Modulation of GmFAD3 expression alters responses to abiotic stress in soybean

Abstract

FAD3 play important roles in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of FAD3 in drought, salinity and heat stress tolerance is lacking in soybean. The present study assessed the functional role of fatty acid desaturase 3 to abiotic stress responses in soybean. We used Bean Pod Mottle Virus -based vector to alter expression of Glycine max omega-3 fatty acid desaturase . Higher levels of recombinant BPMV-GmFAD3 transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3 in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. FAD3 overexpressing plants showed higher levels of chlorophyll content, leaf SPAD value, relative water content, chlorophyll fluorescence, transpiration rate, carbon assimilation rate, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from current study revealed that GmFAD3 overexpressing soybean plants exhibited drought and salinity stress tolerance although tolerance to heat stress was reduced. On the other hand, soybean plants silenced for GmFAD3 exhibited tolerance to heat stress, but were vulnerable to drought and salinity stress

Ampelopsin Confers Endurance and Rehabilitation Mechanisms in Glycine max cv. Sowonkong under Multiple Abiotic Stresses

“…Moreover, we observed the expression of fatty acid desaturase 3 (FAD3) enzymes, which catalyze the conversion of linoleic acid to α-linolenic acid. Recent studies showed that fatty acid overexpression improved cold, drought and osmotic stress endurance in tobacco, soybean and tomato plants [94][95][96]. Subsequently, from these findings, we can come to the conclusion that AMP application may cause the activation of phospholipases, as well as phospholipid derived molecules, which are involved in plant defense systems [97].…”

Salvianolic Acid Modulates Physiological Responses and Stress-Related Genes That Affect Osmotic Stress Tolerance in Glycine max and Zea mays

“…Conversely, SAL-treated seedlings were less affected by this stress and exhibited a remarkably higher level of fatty acids. Singh et al ( 2020 ) indicated that fatty acids boosted drought and salinity stress tolerance in soybean plants. Therefore, these data suggest that SAL utilization may induce the activation of phospholipases and phospholipid-derived molecules, which are engaged in plant protection mechanisms (Hou et al, 2016 ).…”