eosinophils.…”

Section: Several In Vitro Killing or In Vivo Histological And Correlasupporting

confidence: 88%

“…For some components, it has been possible to determine more directly their functional in vivo role by neutralizing activity through monoclonal antibody infusion. For example, the key role of CD4+ and δT cells in protection of different sheep breeds. However, because of the animal size, these are generally laborious and expensive experiments, and specific reagents are not always available for livestock.…”

Section: Discussionmentioning

confidence: 99%

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Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Hernández

Meeusen

Rodrı́guez

et al. 2019

Parasite Immunology

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Eosinophils are prominent effector cells in immune responses against gastrointestinal nematode infections in ruminants, but their in vivo role has been hard to establish in large animals. Interleukin‐5 is a key cytokine in the induction and stimulation of anti‐parasitic eosinophil responses. This study attempted to modulate the eosinophil response in sheep through vaccination with recombinant interleukin‐5 (rIL‐5) and determine the effect on subsequent Haemonchus contortus infection. Nematode‐resistant Canaria Hair Breed (CHB) sheep vaccinated with rIL‐5 in Quil‐A adjuvant, had lower blood eosinophil counts and higher mean worm burdens than control sheep vaccinated with Quil‐A adjuvant alone. In addition, adult worms in IL‐5‐vaccinated sheep were significantly longer with higher eggs in utero in female worms, supporting an active role of eosinophils against adult parasites in CHB sheep. These results confirm that eosinophils can play a direct role in effective control of H contortus infection in sheep and offer a new approach to study immune responses in ruminants.

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Section: Discussionsupporting

confidence: 82%

Section: Several In Vitro Killing or In Vivo Histological And Correlasupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Hernández

Meeusen

Rodrı́guez

et al. 2019

Parasite Immunology

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“…Abomasal tissue samples (approximately 2 cm × 2 cm) were collected at post-mortem from the antropyloric region for histological and immunohistochemistry studies, performed as described previously [ 15 ]. Eosinophil and globule leukocyte (GL) counts were performed in abomasal tissue stained with haematoxylin and eosin (Additional file 2 ), while toluidine blue staining was used to determine mast cell numbers.…”

Section: Methodsmentioning

confidence: 99%

“…The tissue samples taken for immunohistochemistry were used to identify and count the following cell populations: CD4 + , CD8 + , γδTCR + , MHCII + , CD45RA + and Galectin-14 + (Additional file 1 ) [ 15 ]. Cells were counted in 20 fields located in upper and lower third of the mucosa and were expressed in cells/mm 2 , as described above (Additional file 2 ).…”

Section: Methodsmentioning

confidence: 99%

Cellular and humoral immune responses associated with protection in sheep vaccinated against Teladorsagia circumcincta

Machín

Corripio-Miyar

Hernández

et al. 2021

Vet Res

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Due to increased anthelmintic resistance, complementary methods to drugs are necessary to control gastrointestinal nematodes (GIN). Vaccines are an environmentally-friendly and promising option. In a previous study, a Teladorsagia circumcincta recombinant sub-unit vaccine was administered to two sheep breeds with different levels of resistance against GIN. In the susceptible Canaria Sheep (CS) breed, vaccinates harboured smaller worms with fewer eggs in utero than the control group. Here, we extend this work, by investigating the cellular and humoral immune responses of these two sheep breeds following vaccination and experimental infection with T. circumcincta. In the vaccinated CS group, negative associations between antigen-specific IgA, IgG2 and Globule Leukocytes (GLs) with several parasitological parameters were established as well as a higher CD4+/CD8+ ratio than in control CS animals, suggesting a key role in the protection induced by the vaccine. In the more resistant Canaria Hair Breed (CHB) sheep the vaccine did not significantly impact on the parasitological parameters studied and none of these humoral associations were observed in vaccinated CHB lambs, although CHB had higher proportions of CD4+ and CD8+ T cells within the abomasal lymph nodes, suggesting higher mucosal T cell activation. Each of the component proteins in the vaccine induced an increase in immunoglobulin levels in vaccinated groups of each breed. However, levels of immunoglobulins to only three of the antigens (Tci-MEP-1, Tci-SAA-1, Tci-ASP-1) were negatively correlated with parasitological parameters in the CS breed and they may be, at least partially, responsible for the protective effect of the vaccine in this breed. These data could be useful for improving the current vaccine prototype.

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Differences within Churra breed sheep in the early immune response to the infection by Teladorsagia circumcincta

et al. 2020

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Modulation of Haemonchus contortus infection by depletion of γδ+ T cells in parasite resistant Canaria Hair Breed sheep

Cited by 14 publications

References 28 publications

Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Cellular and humoral immune responses associated with protection in sheep vaccinated against Teladorsagia circumcincta

Differences within Churra breed sheep in the early immune response to the infection by Teladorsagia circumcincta

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