Modulation of Guard Cell Turgor and Drought Tolerance by a Peroxisomal Acetate–Malate Shunt

Dong, H.; Bai, Ling; Zhang, Yu; Zhang, Guozeng; Mao, Yanqing; Min, Lulu; Xiang, Fuyou; Qian, Dongdong; Zhu, Xiaohong; Song, Chun‐Peng

doi:10.1016/j.molp.2018.07.008

Cited by 53 publications

(34 citation statements)

References 70 publications

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“…ion (Chen and Liao 2016) and osmotic imbalance as osmoticm (Dong et al 2018). In Arabidopsis, AtMLS (At5g03860) and AtTDT (At5g47560) are induced by NaCl and mannitol, reflecting the roles of the organic acid in abiotic tolerance (Arabidopsis eFP Browser 2.0).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato

Abdelkareem

Thagun

Imanishi

et al. 2019

Plant Biotechnology

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In Solanum lycoperisicum (tomato), a transcription factor of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family, JASMONATE-RESPONSIVE ERF 3 (JRE3), is a closest homolog of JRE4, a master transcriptional regulator of steroidal glycoalkaloid (SGA) biosynthesis. In tomato genome, JRE3 resides in a gene cluster with JRE4 and related JRE1, JRE2, and JRE5, while JRE6 exists as a singleton on a different chromosome. All of the JREs are induced by jasmonates (JAs), whereas sodium chloride (NaCl) treatment drastically increases the expression of the JREs except for JRE4 and JRE6. In this study, to get insights into the regulatory function of the JA-and NaCl-inducible JRE3, a series of genes upregulated by β-estradiol-induced overexpression of JRE3 are identified with microarray analysis in transgenic tomato hairy roots. No gene involved in the SGA pathway has been identified through the screening, confirming the functional distinction between JRE3 and JRE4. Among the JRE3-regulated genes, we characterize the stress-induced expression of genes encoding malate synthase and tonoplast dicarboxylate transporter both involved in malate accumulation. In transient transactivation assay, we reveal that both terminal regions of JRE4, but not a central DNA-binding domain, are indispensable for the induction of a gene involved in the JRE4 regulon. Functional differentiation of the JREs is discussed.

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Section: Discussionmentioning

confidence: 99%

Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato

Abdelkareem

Thagun

Imanishi

et al. 2019

Plant Biotechnology

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“…The coding sequences of target proteins were amplified from cDNAs and inserted into pS1300‐GFP‐nos or pS1300‐Myc‐nos vector to create fusions with GFP or Myc tags on the C terminal, respectively. The target genes are driven by a constitutive promoter (Dong et al , 2018). The point mutation vectors were generated by QuikChange site‐directed mutagenesis kit (Agilent Technologies).…”

Section: Methodsmentioning

confidence: 99%

Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance

Gao

Gong

et al. 2020

Molecular Plant Pathology

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Molecular Plant Pathology. 2020;21:975-984. | 975 wileyonlinelibrary.com/journal/mpp | INTRODUC TI ONPlants prevent pathogen invasion via a two-layered immune system (Dodds and Rathjen, 2010;Dangl et al., 2013). The first layer is pattern-triggered immunity (PTI) governed by membrane-localized pattern recognition receptors (PRRs). PTI is the basal immune response of plants against infections by pathogens (Couto and Zipfel, 2016;Tang et al., 2017). Once pathogens suppress PTI and enter host cells, plants employ the second layer of the immune system to combat pathogens.In this branch of the immune system, intracellular nucleotide-binding Abstract Nucleotide-binding (NB) leucine-rich repeat (LRR) receptors (NLRs) provide resistance against several plant pathogens. We previously cloned the wheat powdery mildew resistance gene Pm21, which encodes a coiled-coil (CC) NLR that confers broad-spectrum resistance against Blumeria graminis f. sp. tritici. Here, we report comprehensive biochemical and functional analyses of Pm21 CC domain in Nicotiana benthamiana. Transient overexpression assay suggested that only the extended CC (eCC, amino acid residues 1-159) domain has cell-death-inducing activity, whereas the CC-containing truncations, including CC-NB and CC-NB-LRR, do not induce celldeath responses. Coimmunoprecipitation (Co-IP) assay showed that the eCC domain self-associates and interacts with the NB and LRR domains in planta. These results imply that the activity of the eCC domain is inhibited by the intramolecular interactions of different domains in the absence of pathogens. We found that the LRR domain plays a crucial role in D491V-mediated full-length (FL) Pm21 autoactivation. Some mutations in the CC domain leading to the loss of Pm21 resistance to powdery mildew impaired the CC activity of cell-death induction. Two mutations (R73Q and E80K) interfered with D491V-mediated Pm21 autoactivation without affecting the cell-death-inducing activity of the eCC domain. Notably, some susceptible mutants harbouring mutations in the CC domain still exhibited cell-death-inducing activity. Taken together, these results implicate the CC domain of Pm21 in cell-death signalling and disease-resistance signalling, which are potentially independent of each other. K E Y W O R D S CC domain, cell death, disease resistance, intramolecular interaction, Pm21 S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Gao A, Hu M, Gong Y, et al. Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance. Molecular Plant Pathology. 2020;21:975-984.

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“…4). ACN1 was recently shown to be the starting point of the acetate-malate shunt, a process that converts acetate to malate in peroxisomes and, very interestingly, modulates guard cell turgor and drought tolerance (Dong et al, 2018). ACN1 was recently shown to be the starting point of the acetate-malate shunt, a process that converts acetate to malate in peroxisomes and, very interestingly, modulates guard cell turgor and drought tolerance (Dong et al, 2018).…”

Section: The Glyoxylate Cycle and Acetate-malate Shuntmentioning

confidence: 99%

“…ACN1 only consumes a small fraction of the total cellular acetate, yet it affects primary metabolism and prevents carbon leakage from peroxisomes during lipid mobilization in seedlings (Allen et al, 2011). ACN1 was recently shown to be the starting point of the acetate-malate shunt, a process that converts acetate to malate in peroxisomes and, very interestingly, modulates guard cell turgor and drought tolerance (Dong et al, 2018).…”

Section: The Glyoxylate Cycle and Acetate-malate Shuntmentioning

confidence: 99%

Peroxisomes: versatile organelles with diverse roles in plants

Pan

Wang

et al. 2019

New Phytologist

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Summary Peroxisomes are small, ubiquitous organelles that are delimited by a single membrane and lack genetic material. However, these simple‐structured organelles are highly versatile in morphology, abundance and protein content in response to various developmental and environmental cues. In plants, peroxisomes are essential for growth and development and perform diverse metabolic functions, many of which are carried out coordinately by peroxisomes and other organelles physically interacting with peroxisomes. Recent studies have added greatly to our knowledge of peroxisomes, addressing areas such as the diverse proteome, regulation of division and protein import, pexophagy, matrix protein degradation, solute transport, signaling, redox homeostasis and various metabolic and physiological functions. This review summarizes our current understanding of plant peroxisomes, focusing on recent discoveries. Current problems and future efforts required to better understand these organelles are also discussed. An improved understanding of peroxisomes will be important not only to the understanding of eukaryotic cell biology and metabolism, but also to agricultural efforts aimed at improving crop performance and defense.

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Modulation of Guard Cell Turgor and Drought Tolerance by a Peroxisomal Acetate–Malate Shunt

Cited by 53 publications

References 70 publications

Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato

Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato

Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance

Peroxisomes: versatile organelles with diverse roles in plants

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