Modulation of growth, immune response, and immune-antioxidant related gene expression of Nile tilapia (Oreochromis niloticus) reared under biofloc system using mango peel powder

Outama, Piyatida; Xuan, Chinh Le; Wannavijit, Supreya; Lumsangkul, Chompunut; Linh, Nguyen Vu; Montha, Napatsorn; Tongsiri, Sudaporn; Chitmanat, Chanagun; Doan, Hien Van

doi:10.1016/j.fsi.2022.09.031

Cited by 5 publications

(6 citation statements)

References 102 publications

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“…Glutathione S-transferase (GST) detoxifies electrophiles, enhancing their elimination [94]. In this study, we found dietary CH-supplemented significantly increased antioxidant gene (GSR, GPX, GST-α) expression in tilapia liver and intestine, similar to previous tilapia studies [13] and golden pompano [57]. This suggests that CH may mitigate oxidative damage.…”

Section: Discussionsupporting

confidence: 84%

“…Antioxidant supplements can improve fish health by reducing oxidative stress. Glutathione peroxidase (GPX) and glutathione reductase (GSR) remove hydrogen peroxide using glutathione [13,93]. Glutathione S-transferase (GST) detoxifies electrophiles, enhancing their elimination [94].…”

Section: Discussionmentioning

confidence: 99%

“…Reactions contained 1 µL cDNA (100 ng), 0.4 µL each primer (10 µM), 10 µL 2× SYBR Green Mastermix (BIO-RAD, USA) and nuclease-free water to 20 µL total volume. qPCR was performed on a CFX ConnectTM system (BIO-RAD, USA) as described previously [13]. Relative mRNA levels were calculated using the 2 −∆∆Ct method [102] with 18S rRNA as the internal reference gene.…”

Section: Histopathology Analysismentioning

confidence: 99%

“…However, concerns over antibiotic usage in aquaculture have led to investigating replacements to reduce reliance on these treatments [7,8]. This has sparked great interest in finding new, innovative feed additives for tilapia, such as probiotics [9,10], prebiotics [11][12][13], and synbiotics [14,15]. These additives have been reported to enhance growth performance, health status, immune function, antioxidant defenses, and immune-related gene expression in tilapia.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Shrimp Shell-Derived Chitosan on Growth, Immunity, Intestinal Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System

Linh,

Lubis,

Dinh-Hung

et al. 2024

Marine Drugs

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Chitosan (CH) shows great potential as an immunostimulatory feed additive in aquaculture. This study evaluates the effects of varying dietary CH levels on the growth, immunity, intestinal morphology, and antioxidant status of Nile tilapia (Oreochromis niloticus) reared in a biofloc system. Tilapia fingerlings (mean weight 13.54 ± 0.05 g) were fed diets supplemented with 0 (CH0), 5 (CH5), 10 (CH10), 20 (CH20), and 40 (CH40) mL·kg−1 of CH for 8 weeks. Parameters were assessed after 4 and 8 weeks. Their final weight was not affected by CH supplementation, but CH at 10 mL·kg−1 significantly improved weight gain (WG) and specific growth rate (SGR) compared to the control (p < 0.05) at 8 weeks. Skin mucus lysozyme and peroxidase activities were lower in the chitosan-treated groups at weeks 4 and 8. Intestinal villi length and width were enhanced by 10 and 20 mL·kg−1 CH compared to the control. However, 40 mL·kg−1 CH caused detrimental impacts on the villi and muscular layer. CH supplementation, especially 5–10 mL·kg−1, increased liver and intestinal expressions of interleukin 1 (IL-1), interleukin 8 (IL-8), LPS-binding protein (LBP), glutathione reductase (GSR), glutathione peroxidase (GPX), and glutathione S-transferase (GST-α) compared to the control group. Overall, dietary CH at 10 mL·kg−1 can effectively promote growth, intestinal morphology, innate immunity, and antioxidant capacity in Nile tilapia fingerlings reared in biofloc systems.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Histopathology Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Shrimp Shell-Derived Chitosan on Growth, Immunity, Intestinal Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System

Linh,

Lubis,

Dinh-Hung

et al. 2024

Marine Drugs

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“…Lysozyme activities in skin mucus and serum were evaluated according to procedures described previously [39]. The lysozyme activity was measured by comparison with a standard and expressed in units of µg mL −1 of serum.…”

Section: Lysozyme and Peroxidase Activity Assaymentioning

confidence: 99%

Black Soldier Fly (Hermetia illucens) Larvae Meal: A Sustainable Alternative to Fish Meal Proven to Promote Growth and Immunity in Koi Carp (Cyprinus carpio var. koi)

Linh,

Wannavijit,

Tayyamath

et al. 2024

Fishes

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Insect meal has shown promise as a potentially sustainable source of nutrients for aquafeeds, offering an alternative to expensive and ecologically undesirable ingredients, in the context of population explosion and climate change. Despite this promising outlook, its effects on fish growth and immune responses remain to be thoroughly investigated. Our scientific goal was to experimentally test responses to replacements of the fish meal with a protein source derived from black soldier fly larvae meal (BSFLM). Possible impacts on growth, immunological response, and the expression of selected immune-system related genes were evaluated in Koi carp (Cyprinus carpio var. koi) using a biofloc culture system. Three hundred fish (20.0 ± 0.2 g) were allocated into five groups: a control group receiving a basal diet containing 0 g kg−1 BSFLM and four experimental groups in which fish meal was replaced with 50, 100, 150, and 200 g kg−1 BSFLM for eight weeks. After 4 weeks of feeding, there were no statistically significant differences in specific growth rate (SGR), feed conversion ratio (FCR), and survival rate between fish fed BSFLM-enriched diets at 50, 100, 150 g kg−1 and a control (0 g kg−1 BSFLM) diet. However, fish fed 200 g kg−1 BSFLM showed significantly improved weight gain (WG) and SGR compared to the control after 4 weeks; this difference persisted through 8 weeks (p < 0.05). After eight weeks, there was a moderate to weak negative linear regression shown in FCR (r = 0.470) and SR (r = 0.384), respectively, with the BSFLM levels, but significant and highly correlated linear relationships were observed in WG (r = 0.917) and SGR (r = 0.912). Immunological response analysis showed slight changes in lysozyme and peroxidase levels by replacing fish meal with BSFLM, but these apparent differences were not significantly related to experimental diets. Interestingly, mRNA transcripts of immune-related genes (TNF-α, TGF-β, IL1, IL10, and hsp70) were upregulated in the groups receiving higher amounts of BSFLM, with statistically significant differences observed in certain comparisons. Our findings reveal that fish meal can be effectively replaced by BSFLM, and that this not only has a positive effect on immune-related gene expression in Koi carp, but also on growth rate, pointing to the future potential role of BSFLM as an alternative fish meal protein in aquafeed formulation.

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Triphala involved in reducing the susceptibility of Nile tilapia (Oreochromis niloticus) fingerlings to Saprolegnia ferax infection by boosting immune and antioxidant responses, improving growth performance, histological improvement, and gene expression indicators

Jastaniah,

Hassoubah,

Aljahdali

et al. 2024

Aquacult Int

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Modulation of growth, immune response, and immune-antioxidant related gene expression of Nile tilapia (Oreochromis niloticus) reared under biofloc system using mango peel powder

Cited by 5 publications

References 102 publications

Effects of Shrimp Shell-Derived Chitosan on Growth, Immunity, Intestinal Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System

Effects of Shrimp Shell-Derived Chitosan on Growth, Immunity, Intestinal Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System

Black Soldier Fly (Hermetia illucens) Larvae Meal: A Sustainable Alternative to Fish Meal Proven to Promote Growth and Immunity in Koi Carp (Cyprinus carpio var. koi)

Triphala involved in reducing the susceptibility of Nile tilapia (Oreochromis niloticus) fingerlings to Saprolegnia ferax infection by boosting immune and antioxidant responses, improving growth performance, histological improvement, and gene expression indicators

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